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中国精品科技期刊2020
赵猛,丁子康,李欣悦,等. 低分子量坛紫菜多糖纳米硒的制备、表征及其体外抗氧化活性[J]. 食品工业科技,2024,45(23):1−9. doi: 10.13386/j.issn1002-0306.2023120060.
引用本文: 赵猛,丁子康,李欣悦,等. 低分子量坛紫菜多糖纳米硒的制备、表征及其体外抗氧化活性[J]. 食品工业科技,2024,45(23):1−9. doi: 10.13386/j.issn1002-0306.2023120060.
ZHAO Meng, DING Zikang, LI Xinyue, et al. Preparation, Characterization and in Vitro Antioxidant Activity of Low Molecular Weight Porphyra haitanensis Polysaccharides Nano-selenium[J]. Science and Technology of Food Industry, 2024, 45(23): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120060.
Citation: ZHAO Meng, DING Zikang, LI Xinyue, et al. Preparation, Characterization and in Vitro Antioxidant Activity of Low Molecular Weight Porphyra haitanensis Polysaccharides Nano-selenium[J]. Science and Technology of Food Industry, 2024, 45(23): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120060.

低分子量坛紫菜多糖纳米硒的制备、表征及其体外抗氧化活性

Preparation, Characterization and in Vitro Antioxidant Activity of Low Molecular Weight Porphyra haitanensis Polysaccharides Nano-selenium

  • 摘要: 目的:制备低分子量坛紫菜多糖纳米硒(SPS-SeNPs),检测其形态特征及抗氧化活性。方法:以多糖浓度、反应时间、反应温度作为实验因素,采用双波长比色法测定SPS-SeNPs中纳米硒(SeNPs)的粒径变化,以SPS-SeNPs溶液的A410/490比值为评价指标,采用单因素及正交试验考察SPS-SeNPs的制备工艺。使用扫描电子显微镜、透射电子显微镜、X射线衍射和X射线光电子能谱分析对SPS-SeNPs进行结构表征。研究其总抗氧化活性、还原能力、DPPH自由基清除率。结果:制备SPS-SeNPs最佳条件为:多糖浓度2 mg/mL,反应温度为40 ℃,反应时间为0.5 h,该条件下制备出的SPS-SeNPs溶液吸光度比值为2.0487±0.0082。SPS-SeNPs中的SeNPs呈现出具有良好分散性的球形形态。确定了SPS-SeNPs的元素组成百分比为:C元素(42.00%)、O元素(41.35%)、Se元素(16.65%)。证实了SPS-SeNPs中的Se为0价态的无定形硒。通过稳定性考察发现SPS-SeNPs能在4 ℃避光保存30 d仍保持稳定。与亚硒酸钠(Na2SeO3)和低分子量坛紫菜多糖(SPS)相比,SPS-SeNPs表现出良好的总抗氧化能力、还原能力和DPPH自由基清除能力(IC50=31.45±1.16 µg/mL)。结论:SPS-SeNPs结合了多糖和纳米硒的优点,既具有多糖的抗氧化性,又具有纳米硒的高生物活性。这为进一步开发和利用坛紫菜粗多糖提供了重要的理论依据。

     

    Abstract: Objective: This study endeavors to synthesize selenium nanoparticles (SPS-SeNPs) by low molecular weight Porphyra haitanensis polysaccharide, with a primary focus on assessing their morphological attributes and antioxidant efficacy. Methods: Polysaccharide concentration, reaction time, and reaction temperature were systematically manipulated to optimize the preparation process. The preparation process of SPS-SeNPs was systematically investigated through both single-factor and orthogonal experiments. The determination of SeNP particle size within SPS-SeNPs was executed via dual-wavelength colorimetry, with the A410/490 ratio of the SPS-SeNPs solution serving as a pivotal evaluation metric. Structural elucidation encompassed scanning electron microscopy (SEM), transmission electron microscopy(TEM), X-ray diffraction(XRD), and X-ray photoelectron spectroscopy(XPS). The investigation extended to the assessment of total antioxidant activity, reducing power, and DPPH free radical scavenging rate. Results: The optimal conditions for SPS-SeNPs preparation were ascertained at a polysaccharide concentration of 2 mg/mL, a reaction temperature of 40 ℃, and a reaction time of 0.5 h, resulting in an absorbance ratio of 2.0487±0.0082. SeNPs within SPS-SeNPs exhibited a well-dispersed spherical morphology. The elemental composition percentages of SPS-SeNPs were found to be as follows: C (42%), O (41.35%), and Se (16.65%). The selenium within SPS-SeNPs was validated to be in an amorphous state with a zero valence, maintaining stability over 30 days at 4 ℃ under dark storage conditions. It demonstrated outstanding total antioxidant capacity, reducing power, and DPPH free radical scavenging ability (IC50=31.45±1.16 µg/mL), surpassing the performance of sodium selenite (Na2SeO3) and low molecular weight P. haitanensis polysaccharides (SPS). Conclusions: SPS-SeNPs synergistically harness the antioxidative properties of polysaccharides and the heighten biological activity of nano-selenium, thereby furnishing substantial theoretical underpinning for the progressive development and application of crude P. haitanensis polysaccharides.

     

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