• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农林核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
陆源添,刘迪. 杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析[J]. 食品工业科技,2025,46(2):1−10. doi: 10.13386/j.issn1002-0306.2024020046.
引用本文: 陆源添,刘迪. 杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析[J]. 食品工业科技,2025,46(2):1−10. doi: 10.13386/j.issn1002-0306.2024020046.
LU Yuantian, LIU Di. Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus[J]. Science and Technology of Food Industry, 2025, 46(2): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020046.
Citation: LU Yuantian, LIU Di. Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus[J]. Science and Technology of Food Industry, 2025, 46(2): 1−10. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020046.

杨树桑黄与紫孢侧耳共培养胞内多糖提取工艺优化及抗氧化活性分析

Optimization of Intracellular Polysaccharide Extraction Process and Analysis of Antioxidant Activity in Co-cultures of Sanghuangporus vaninii and Pleurotus sapidus

  • 摘要: 本研究旨在探究超声波协同半仿生法提取杨树桑黄与紫孢侧耳液体共培养胞内多糖(CC-IPS)的工艺。通过单因素实验、Plackett-Burman设计和响应面设计试验,探究第1、2、3次的提取料液比、提取时间、提取温度和提取液pH这12个因素对CC-IPS提取量的影响,以DPPH和ABTS+自由基清除率评价CC-IPS的体外抗氧化能力。结果表明:最佳提取工艺参数为第1、2、3次提取料液比分别为1:17、1:40、1:30(g/mL),第1、2、3次提取时间分别为31、30、30 min,第1、2、3次提取温度分别为44、40、50 ℃,第1、2、3次提取液pH分别为3、8、10。在此最优工艺条件下,CC-IPS提取量为86.4921±1.1924 mg/g,与预测值85.3981 mg/g接近。CC-IPS对DPPH和ABTS+自由基清除率的IC50分别为0.2425 mg/mL和0.1376 mg/mL。该研究为真菌液体共培养胞内多糖的综合高效利用开发提供理论基础。

     

    Abstract: In this study, we explored an ultrasonic synergistic semi-bionic method to extract intracellular polysaccharides from Sanghuangporus vaninii and Pleurotus sapidus liquid co-cultures. By conducting single-factor experiments, Plackett-Burman design, and response surface design, we investigated the impact of 12 factors on co-culture intracellular polysaccharide (CC-IPS) extraction content, including extraction material-liquid ratios, extraction time, extraction temperature, and extraction solution pH. The antioxidant capacity of CC-IPS in vitro was evaluated by the DPPH and ABTS+ radical scavenging rates. The optimal extraction process parameters for the first, second, and third extractions, respectively, included material-to-liquid ratios of 1:17, 1:40, and 1:30 (g/mL), extraction times of 31, 30, and 30 min, extraction temperatures of 44, 40, and 50 ℃, and extraction solution pH levels of 3, 8, and 10. Under these optimal conditions, the CC-IPS extraction content was 86.4921±1.1924 mg/g, close to the predicted value of 85.3981 mg/g. Additionally, the IC50 values of CC-IPS in the DPPH and ABTS+ free radical scavenging assays were determined to be 0.2425 and 0.1376 mg/mL, respectively. This study offers a theoretical foundation for the efficient utilization of intracellular polysaccharides in liquid fungal co-cultures.

     

/

返回文章
返回