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中国精品科技期刊2020
张妍, 肖明, 张爽, 谢凤英, 张兰威. 保加利亚乳杆菌细胞壁蛋白酶基因克隆及结构分析[J]. 食品工业科技, 2019, 40(20): 107-111,119. DOI: 10.13386/j.issn1002-0306.2019.20.018
引用本文: 张妍, 肖明, 张爽, 谢凤英, 张兰威. 保加利亚乳杆菌细胞壁蛋白酶基因克隆及结构分析[J]. 食品工业科技, 2019, 40(20): 107-111,119. DOI: 10.13386/j.issn1002-0306.2019.20.018
ZHANG Yan, XIAO Ming, ZHANG Shuang, XIE Feng-ying, ZHANG Lan-wei. Gene Cloning and Structural Analysis of Cell Wall Proteinase from Lactobacillus bulgaricus[J]. Science and Technology of Food Industry, 2019, 40(20): 107-111,119. DOI: 10.13386/j.issn1002-0306.2019.20.018
Citation: ZHANG Yan, XIAO Ming, ZHANG Shuang, XIE Feng-ying, ZHANG Lan-wei. Gene Cloning and Structural Analysis of Cell Wall Proteinase from Lactobacillus bulgaricus[J]. Science and Technology of Food Industry, 2019, 40(20): 107-111,119. DOI: 10.13386/j.issn1002-0306.2019.20.018

保加利亚乳杆菌细胞壁蛋白酶基因克隆及结构分析

Gene Cloning and Structural Analysis of Cell Wall Proteinase from Lactobacillus bulgaricus

  • 摘要: 本文以保加利亚乳杆菌基因组为模板,利用PCR的方法扩增细胞壁蛋白酶基因(prtB),并且利用GeneBank数据库和生物软件对prtB进行对比和生物信息学分析,预测基因片段的相似性、表达出蛋白酶的蛋白大小、二级结构和膜结合性等性质。结果表明,测序后比对发现扩增片段与模板保加利亚乳杆菌prtB相似性为100%,证明基因片段未发生突变;prtB与德氏乳杆菌德氏亚种基因相似性为96%,与德氏乳杆菌乳酸亚种(Lactobacillus delbrueckii)基因相似性为82%,具有一定的保守性和特异性。prtB基因G+C含量为47.49%,A+T含量为52.51%,编码1831个氨基酸,分子量为3338.7 kD,将此蛋白命名为PrtB,等电点pI为8.76。保加利亚乳杆菌的PrtB为亲水性稳定蛋白,总平均亲水性为-0.583,有28个Ser、9个Thr和25个Tyr可能成为蛋白激酶磷酸化的作用位点,该蛋白为细胞外蛋白,其二级结构以不规则卷曲为主,其值为55.11%,α-螺旋和β-折叠仅占21.30%和23.59%。

     

    Abstract: The cell wall proteinase gene(prtB)was amplified by PCR using the Lactobacillus bulgaricus genome as a template,and the comparison and bioinformatics analysis of prtB were performed using GeneBank database and biological software to predict the properties of gene fragments,such as similarity,expression,protein size,secondary structure and biofilm binding. Results showed that,it was 100% similar to the prtB of template Lactobacillus bulgaricus after sequencing,which proved that the gene fragment did not undergo mutation. prtB showed 96% identity with Lactobacillus delbrueckii subsp. delbrueckii gene,and 82% identity with Lactobacillus delbrueckii subsp. lactis,which indicated the fragment had certain conservatism and specificity. The molecular weight of prtB was 3338.7 kD,and the contents of G+C and A+T were 47.49% and 52.51%. The prtB encoded 1831 amino acids,the protein of which was named PrtB,with isoelectric point pI=8.76. The protein PrtB as a hydrophilic stable protein had a total average hydrophilicity of-0.583,28 Ser,9 Thr and 25 Tyr as the possible site of protein kinase phosphorylation,which should be extracellular protein. The secondary structure of PrtB was mainly composed of irregular curl up to 55.11%,21.30% alpha helix and 23.59% beta fold.

     

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