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中国精品科技期刊2020
晋欣,张楠,梅晓亮,等. 广藿香水提物对溃疡性结肠炎小鼠肠屏障的影响[J]. 食品工业科技,2024,45(24):332−341. doi: 10.13386/j.issn1002-0306.2023100226.
引用本文: 晋欣,张楠,梅晓亮,等. 广藿香水提物对溃疡性结肠炎小鼠肠屏障的影响[J]. 食品工业科技,2024,45(24):332−341. doi: 10.13386/j.issn1002-0306.2023100226.
JIN Xin, ZHANG Nan, MEI Xiaoliang, et al. Effect of Pogostemon cablin Aqueous Extract on Gut Barrier in Mouse Model of Ulcerative Colitis[J]. Science and Technology of Food Industry, 2024, 45(24): 332−341. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100226.
Citation: JIN Xin, ZHANG Nan, MEI Xiaoliang, et al. Effect of Pogostemon cablin Aqueous Extract on Gut Barrier in Mouse Model of Ulcerative Colitis[J]. Science and Technology of Food Industry, 2024, 45(24): 332−341. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100226.

广藿香水提物对溃疡性结肠炎小鼠肠屏障的影响

Effect of Pogostemon cablin Aqueous Extract on Gut Barrier in Mouse Model of Ulcerative Colitis

  • 摘要: 目的:基于肠屏障研究广藿香水提物对溃疡性结肠炎(ulcerative colitis,UC)小鼠改善的作用及机制。方法:将小鼠随机分为空白组、模型组、美沙拉嗪组、广藿香水提物低、高剂量组;自由饮用2.5%葡聚糖硫酸钠(dextran sodium sulfate,DSS)10 d诱导小鼠UC模型,造模同时灌胃干预;检测血清白介素6(interleukin-6,IL-6)、白介素1β(IL-1β)和肿瘤坏死因子α(tumor necrosis factor α,TNF-α)水平;苏木素-伊红和高碘酸-席夫染色观察结肠病理变化;免疫组化法检测结肠紧密连接蛋白1(znula ocludens-1,ZO-1)、闭合连接蛋白1(occludin-1)和粘蛋白2(mucin 2,MUC2)蛋白表达;高通量测序技术分析结肠内容物肠道菌群变化。结果:DSS诱导的小鼠经广藿香水提物干预后,血清IL-6、IL-1β和TNF-α水平明显降低(P<0.05,P<0.01);结肠炎性浸润、上皮细胞结构破坏和杯状细胞数量减少等病理得到改善;结肠ZO-1、occludin-1和MUC2蛋白表达明显升高(P<0.05,P<0.01);肠道菌群α多样性sobs、Shannon和heip指数明显升高(P<0.05,P<0.01);肠道菌群β多样性主成分分析、主坐标分析和非度量多维尺度分析趋于健康小鼠;肠道菌群属水平优势物种隆布茨菌属(Romboutsia)、双歧杆菌属(Bifidobacterium)、布劳提菌属(Blautia)和unclassified_f__Lachnospiraceae丰度明显纠正(P<0.05,P<0.01)。结论:广藿香水提物对于DSS诱导的UC小鼠具有明确的改善作用,可能是其通过改善肠屏障而实现。

     

    Abstract: Objective: To explore the ameliorative effects and mechanisms of Pogostemon cablin aqueous extract (PCAE) on mice with ulcerative colitis (UC) based on gut barrier research. Methods: Mice were randomly divided into five groups: Control, model, mesalazine, low-dose of PCAE and high-dose of PCAE groups. A UC model was induced in mice by freely drinking 2.5% dextran sodium sulfate (DSS) for 10 d, with simultaneous oral administration of interventions. The study measured levels of serum interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor α (TNF-α). Pathological alterations in the colon were observed using hematoxylin-eosin and periodic acid-schiff staining. The expression of the colonic tight junction proteins zonula occludens-1 (ZO-1), occludin-1, and mucin 2 (MUC2) were assessed via immunohistochemistry. High-throughput sequencing technology was utilized to analyze changes in the gut microbiota of colonic contents. Results: Following intervention with PCAE, DSS-induced mice exhibited a significant reduction in serum levels of IL-6, IL-1β, and TNF-α (P<0.05, P<0.01). Pathological improvements in colon inflammation, epithelial cell structural damage, and reduced goblet cell numbers were observed. Additionally, the expression of colonic ZO-1, occludin-1, and MUC2 proteins significantly increased (P<0.05, P<0.01). Furthermore, the α-diversity indices of the gut microbiota including sobs, Shannon, and heip exhibited a marked increase (P<0.05, P<0.01), while the β-diversity analysis through principal component analysis, principal co-ordinates analysis, and non-metric multidimensional scaling analysis tended towards that of healthy mice. Notably, there was a significant correction in the abundance of dominant species at the genus level, including Romboutsia, Bifidobacterium, Blautia, and unclassified_f__Lachnospiraceae (P<0.05, P<0.01). Conclusion: PCAE exhibits a clear ameliorative effect on DSS-induced UC in mice, potentially achieved through the improvement of the gut barrier.

     

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