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中国精品科技期刊2020
周元龙,宋年年,易宇欣,等. 不同提取方法对罗布麻多糖理化性质及抗氧化活性的影响[J]. 食品工业科技,2024,45(19):40−48. doi: 10.13386/j.issn1002-0306.2023090310.
引用本文: 周元龙,宋年年,易宇欣,等. 不同提取方法对罗布麻多糖理化性质及抗氧化活性的影响[J]. 食品工业科技,2024,45(19):40−48. doi: 10.13386/j.issn1002-0306.2023090310.
ZHOU Yuanlong, SONG Niannian, YI Yuxin, et al. Effects of Different Extraction Methods on Physicochemical Properties and Antioxidant Activity of Apocynum venetum Polysaccharides[J]. Science and Technology of Food Industry, 2024, 45(19): 40−48. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090310.
Citation: ZHOU Yuanlong, SONG Niannian, YI Yuxin, et al. Effects of Different Extraction Methods on Physicochemical Properties and Antioxidant Activity of Apocynum venetum Polysaccharides[J]. Science and Technology of Food Industry, 2024, 45(19): 40−48. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090310.

不同提取方法对罗布麻多糖理化性质及抗氧化活性的影响

Effects of Different Extraction Methods on Physicochemical Properties and Antioxidant Activity of Apocynum venetum Polysaccharides

  • 摘要: 本文以罗布麻叶为研究对象,探讨热水提取(HWE)、超声辅助提取(UE)、纤维素酶提取(CE)、果胶酶提取(PE)和超声微波协同提取(UME)对罗布麻多糖(Apocynum venetum Polysaccharides,AVP)理化性质及活性的影响。结果表明,多糖得率为:AVP-PE(13.6%)>AVP-CE(13.4%)>AVP-UME(12.4%)>AVP-UE(12.1%)>AVP-HWE(9.2%)。不同提取法所得多糖均主要由不同摩尔比的阿拉伯糖、葡萄糖及半乳糖组成,然而各单糖占比有所不同,其中AVP-HWE及AVP-CE中阿拉伯糖占比最高,另三种多糖中葡萄糖占比最高。不同多糖均显出了典型的多糖类化合物红外吸收峰,均不具有三螺旋结构,且表现出不同的表面结构。各多糖均具有较强的抗氧化活性,其中,AVP-HWE具有最强的DPPH及ABTS+自由基清除能力,半抑制浓度分别为0.219及0.072 mg/mL;AVP-UE具有最强的羟自由基清除能力,半抑制浓度为0.298 mg/mL;AVP-CE具有最强的亚铁离子螯合能力,半抑制浓度为0.107 mg/mL;不同多糖的总还原能力差距较小。综上,不同提取方法对AVP的结构及活性有显著的影响,UE和CE更适合罗布麻多糖的提取。本文将为罗布麻叶多糖资源的开发利用提供参考。

     

    Abstract: In this paper, the effects of hot water extraction (HWE), ultrasonic-assisted extraction (UE), cellulase assisted extraction (CE), pectinase assisted extraction (PE), and ultrasonic microwave assisted extraction (UME) on the physicochemical properties and activities of polysaccharide from Apocinae venetiana (AVP) were studied. The results showed that the extraction rate in the order of AVP-PE (13.6%)>AVP-CE (13.4%)>AVP-UME (12.4%)>AVP-UE (12.1%)>AVP-HWE (9.2%). The polysaccharides obtained by different extraction methods were mainly composed of arabinose, glucose and galactose in a different molar ratio, however, the proportion of each monosaccharide was different. The arabinose was the predominant monosaccharide for AVP-HWE and AVP-CE, and glucose for the others. Different polysaccharides exhibited typical infrared absorption peaks of polysaccharide compounds without a triple helix structure, and showed different surface structures. The polysaccharides demonstrated strong antioxidant activity, among which AVP-HWE had the strongest DPPH and ABTS+ free radicals scavenging ability, with half inhibition concentrations (IC50) of 0.219 and 0.072 mg/mL, respectively. AVP-UE had the strongest hydroxyl radical scavenging ability, with IC50 of 0.298 mg/mL. AVP-CE had the strongest ferrous ion chelating ability, with IC50 of 0.107 mg/mL, and there were few differences for the total reducing power between different polysaccharides. In summary, different extraction methods have a significant impact on the structure and activity of AVP, and UE and CE are more suitable for the extraction of AVP. This paper may be providing reference for the development and utilization of polysaccharides resources from Apocynum venetum leaves.

     

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