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中国精品科技期刊2020
李盛楠, 张敏婕, 胡浩鑫, 冯沛彦, 曾静, 韦晓群. 高效液相色谱法测定液态乳中乳果糖方法的优化[J]. 食品工业科技, 2021, 42(1): 266-270. DOI: 10.13386/j.issn1002-0306.2020030194
引用本文: 李盛楠, 张敏婕, 胡浩鑫, 冯沛彦, 曾静, 韦晓群. 高效液相色谱法测定液态乳中乳果糖方法的优化[J]. 食品工业科技, 2021, 42(1): 266-270. DOI: 10.13386/j.issn1002-0306.2020030194
LI Sheng-nan, ZHANG Min-jie, HU Hao-xin, FENG Pei-yan, ZENG Jing, WEI Xiao-qun. Optimization of HPLC Method for Determination of Lactulose in Liquid Milk[J]. Science and Technology of Food Industry, 2021, 42(1): 266-270. DOI: 10.13386/j.issn1002-0306.2020030194
Citation: LI Sheng-nan, ZHANG Min-jie, HU Hao-xin, FENG Pei-yan, ZENG Jing, WEI Xiao-qun. Optimization of HPLC Method for Determination of Lactulose in Liquid Milk[J]. Science and Technology of Food Industry, 2021, 42(1): 266-270. DOI: 10.13386/j.issn1002-0306.2020030194

高效液相色谱法测定液态乳中乳果糖方法的优化

Optimization of HPLC Method for Determination of Lactulose in Liquid Milk

  • 摘要: 为了对液态乳中乳果糖含量进行简便准确地定量,改进高效液相色谱法测定乳果糖含量的方法。采用化学稳定的改性酰胺基色谱柱进行分离,并以乙腈-水流动相梯度洗脱40 min,结果表明,样品进样体积为3 μL时乳糖与乳果糖的分离度为1.5,满足色谱分离检测。优化后的方法经验证,乳果糖在50~2500 mg/kg范围内线性关系良好,(R2=0.9997),乳果糖检出限和定量限分别为15和50 mg/kg,3个加标水平(n=6)乳果糖平均回收率在90.3%~105.6%之间,相对标准偏差为3.4%~3.9%。利用该法对市场中的27种实际乳样进行分析,结果均在合理范围之内。该方法经济、准确适用于液态乳中乳果糖含量的日常检测。

     

    Abstract: In order to quantify the content of lactulose in liquid milk easily and accurately,the HPLC method for determination of lactulose in liquid milk was improved. The method used stable modified amide-based chromatographic column for separation,and eluted with acetonitrile-water mobile phase for 40 minutes gradiently. The results indicated that when the injection volume was 3 μL,the resolution was 1.5,which met the chromatographic separation detection. The optimized method has been verified that lactulose has a good linear relationship in the range of 50~2500 mg/kg,(R2=0.9997),the detection limit and the quantitative limit of lactulose were 15 mg/kg and 50 mg/kg respectively. The spiked recoveries of lactulose were in the range of 90.3%~105.6%,and relative standard deviations(n=6)in the range of 3.4%~3.9%. The proposed method was used to detect 27 actual milk samples,and the results were within a reasonable range. This method was economical and accurate for the daily detection of lactulose in liquid milk.

     

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