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中国精品科技期刊2020
孙嘉莉,江峻峰,牟晓娟,等. 沙棘果皮渣总黄酮的纯化、成分分析及对胰脂肪酶的抑制作用[J]. 食品工业科技,2024,45(19):206−215. doi: 10.13386/j.issn1002-0306.2023110077.
引用本文: 孙嘉莉,江峻峰,牟晓娟,等. 沙棘果皮渣总黄酮的纯化、成分分析及对胰脂肪酶的抑制作用[J]. 食品工业科技,2024,45(19):206−215. doi: 10.13386/j.issn1002-0306.2023110077.
SUN Jiali, JIANG Junfeng, MOU Xiaojuan, et al. Purification and Component Analysis of Total Flavonoids from Sea Buckthorn Peel Residue and Its Anti-Pancreatic Lipase[J]. Science and Technology of Food Industry, 2024, 45(19): 206−215. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110077.
Citation: SUN Jiali, JIANG Junfeng, MOU Xiaojuan, et al. Purification and Component Analysis of Total Flavonoids from Sea Buckthorn Peel Residue and Its Anti-Pancreatic Lipase[J]. Science and Technology of Food Industry, 2024, 45(19): 206−215. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110077.

沙棘果皮渣总黄酮的纯化、成分分析及对胰脂肪酶的抑制作用

Purification and Component Analysis of Total Flavonoids from Sea Buckthorn Peel Residue and Its Anti-Pancreatic Lipase

  • 摘要: 为了明确沙棘果皮渣中总黄酮纯化物(TFSE)对胰脂肪酶的抑制作用,本文通过静态及动态吸附解析实验,优化了AB-8大孔树脂纯化沙棘果皮渣总黄酮粗提物(TFCE)的条件;采用超高效液相色谱-高分辨质谱联用技术(UPLC-Q-MS)分析TFSE组成成分;对硝基苯基丁酸酯法结合酶反应动力学探究TFSE对胰脂肪酶抑制效果及抑制类型。结果表明,AB-8大孔树脂最佳纯化条件为上样液浓度2.2 mg/mL、上样液pH 5.0、上样体积140 mL,上样流速1.0 mL/min;洗脱液乙醇体积分数75%、洗脱剂用量120 mL、洗脱流速1.5 mL/min,在此条件下分离纯化,TFSE纯度由9.786%提高到50.204%;从TFSE中鉴定出39种酚类化合物,以芦丁、儿茶素、槲皮素-3-O-葡萄糖苷等黄酮类化合物为主;TFSE对胰脂肪酶有较好的抑制作用,其IC50值为12.46 mg/mL,并通过Lineweaver-Burk双倒数作图法测出其抑制类型为非竞争性抑制,抑制常数Ki=10.21 mg/mL。TFSE具有良好的胰脂肪酶抑制活性,对于提高沙棘果皮渣产品的附加值具有重要意义。

     

    Abstract: In order to clarify the inhibition of total flavonoids purified extract (TFSE) of sea buckthorn peel residue on pancreatic lipase, this research optimized the purification condition of total flavonoids crude extract (TFCE) of sea buckthorn peel residue using AB-8 macroporous resin by means of static and dynamic adsorption and desorption experiment. Ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-Q-MS) was used to analyze the component of TFSE. In addition, the inhibitory effect and types of TFSE on pancreatic lipase were explored through the method of nitrophenyl butyrate combined with enzymatic reaction kinetics. The results showed that the optimal purification conditions for AB-8 macroporous resin were a sample concentration of 2.2 mg/mL, a sample pH of 5.0, a sample volume of 140 mL, and a sample flow rate of 1.0 mL/min. The volume fraction of ethanol was 75% with the eluent being 120 mL and the elution flow rate being 1.5 mL/min. After separation and purification under these conditions, the purity of TFSE increased from 9.786% to 50.204%. The total of 39 phenolic compounds were identified from TFSE with flavonoids like rutin, catechins, and quercetin-3-O-glucoside being the main compounds. TFSE had a strong inhibition on pancreatic lipase with the IC50 being 12.46 mg/mL. In addition, the inhibition type was determined to be non-competitive inhibition by using double reciprocal plot method of Lineweaver-Burk with an inhibition constant Ki being 10.21 mg/mL. TFSE has better inhibitory activity on pancreatic lipase, which is of great significance for improving the added value of sea buckthorn peel residue products.

     

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