高效液相色谱法测定液态乳中乳果糖方法的优化

李盛楠; 张敏婕; 胡浩鑫; 冯沛彦; 曾静; 韦晓群

doi:10.13386/j.issn1002-0306.2020030194

高效液相色谱法测定液态乳中乳果糖方法的优化

doi: 10.13386/j.issn1002-0306.2020030194

1. 华南农业大学食品学院, 广东广州 210095;
2. 温氏食品集团股份有限公司, 广东新兴 527400;
3. 广州广电计量检测股份有限公司, 广东广州 510656

基金项目:

广州市科技计划资助项目（201803020044）。

详细信息

作者简介:
李盛楠(1994-),女,硕士研究生,研究方向:食品品质检测,E-mail:1132779059@qq.com。

通讯作者:
韦晓群(1960-),男,博士,副教授,研究方向:食品品质检测分析,E-mail:weixqun@scau.edu.cn。

中图分类号: TS207.3
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出版历程
- 收稿日期: 2020-03-18

Optimization of HPLC Method for Determination of Lactulose in Liquid Milk

1. College of Food Science, South China Agricultural University, Guangzhou 210095, China;
2. Wen's Food Group Co. Ltd., Xinxing 527400, China;
3. Guangzhou Radio and Television Metrology&Testing Co. Ltd., Guangzhou 510656, China

摘要

摘要: 为了对液态乳中乳果糖含量进行简便准确地定量，改进高效液相色谱法测定乳果糖含量的方法。采用化学稳定的改性酰胺基色谱柱进行分离，并以乙腈-水流动相梯度洗脱40 min，结果表明，样品进样体积为3 μL时乳糖与乳果糖的分离度为1.5，满足色谱分离检测。优化后的方法经验证，乳果糖在50~2500 mg/kg范围内线性关系良好，（R²=0.9997），乳果糖检出限和定量限分别为15和50 mg/kg，3个加标水平（n=6）乳果糖平均回收率在90.3%~105.6%之间，相对标准偏差为3.4%~3.9%。利用该法对市场中的27种实际乳样进行分析，结果均在合理范围之内。该方法经济、准确适用于液态乳中乳果糖含量的日常检测。
- 乳果糖 /
- 高效液相色谱 /
- 改性酰胺基色谱柱 /
- 液态乳
Abstract: In order to quantify the content of lactulose in liquid milk easily and accurately,the HPLC method for determination of lactulose in liquid milk was improved. The method used stable modified amide-based chromatographic column for separation,and eluted with acetonitrile-water mobile phase for 40 minutes gradiently. The results indicated that when the injection volume was 3 μL,the resolution was 1.5,which met the chromatographic separation detection. The optimized method has been verified that lactulose has a good linear relationship in the range of 50~2500 mg/kg,(R²=0.9997),the detection limit and the quantitative limit of lactulose were 15 mg/kg and 50 mg/kg respectively. The spiked recoveries of lactulose were in the range of 90.3%~105.6%,and relative standard deviations(n=6)in the range of 3.4%~3.9%. The proposed method was used to detect 27 actual milk samples,and the results were within a reasonable range. This method was economical and accurate for the daily detection of lactulose in liquid milk.
- lactulose /
- HPLC /
- modified X-amide column /
- liquid milk

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