• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
吴慧昊, 牛锋, 钟琦, 杨迪. 高效降胆固醇降甘油三酯乳酸菌的分离鉴定和功能分析[J]. 食品工业科技, 2019, 40(14): 157-162. DOI: 10.13386/j.issn1002-0306.2019.14.026
引用本文: 吴慧昊, 牛锋, 钟琦, 杨迪. 高效降胆固醇降甘油三酯乳酸菌的分离鉴定和功能分析[J]. 食品工业科技, 2019, 40(14): 157-162. DOI: 10.13386/j.issn1002-0306.2019.14.026
WU Hui-hao, NIU Feng, ZHONG Qi, YANG Di. Separation, Identification and Functional Analysis of Cholesterol-lowering and Triglycerid-lowering Lactic Acid Bacteria[J]. Science and Technology of Food Industry, 2019, 40(14): 157-162. DOI: 10.13386/j.issn1002-0306.2019.14.026
Citation: WU Hui-hao, NIU Feng, ZHONG Qi, YANG Di. Separation, Identification and Functional Analysis of Cholesterol-lowering and Triglycerid-lowering Lactic Acid Bacteria[J]. Science and Technology of Food Industry, 2019, 40(14): 157-162. DOI: 10.13386/j.issn1002-0306.2019.14.026

高效降胆固醇降甘油三酯乳酸菌的分离鉴定和功能分析

Separation, Identification and Functional Analysis of Cholesterol-lowering and Triglycerid-lowering Lactic Acid Bacteria

  • 摘要: 筛选出高效降胆固醇降甘油三酯(Triglyceride,TG)菌株,为新型药物、保健食品的研制提供优良菌株。从6种西北特有样品中筛选分离出乳酸菌,采用磷硫铁比色法测定菌株降胆固醇能力,并测定菌株降甘油三酯能力,通过抑菌实验、耐酸、耐胆盐及小鼠体内毒性试验研究菌株各项性能,利用生理生化及16S rDNA法对菌株进行鉴定。结果表明,本试验筛选出3株高效降胆固醇降甘油三酯乳酸菌,其中菌株ZL010降胆固醇、降甘油三酯效果最强,蛋黄中胆固醇的降解率为73.65%,高纯度胆固醇降解率为40.06%,甘油三酯降解率为5.10%。3株菌都具有很好的抑菌、耐酸、耐胆盐能力,菌株经生理生化及16S rDNA鉴定ZL005为耐久肠球菌(Enterococcus durans),ZL010为植物乳杆菌(Lactobacillus plantarum),ZL016为戊糖乳杆菌(Lactobacillus pentosus)。小鼠毒性试验表明,菌株无毒副作用,小鼠各脏器一切正常,菌株可用于降脂降胆固醇保健药品及功能食品的制备。

     

    Abstract: In order to provide excellent strains for the preparation of new drugs and food, the cholesterol-lowering and triglyceride-lowering lactic acid bacteria were screened. Lactic acid bacteria were isolated from six samples of northwest endemic species. Triglyceride degradation and cholesterol-lowering abilities were determined by phosphorus-sulfur-iron colorimetry method. The bacteriostatic test, acid and bile salt tolerance test and toxicity test in mice were used to study the properties of the strain. The strain was identified by physiological and biochemical methods and 16S rDNA. The results showed that there were 3 cholesterol-lowering and triglyceride-lowering lactic acid bacteria. The strain ZL010 had the strongest effect that the egg yolk cholesterol degradation rate was 73.65%, the high purity cholesterol degradation rate was 40.06%, the triglyceride degradation rate was 5.1%. The 3 strains had good bacteriostatic ability, and showed strong tolerance to acid and bile salt. The 16S rDNA identified strain ZL005, ZL010, ZL016 as Enterococcus durans, Lactobacillus plantarum, Lactobacillus pentosus. The toxicity test revealed the strain has no toxic and side effects, the mice were dissected and observed that all the organs were normal. The strains could be used in the preparation of lipid-lowering and cholesterol-lowering drugs and functional foods.

     

/

返回文章
返回