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中国精品科技期刊2020
吕素媛,商冰清,孙璐颜,等. 微波辅助萃取油松松针精油的工艺优化及抗氧化活性研究[J]. 食品工业科技,2025,46(2):1−8. doi: 10.13386/j.issn1002-0306.2023120080.
引用本文: 吕素媛,商冰清,孙璐颜,等. 微波辅助萃取油松松针精油的工艺优化及抗氧化活性研究[J]. 食品工业科技,2025,46(2):1−8. doi: 10.13386/j.issn1002-0306.2023120080.
LÜ Suyuan, SHANG Bingqing, SUN Luyan, et al. Process Optimization and Antioxidant Activity of Pine Needle Essential Oil Extracted by Microwave-assisted Extraction[J]. Science and Technology of Food Industry, 2025, 46(2): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120080.
Citation: LÜ Suyuan, SHANG Bingqing, SUN Luyan, et al. Process Optimization and Antioxidant Activity of Pine Needle Essential Oil Extracted by Microwave-assisted Extraction[J]. Science and Technology of Food Industry, 2025, 46(2): 1−8. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120080.

微波辅助萃取油松松针精油的工艺优化及抗氧化活性研究

Process Optimization and Antioxidant Activity of Pine Needle Essential Oil Extracted by Microwave-assisted Extraction

  • 摘要: 目的:优化油松松针精油的最佳萃取工艺,并对油松松针精油进行成分分析、抗氧化研究。方法:响应面法优化微波辅助萃取(Microwave-assisted extraction,MAE)提取油松松针精油的工艺,利用气相色谱-质谱联用仪(Gaschromatography-mass spectrometry,GC-MS)对萃取的松针精油进行成分检测,并以DPPH、ABTS+自由基为指标探究其抗氧化活性。结果:最佳萃取工艺为:萃取时间55 min、微波功率550 W、液料比10:1,得率预测值0.245%,优化工艺验证结果为0.238%。从精油中得到的候选化合物共104种,其主要成分有桑贝醇(3.938%)、verticillol(3.597%)等。抗氧化实验中以L-抗坏血酸为对照,测定其DPPH和ABTS+自由基清除能力,其半抑制浓度(Half maximal inhibitory concentration,IC50)分别为101.74 mg/mL和12.39 mg/mL。松针精油浓度为128.00 mg/mL时,DPPH自由基清除率可达64.92%,ABTS+自由基清除率可达84.11%。结论:通过优化微波辅助萃取油松松针精油的工艺,并测定该方法获得的松针精油的主要挥发性成分,证实其抗氧化活性,为开发松针精油绿色高效提取方法及进一步利用松针精油资源提供了理论支持。

     

    Abstract: Objective: To optimize the best process for the extraction of essential oil from Pinus tabulatus, and to conduct it's compositional analysis and antioxidant capacity. Methods: Response surface methodology was used to optimize the process of extracting the essential oil from Pinus tabulatus by microwave-assisted extraction (MAE), the components of essential oil were identified by gas chromatography-mass spectrometry (GC-MS) and the antioxidant activity was verified by DPPH and ABTS+ free radicals as indicators. Results: The optimal extraction process were as follows: extraction time 55 min, microwave power 550 W, liquid-to-solid ratio 10:1 V/m, the predicted yield was 0.245%, and the validation result of the optimized process was 0.238%. A total of 104 candidate components were obtained from the essential oil, with major components including thunbergol (3.938%), and verticillol (3.597%), etc. In the antioxidant experiment, L-ascorbic acid was used as a control to determine the DPPH and ABTS+ radical scavenging ability, and the half effective concentration (IC50) was 101.74 mg/mL and 12.39 mg/mL, respectively. Meanwhile, at the concentration of essential oil of 128.00 mg/mL the DPPH radical scavenging rate could reach 64.92% and the ABTS+ radical scavenging rate could reach 84.11%. Conclusion: By optimizing the microwave-assisted extraction process of essential oil from Pinus tabulatus, and measuring the main volatile components of pine needle essential oil obtained by this method, its antioxidant activity was confirmed, which provided theoretical support for the development of green and efficient extraction methods of pine needle essential oil and further utilization of pine needle essential oil resources.

     

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