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中国精品科技期刊2020
陈强,王璐,徐峥嵘,等. 玉米植物糖原超声辅助提取工艺优化及其生物活性评价[J]. 食品工业科技,2024,45(19):177−186. doi: 10.13386/j.issn1002-0306.2023070133.
引用本文: 陈强,王璐,徐峥嵘,等. 玉米植物糖原超声辅助提取工艺优化及其生物活性评价[J]. 食品工业科技,2024,45(19):177−186. doi: 10.13386/j.issn1002-0306.2023070133.
CHEN Qiang, WANG Lu, XU Zhengrong, et al. Optimization of Ultrasound-assisted Extraction of Phytoglycogen from Sweet Corn and Its Bioactivity Evaluation[J]. Science and Technology of Food Industry, 2024, 45(19): 177−186. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070133.
Citation: CHEN Qiang, WANG Lu, XU Zhengrong, et al. Optimization of Ultrasound-assisted Extraction of Phytoglycogen from Sweet Corn and Its Bioactivity Evaluation[J]. Science and Technology of Food Industry, 2024, 45(19): 177−186. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023070133.

玉米植物糖原超声辅助提取工艺优化及其生物活性评价

Optimization of Ultrasound-assisted Extraction of Phytoglycogen from Sweet Corn and Its Bioactivity Evaluation

  • 摘要: 本文主要探究超声辅助法从甜玉米中提取植物糖原(phytoglycogen,PG)的最佳工艺并对其理化性质和生物活性进行初步评价。该实验以PG的得率为指标,考查固液比、超声功率和超声时间对PG得率的影响,并利用正交试验优化工艺参数。在此基础上,研究了超声辅助法制备得到的PG的理化性能,并开展PG的体外抗氧化、细胞毒性、凋亡以及溶血评价。结果表明:正交试验优化后得到最佳工艺为固液比1:9(g/mL)、超声功率160 W、超声时间80 min,在此条件下实际得率为14.31%±0.38%;表征结果显示超声辅助法获取的PG颗粒尺寸低于传统水提醇沉法;体外抗氧化实验表明PG具有一定的抗氧化能力,尤其是对DPPH自由基更为敏感,在20 mg/mL时清除率达到54.58%±1.39%;生物安全性评价结果显示不同浓度的PG在与小鼠成纤维细胞(3T3)孵育24或48 h后,细胞存活率均高于95%;同时,PG也没有对细胞产生明显的凋亡效应,在最大浓度时,凋亡率低于7%;此外,在共孵育2 h后,PG对红细胞造成的溶血率也远低于国家标准(5%),这些结果证实了PG对正常细胞和红细胞均有良好的生物相容性。总体上,本研究表明超声辅助法有助于提高PG的得率和理化性能,同时,也证实了PG是一种无毒的天然纳米颗粒,在药物或食品因子递送方面具有较大的应用潜力。

     

    Abstract: The optimal extraction process of phytoglycogen (PG) from sweet corn by ultrasound-assisted method was studied, and the physicochemical properties and biological activities of PG were evaluated in this paper. The effects of solid-liquid ratio, ultrasonic power and ultrasonic time on the yield of PG were investigated and the process parameters were further optimized by orthogonal experiment. Furthermore, the physicochemical properties of PG obtained by ultrasound-assisted method were investigated. Meanwhile, the antioxidation, cytotoxicity, apoptosis and hemolysis of PG were evaluated in vitro. The results showed that the optimal extraction conditions were as follows: Solid-liquid ratio of 1:9 (g/mL), ultrasonic power of 160 W and ultrasonic time of 80 min, the actual yield was 14.31%±0.38%. The PG particle size obtained by ultrasound-assisted method was lower than that obtained by traditional water extraction and alcohol precipitation method. In vitro antioxidant experiment showed that PG had a certain antioxidant capacity, especially it was more sensitive to DPPH free radicals, and the clearance rate reached 54.58%±1.39% at 20 mg/mL. The results of biosafety evaluation showed that the survival rate of mouse fibroblasts (3T3) was higher than 95% after incubation with different concentrations of PG for 24 or 48 h. Meanwhile, PG had no obvious apoptotic effect on cells, and the apoptotic rate was lower than 7% at the maximum concentration. After 2 h of co-incubation, the hemolysis rate caused by PG on red blood cells was also much lower than the national standard (5%), which confirmed that PG had good biocompatibility with normal cells and red blood cells. Overall, this study showed that ultrasound-assisted method could improve the yield and physicochemical properties of PG, and also confirmed that PG was a non-toxic natural nanoparticle, which had great application potential in drug or food factor delivery.

     

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