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中国精品科技期刊2020
王艳艳,肖兴宁,李建,等. 氮化碳与壳聚糖联合作用对沙门氏菌脂质代谢的影响[J]. 食品工业科技,2024,45(22):1−9. doi: 10.13386/j.issn1002-0306.2023120142.
引用本文: 王艳艳,肖兴宁,李建,等. 氮化碳与壳聚糖联合作用对沙门氏菌脂质代谢的影响[J]. 食品工业科技,2024,45(22):1−9. doi: 10.13386/j.issn1002-0306.2023120142.
WANG Yanyan, XIAO Xingning, LI Jian, et al. Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium[J]. Science and Technology of Food Industry, 2024, 45(22): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120142.
Citation: WANG Yanyan, XIAO Xingning, LI Jian, et al. Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium[J]. Science and Technology of Food Industry, 2024, 45(22): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120142.

氮化碳与壳聚糖联合作用对沙门氏菌脂质代谢的影响

Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium

  • 摘要: 目的:以鼠伤寒沙门氏菌为研究对象,从细菌脂质代谢层面探究氮化碳-壳聚糖复合溶胶作用下的杀菌机理。方法:基于透射电子显微镜和蛋白泄露分析氮化碳和壳聚糖联用对细菌细胞壁膜的破坏,并采用脂质代谢组,通过差异代谢物功能及其调控网络从细菌脂质代谢水平探究杀菌机理。结果:与单独使用氮化碳或壳聚糖相比,复合溶胶对鼠伤寒沙门氏菌细胞壁膜的损伤程度更大。脂质代谢中显著上调和下调的差异代谢物分别为203个和95个,主要集中在与细胞膜构成骨架相关的脂肪酸类、甘油磷脂类和糖脂类中。代谢物功能和调控网络分析表明,复合溶胶处理后,细菌脂肪酸生物合成、甘油磷脂代谢、亚油酸代谢、四烯酸代谢等代谢通路被破坏,细胞的能量代谢、物质运输和信号传导等生长代谢过程产生紊乱,进而导致细菌死亡。结论:本研究从脂质代谢角度揭示,氮化碳与壳聚糖联合破坏沙门氏菌的代谢通路,特别是与脂质代谢相关的通路,导致细菌代谢紊乱并死亡。这为光催化材料的杀菌机制提供了理论依据。

     

    Abstract: Objective: To investigate the bactericidal mechanism of a composite sol made of carbon nitride and chitosan on Salmonella typhimurium by studying its effects on lipid metabolism. Methods: Transmission electron microscopy and protein leakage concentration were utilized to evaluate the magnitude of cell membrane damage induced by carbon nitride-chitosan composite sol at a cellular level. In addition, lipid metabolomics was employed to investigate the bactericidal process by examining several metabolite functions and regulatory networks associated with bacterial lipid metabolism. Results: The composite sol of carbon nitride-chitosan caused more extensive damage to the cell membrane of Salmonella typhimurium. In lipid metabolism, the substantial up-regulated in 203 metabolites and down-regulated in 95 metabolites were observed. These metabolites were particularly involved in the metabolism of fatty acids, glycerophospholipids, and glycolipids, which play an important part in maintaining the integrity of cell membranes. Metabolite function analysis showed that the composite sol treatment disrupted various metabolic pathways in bacteria. These pathways include fatty acid biosynthesis, glycerol phospholipid metabolism, linoleic acid metabolism, tetraenoic acid metabolism, as well as pathways related to cell energy metabolism, material transport, and signal conduction. These disturbances finally resulted in the demise of the bacteria. Conclusion: This study reveals from the perspective of lipid metabolism that the combination of carbon nitride and chitosan disrupts the metabolic pathways of Salmonella, especially those related to lipid metabolism, leading to bacterial metabolic disruption and death. It provides a theoretical basis for comprehending the mechanisms by which photocatalytic materials exert their antibacterial properties.

     

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