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中国精品科技期刊2020
耿多, 陈辉, 罗瑞明, 王丽娟. 基于基因检测方法快速鉴别不同种类畜肉[J]. 食品工业科技, 2019, 40(15): 66-71,187. DOI: 10.13386/j.issn1002-0306.2019.15.011
引用本文: 耿多, 陈辉, 罗瑞明, 王丽娟. 基于基因检测方法快速鉴别不同种类畜肉[J]. 食品工业科技, 2019, 40(15): 66-71,187. DOI: 10.13386/j.issn1002-0306.2019.15.011
GENG Duo, CHEN Hui, LUO Rui-ming, WANG Li-juan. Rapid Identification of Different Animal Meat Based on Gene Detection[J]. Science and Technology of Food Industry, 2019, 40(15): 66-71,187. DOI: 10.13386/j.issn1002-0306.2019.15.011
Citation: GENG Duo, CHEN Hui, LUO Rui-ming, WANG Li-juan. Rapid Identification of Different Animal Meat Based on Gene Detection[J]. Science and Technology of Food Industry, 2019, 40(15): 66-71,187. DOI: 10.13386/j.issn1002-0306.2019.15.011

基于基因检测方法快速鉴别不同种类畜肉

Rapid Identification of Different Animal Meat Based on Gene Detection

  • 摘要: 本研究以牛、羊、猪、狗、马5种畜肉为研究对象,建立基因快速检测方法。针对牛、羊、猪、狗、马线粒体基因筛选特异性引物,优化各畜种检测条件。利用特异性引物扩增各畜种DNA,制备重组质粒测序,建立常见肉及肉制品快速检测模型。结果表明,此方法成功实现了5种畜肉在30 min内同时快速检测,各畜种核酸引物特异性强,与其它物种无交叉反应发生。重组质粒测序结果与靶基因序列比对,同源性达100%。畜肉基因最低检测限为5×10-7 μg,表明该快速检测方法特异性强、灵敏度高,可同时检测多个畜肉样品,完全适用于熟肉样品的检测,为快速鉴别牛、羊、猪、狗、马多畜种畜肉提供依据,并为以快速鉴别不同畜种为前提开展的研究提供理论参考。

     

    Abstract: In this paper,five breeds of cattle,sheep,pig,dog and horse were taken as subject,a method for rapid detection and identification of the genes was established. Specific primers were screened for the mitochondrial genes of cattle,sheep,pigs,dogs and horses,and the conditions for detection of all breeds were optimized. Specific primers were used to amplify the DNA of each breed,and the recombinant plasmids were sequenced to establish a rapid detection model for common meat and meat products. The results showed that this method could successfully detect five kinds of breeds in 30 minutes at the same time. The specificity of nucleic acid primers was strong,and there was no cross reaction with other species.The sequence of recombinant plasmid was aligned with the sequence of target gene,and the homology was 100%. The minimum detection limit of meat gene was 5×10-7 μg,indicating that the rapid detection method was highly specific and sensitive. The method could detect several breed samples simultaneously,and was suitable for the detection of cooked meat samples. It provided a basis for quick identification of cattle,sheep,pigs,dogs and horses,and provided a theoretical reference for the rapid identification of different livestock species.

     

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