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中国精品科技期刊2020
王华,孟晶晶,马云啸,等. 荞麦蜂花粉多糖的纯化、表征及其在体外消化过程中抗氧化活性的变化[J]. 食品工业科技,2024,45(19):58−65. doi: 10.13386/j.issn1002-0306.2023110040.
引用本文: 王华,孟晶晶,马云啸,等. 荞麦蜂花粉多糖的纯化、表征及其在体外消化过程中抗氧化活性的变化[J]. 食品工业科技,2024,45(19):58−65. doi: 10.13386/j.issn1002-0306.2023110040.
WANG Hua, MENG Jingjing, MA Yunxiao, et al. Purification and Characterization of Buckwheat Bee Pollen Polysaccharide and Changes of Its Antioxidant Activity during in Vitro Digestion[J]. Science and Technology of Food Industry, 2024, 45(19): 58−65. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110040.
Citation: WANG Hua, MENG Jingjing, MA Yunxiao, et al. Purification and Characterization of Buckwheat Bee Pollen Polysaccharide and Changes of Its Antioxidant Activity during in Vitro Digestion[J]. Science and Technology of Food Industry, 2024, 45(19): 58−65. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110040.

荞麦蜂花粉多糖的纯化、表征及其在体外消化过程中抗氧化活性的变化

Purification and Characterization of Buckwheat Bee Pollen Polysaccharide and Changes of Its Antioxidant Activity during in Vitro Digestion

  • 摘要: 本论文旨在研究荞麦蜂花粉多糖(BWPP)在模拟体外胃肠消化过程中的抗氧化活性。采用DEAE-52纤维素柱对粗多糖进行分级纯化,经体外模拟胃、肠消化研究其在体外消化过程中的抗氧化活性的变化规律。结果表明经DEAE-52纤维素柱层析洗脱纯化后得到总糖含量最高组分BWPP-1,其主要由Glc、Xyl、Ara等组成,且红外光谱显示BWPP-1具有明显的多糖特征吸收峰。BWPP和BWPP-1在进行体外模拟胃肠消化时,模拟胃液消化60 min时,BWPP和BWPP-1的ABTS+·清除率均达到最大值,分别为86.39%±1.28%、90.38%±2.78%,与其他消化时间的存在显著差异(P<0.05);60 min时对DPPH·清除率达到最大值,分别为86.86%±0.11%、89.58%±1.67%,且二者差异不显著(P>0.05);模拟肠液消化时,BWPP和BWPP-1对ABTS+·清除率在60 min时达到最大值为88.67%±0.40%和91.82%±2.77%,且二者差异不显著(P>0.05);对DPPH·清除率在240 min时达到最大值57.11%±0.06%和65.67%±3.67%,二者差异显著(P<0.05);而在消化过程中,对铁离子还原能力均较弱。由此可得,BWPP和BWPP-1具有较好的ABTS+·和DPPH·清除能力,且胃液消化时DPPH·清除能力略高于肠液消化,而肠液消化时的ABTS+·清除能力略高于胃液消化。本研究为以荞麦蜂花粉为基料的功能性产品的开发提供理论依据。

     

    Abstract: This paper aimed to investigate the antioxidant activity of buckwheat bee pollen polysaccharides (BWPP) during the in vitro gastrointestinal digestion. The crude polysaccharides were purified using DEAE-52 cellulose column and their antioxidant activity during in vitro simulated gastric and intestinal digestion was studied. The results showed that after purification by DEAE-52 cellulose column chromatography, the highest total sugar content component BWPP-1 was obtained, which was mainly composed of Glc, Xyl, Ara, etc. The infrared spectrum showed that BWPP-1 had obvious polysaccharide characteristic absorption peaks. During the simulated in vitro gastrointestinal digestion, the ABTS+· scavenging rates of BWPP and BWPP-1 reached their maximum values at 60 minutes of simulated gastric juice digestion, which were 86.39%±1.28% and 90.38%±2.78%, respectively. There was a significant difference compared to other digestion times (P<0.05). The DPPH· clearance rates reached their maximum values at 60 minutes, which were 86.86%±0.11% and 89.58%±1.67%, respectively, and the difference between the two was not significant (P>0.05). When simulating intestinal digestion, the maximum clearance rates of ABTS+· by BWPP and BWPP-1 reached 88.67%±0.40% and 91.82%±2.77% at 60 minutes, and the difference between the two was not significant (P>0.05). The DPPH· scavenging rates reached their peak values at 240 minutes of 57.11%±0.06% and 65.67%±3.67%, with significant difference (P<0.05). During the digestion process, the ability to reduce iron ions was relatively weak. Based on these findings, it can be concluded that BWPP and BWPP-1 have good ABTS+· and DPPH· scavenging abilities, and the DPPH· scavenging ability during gastric digestion is slightly higher than that during intestinal digestion, while the ABTS+· scavenging ability during intestinal digestion is slightly higher than that during gastric digestion. This study provides a theoretical basis for the development of functional products based on buckwheat bee pollen.

     

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