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中国精品科技期刊2020
井勇慧,李桥,康丽,等. 响应面法优化山楂多酚微粒制备工艺[J]. 食品工业科技,2024,45(20):187−195. doi: 10.13386/j.issn1002-0306.2023100230.
引用本文: 井勇慧,李桥,康丽,等. 响应面法优化山楂多酚微粒制备工艺[J]. 食品工业科技,2024,45(20):187−195. doi: 10.13386/j.issn1002-0306.2023100230.
JING Yonghui, LI Qiao, KANG Li, et al. Optimization of Preparation Process for Hawthorn Polyphenol Microparticles Based on Response Surface Methodology[J]. Science and Technology of Food Industry, 2024, 45(20): 187−195. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100230.
Citation: JING Yonghui, LI Qiao, KANG Li, et al. Optimization of Preparation Process for Hawthorn Polyphenol Microparticles Based on Response Surface Methodology[J]. Science and Technology of Food Industry, 2024, 45(20): 187−195. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023100230.

响应面法优化山楂多酚微粒制备工艺

Optimization of Preparation Process for Hawthorn Polyphenol Microparticles Based on Response Surface Methodology

  • 摘要: 本研究依据聚电解质自组装原理将山楂多酚制备为山楂多酚微粒(Hawthorn polyphenol microparticles,HPM)。通过Plackett-Burman联用Box-Behnken设计,以山楂多酚的包封率和载药量为响应值对HPM的制备工艺因素进行研究。采用Plackett-Burman设计筛选对实验结果影响较为显著的因素(制备时间、硫酸浓度和包封材料比例)进行Box-Behnken响应曲面优化实验。利用SEM和TEM观测HPM的形貌,并利用模拟胃肠液研究HPM的胃肠道释放动力学。结果表明:制备时间、硫酸浓度和包封材料比例对HPM的制备具有显著影响,制备时间为3 h,硫酸浓度为0.0254%,壳聚糖与海藻酸钠比例为4:1时HPM的包封率为96.27%±1.37%。对HPM的形貌研究显示,其为不规则的圆形颗粒且分散性良好。HPM在模拟肠环境中的释放速度大于模拟胃环境。其在胃液和肠液中的释放分别属于零级和一级动力学。综上所述,本研究制备出一种包封率、载药量较高且分散性良好的HPM。并且制备的HPM可以降低胃肠道对山楂多酚的降解,为山楂多酚的食品添加提供了新的思路。

     

    Abstract: This study synthesized hawthorn polyphenol microparticles (HPM) based on the polyelectrolyte self-assembly technology. Plackett-burman combined with Box-Behnken design was used to study the factors affecting the preparation of HPM in response to the encapsulation efficiency and drug loading of hawthorn polyphenols. The Plackett-Burman design was used to screen for factors that significantly affect the outcomes, such as preparation time, sulfuric acid concentration, and the ratio of encapsulating materials, which were optimized through Box-Behnken response surface methodology. The morphology of HPM was examined using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and their gastrointestinal release kinetics were assessed using simulated gastric and intestinal fluids. The results indicated that the preparation time, sulfuric acid concentration, and the ratio of encapsulating materials significantly effect the fabrication of HPM. An encapsulation efficiency of 96.27%±1.37% was achieved with a preparation time of 3 h, a sulfuric acid concentration of 0.0254%, and a 4:1 ratio of chitosan to sodium alginate. Morphological analysis of HPM revealed irregularly shaped, well-dispersed spherical particles. The release rate of HPM in simulated intestinal fluid was higher than in gastric fluid, with the release kinetics corresponding to zero-order in gastric fluid and first-order in intestinal fluid. In summary, this study successfully produced HPM with a high encapsulation efficiency, substantial drug loading, and good dispersion. The HPM formulated could reduce the degradation of hawthorn polyphenols in the gastrointestinal tract, offering a novel approach for incorporating hawthorn polyphenols into food products.

     

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