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中国精品科技期刊2020
何静静,杨晓宽. 板栗果酒多糖的提取纯化及其抗疲劳活性[J]. 食品工业科技,2024,45(24):232−242. doi: 10.13386/j.issn1002-0306.2024020031.
引用本文: 何静静,杨晓宽. 板栗果酒多糖的提取纯化及其抗疲劳活性[J]. 食品工业科技,2024,45(24):232−242. doi: 10.13386/j.issn1002-0306.2024020031.
HE Jingjing, YANG Xiaokuan. Extraction and Purification of Polysaccharide from Chestnut Fruit Wine and Its Anti-fatigue Activity[J]. Science and Technology of Food Industry, 2024, 45(24): 232−242. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020031.
Citation: HE Jingjing, YANG Xiaokuan. Extraction and Purification of Polysaccharide from Chestnut Fruit Wine and Its Anti-fatigue Activity[J]. Science and Technology of Food Industry, 2024, 45(24): 232−242. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020031.

板栗果酒多糖的提取纯化及其抗疲劳活性

Extraction and Purification of Polysaccharide from Chestnut Fruit Wine and Its Anti-fatigue Activity

  • 摘要: 目的:本研究对板栗果酒多糖进行了提取纯化和结构表征,并对其抗疲劳活性进行了研究。方法:采用超声波辅助水提醇沉法提取板栗果酒粗多糖,用超滤技术进行纯化分离。利用离子色谱、凝胶渗透色谱、紫外光谱和红外光谱等方法对其结构进行分析,并通过小鼠负重游泳实验来研究其抗疲劳的功效。结果:经超滤膜分离纯化得到板栗果酒多糖CFWP-1(>100 kU)和CFWP-2(<100 kU)。这两种多糖都属于非均一多糖,CFWP-1的重均分子量为132351 Da和664 Da,CFWP-2的重均分子量达133619 Da和664 Da。单糖分析结果表明,CFWP-1和CFWP-2主要由阿拉伯糖、半乳糖、葡萄糖、半乳糖醛酸、甘露糖醛酸5种单糖成分组成,且这两种多糖中葡萄糖含量均达98%以上,属于中性葡聚糖。紫外光谱表明,2种多糖的纯度较高。红外光谱分析显示,这两种多糖都有多糖典型的吸收峰。抗疲劳试验表明,CFWP-1和CFWP-2均能显著提高小鼠血清中的超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)(P<0.05)的活力,并能明显地降低乳酸脱氢酶(Lactate dehydrogenase,LDH)、血清尿素氮(Serum urea nitrogen,BUN)、乳酸(Blood lactate,BLA)、丙二醛(malondialdehyde,MDA)和肌酸激酶(creatine kinase,CK)的水平(P<0.05),可以显著提升糖原储备水平,对小鼠骨骼肌具有良好的保护作用,且CFWP-2的效果优于CFWP-1,并呈剂量依赖性。结论:从板栗果酒中提取出的多糖具有较好的抗疲劳作用。

     

    Abstract: : Objective: In this study, the polysaccharide of chestnut fruit wine was extracted, purified and characterized, and its anti-fatigue activity was studied. Methods: The crude polysaccharide of chestnut fruit wine was extracted by ultrasonic-assisted water extraction and alcohol precipitation, and purified and separated by ultrafiltration technology. Its structure was analyzed by ion chromatography, gel permeation chromatography, ultraviolet spectroscopy and infrared spectroscopy, and its anti-fatigue effect was studied by weight-bearing swimming test in mice. Results: CFWP-1(> 100 kU) and CFWP-2 (< 100 kU) were isolated and purified by ultrafiltration membrane. Both of these polysaccharides are heterogeneous, with the average molecular weight of CFWP-1 being 132351 Da and 664 Da, and the average molecular weight of CFWP-2 being 133619 Da and 664 Da. The results of monosaccharide analysis showed that CFWP-1 and CFWP-2 were mainly composed of arabinose, galactose, glucose, galacturonic acid and mannouronic acid, and the glucose content in these two polysaccharides was more than 98%, which belonged to neutral glucan. UV spectra showed that the purity of the two polysaccharides was high. Infrared spectroscopy analysis showed that both polysaccharides had typical absorption peaks of polysaccharides. Anti-fatigue experiments showed that both CFWP-1 and CFWP-2 could significantly increase the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum of mice (P<0.05). The levels of lactate dehydrogenase (LDH), serum urea nitrogen (BUN), blood lactate (BLA), malondialdehyde (MDA) and creatine kinase (CK) were significantly decreased (P<0.05), and the glycogen storage level was significantly increased. It has a good protective effect on skeletal muscle in mice. The effect of CFWP-2 was better than that of CFWP-1 in a dose-dependent manner. Conclusion: The polysaccharide extracted from chestnut fruit wine has good anti-fatigue effect.

     

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