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中国精品科技期刊2020
王鑫,课净璇,王亚斌,等. 提取方法对黄精渣水不溶性膳食纤维结构和功能特性的影响[J]. 食品工业科技,2024,45(24):196−205. doi: 10.13386/j.issn1002-0306.2024010181.
引用本文: 王鑫,课净璇,王亚斌,等. 提取方法对黄精渣水不溶性膳食纤维结构和功能特性的影响[J]. 食品工业科技,2024,45(24):196−205. doi: 10.13386/j.issn1002-0306.2024010181.
WANG Xin, KE Jingxuan, WANG Yabin, et al. Effects of Extraction Methods on Structural and Functional Properties of Water-insoluble Dietary Fiber from Polygonatum sibiricum Residue[J]. Science and Technology of Food Industry, 2024, 45(24): 196−205. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024010181.
Citation: WANG Xin, KE Jingxuan, WANG Yabin, et al. Effects of Extraction Methods on Structural and Functional Properties of Water-insoluble Dietary Fiber from Polygonatum sibiricum Residue[J]. Science and Technology of Food Industry, 2024, 45(24): 196−205. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024010181.

提取方法对黄精渣水不溶性膳食纤维结构和功能特性的影响

Effects of Extraction Methods on Structural and Functional Properties of Water-insoluble Dietary Fiber from Polygonatum sibiricum Residue

  • 摘要: 以黄精渣为原料,探究不同提取方法对黄精渣水不溶性膳食纤维(IDF)理化性质和功能特性的影响。采用复合酶法(CE)、超声辅助复酶法(UCE)和酶碱法(CEA)提取IDF,并考察三种IDF的组成、理化特性、葡萄糖吸附能力、阳离子交换能力和DPPH自由基清除能力等功能特性。研究表明,超声辅助复酶法得率最高(84.68%)。三种黄精渣IDF纯度均较高,主要由纤维素、半纤维素和木质素组成,其中UCE-IDF纯度高达82.24%。UCE-IDF持水力(4.74±0.66)g/g、持油力(3.84±0.29)g/g和结合水力(3.29±0.09)g/g最优,总酚含量(7.77±0.07)mg/g最高,阳离子交换能力(0.40±0.003)mmol/g最佳,抗氧化能力最佳。CEA-IDF的膨胀力(5.33±0.11)mL/g和总黄酮含量(1.88±0.03)mg/g最高。CE-IDF的葡萄糖吸附能力(19326.67±41.63)μmol/g最佳。理化表征显示,UCE-IDF结构更加不规则且具有更高的热稳定性。红外光谱显示三种黄精渣IDF均具有纤维素类特征吸收峰。X射线结果显示三种黄精渣IDF呈纤维素I晶型。综上,三种IDF的理化和功能特性之间存在差异,UCE-IDF在持水、持油等多项理化特性中具有明显优势,且热稳定性和抗氧化活性最佳。CEA-IDF的总黄酮含量最高且膨胀力最佳。CE-IDF的葡萄糖吸附能力最佳,本实验结果可为黄精渣IDF在食品加工中的应用提供理论参考。

     

    Abstract: The effects of different extraction methods on the physical and chemical properties and functional characteristics of insoluble dietary fiber (IDF) from Polygonatum sibiricum residue were investigated. The complex enzyme method (CE), ultrasonic-assisted complex enzyme method (UCE) and enzyme-alkali method (CEA) were used to extract IDF. The composition, physicochemical properties, glucose adsorption capacity, cation exchange capacity and DPPH free radical scavenging capacity of three IDFs were analyzed. Results showed that the yield of ultrasound-assisted compound enzyme method was the highest (84.68%). Three IDFs had a higher purity and mainly consisted of cellulose, hemicellulose, and lignin. The purity of UCE-IDF was up to 82.24%. UCE-IDF had the best water holding capacity (4.74±0.66) g/g, oil holding capacity (3.84±0.29) g/g, binding capacity (3.29±0.09) g/g, the highest total phenol content (7.77±0.07) mg/g, the best cation exchange capacity (0.40±0.003) mmol/g, and the antioxidant capacity. CEA-IDF had the highest swelling capacity (5.33±0.11) mL/g and flavonoid content (1.88±0.03) mg/g. CE-IDF had the best glucose adsorption capacity (19326.67±41.63) μmol/g. Physical and chemical characterization indicated that the structure of UCE-IDF was more irregular and had higher thermal stability. The infrared spectra suggested that the three IDFs had the characteristic absorption peaks of cellulose. The X-ray results revealed that the three IDFs were cellulose I crystal. In summary, there were differences in physical and chemical properties and functional characteristics among the three IDFs. UCE-IDF showed significant advantages in several physical and chemical properties, such as water holding, oil holding thermal stability, and antioxidant activity. CEA-IDF was distinguished by its high flavonoid content and the best swelling power. CE-IDF had the best glucose adsorption capacity. The results of this study could provide a theoretical basis for the application of IDF in food processing industry.

     

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