沙棘多酚提取纯化工艺研究

吕佳玮; 王颉; 刘亚琼; 孙剑锋; 王文秀; 龚慧; 牟建楼

doi:10.13386/j.issn1002-0306.2020030331

沙棘多酚提取纯化工艺研究

doi: 10.13386/j.issn1002-0306.2020030331

河北农业大学食品科技学院, 河北保定 071000

基金项目:

河北省农产品加工工程技术研究中心运行绩效后补经费（199676183H）。

详细信息

作者简介:
吕佳玮(1995-),女,硕士研究生,研究方向:食品加工,E-mail:767602213@qq.com。

通讯作者:
牟建楼(1973-),女,硕士,副教授,研究方向:农产品加工及贮藏,E-mail:jianloumu2005@sina.cn。

中图分类号: TS255.1
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- 被引次数: 0
出版历程
- 收稿日期: 2020-03-26

Study on Extraction and Purification Process of Sea buckthorn Polyphenols

College of Food Science and Technology, Agricultural University of Hebei Province, Baoding 071000, China

摘要

摘要: 以沙棘冻干粉为原料，采用超声辅助乙醇法提取沙棘多酚，通过单因素试验和响应面试验优化提取条件，并通过大孔树脂对沙棘多酚进行静态吸附-解析试验，选取适合沙棘多酚纯化的大孔树脂，对纯化前后的沙棘多酚进行体外抗氧化试验。结果表明：沙棘多酚的最佳提取工艺为：料液比1∶24 g/mL，乙醇浓度49%，超声时间52 min，超声温度48℃，在此条件下沙棘多酚的提取量为8.61 mgECGC/g；确定AB-8为纯化沙棘多酚适合的大孔树脂，纯化前后清除DPPH自由基的IC₅₀值分别713.22、142.53 μg/mL，纯化前后清除ABTS自由基的IC₅₀值分别为61.92、8.68 μg/mL，经过纯化后的沙棘多酚抗氧化性明显升高。
- 沙棘 /
- 多酚 /
- 响应面 /
- 纯化 /
- 抗氧化性
Abstract: The raw material was sea buckthorn lyophilized powder,and the ultrasonic-assisted and ethanol solvent method was used to extract sea buckthorn polyphenols. The extraction conditions were optimized by single-factor test and response surface test. The macroporous resin was used for static adsorption-analysis test of sea buckthorn polyphenols. In vitro antioxidant tests were carried out on sea buckthorn polyphenols before and after purification. The test results showed that when the ratio of liquid to solid was 1∶24 (g/mL),the volume fraction of ethanol was 49%,the extraction time was 52 min,and the extraction temperature was 48 ℃,the extraction amount of polyphenol was the largest,reaching(8.61±0.25)mgECGC/g. The AB-8 was identified as suitable macroporous resin for purification of sea buckthorn polyphenols. The semi-inhibitory concentrations(IC₅₀)of polyphenols from sea buckthorn on DPPH free radical before and after purification were 713.22 and 142.53 μg/mL,and The semi-inhibitory concentrations(IC₅₀)of polyphenols from sea buckthorn on ABTS free radical before and after purification were 61.92,8.68 μg/mL. The results showed that the polyphenols from sea buckthorn after purification had good antioxidant capacity.
- sea buckthorn /
- polyphenols /
- response surface /
- purification /
- antioxidant

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参考文献(28)

[1]	张晓凤,杨永晶,薛延团,等.沙棘甾醇对大鼠急性乙醇型胃黏膜损伤的保护作用研究[J].中国食品添加剂,2019(11):125-131.
[2]	丁健,关莹,阮成江,等.沙棘果油提取工艺的正交试验优化及其脂肪酸组分测定[J].食品科学,2016,37(2):13-18.
[3]	郭瑞雪.沙棘酚类物质生物活性、生物利用度及其体内外抑制乳腺癌细胞增殖的机理研究[D].广州:华南理工大学,2019.
[4]	白生文,汤超,田京,等.沙棘果渣总黄酮提取工艺及抗氧化活性分析[J].食品科学,2015,36(10):59-64.
[5]	吕恒慧.沙棘果中活性物质的提取及其对心脏保护作用的研究[D].济南:山东师范大学,2016.
[6]	Kashyap P,Kumar S,Singh D.Performance of antifreeze protein HrCHI₄ from Hippophae rhamnoides in improving the structure and freshness of green beans upon cryopreservation[J]. Food Chemistry,2020,320:126599.
[7]	Shi L K,Zheng L,Zhao C W,et al. Chemical composition and antioxidant capacity of extracts from the whole berry,pulp and seed of Hippophae rhamnoides ssp.Yunnanensis[J].Natural Product Research,2019,33(24):3596-3600.
[8]	Dzah C S,Duan Y Q,Zhang H H,et al. The effects of ultrasound assisted extraction on yield,antioxidant,anticancer and antimicrobial activity of polyphenol extracts:a review[J].Food Bioscience,2020,35:100547.
[9]	Iglesias-Carres L,Mas-Capdevila A,Bravo F I,et al. Optimization of a polyphenol extraction method for sweet orange pulp(Citrus sinensis L.)to identify phenolic compounds consumed from sweet oranges[J].PLoS One,2019,14(1):e0211267.
[10]	杨新,陈莉,卢红梅,等.茶多酚提取与纯化方法及其功能活性研究进展[J].食品工业科技,2019,40(5):322-328 ,332.
[11]	孙伟鹏,马娜,党艳艳.沙棘果渣中多种有效成分的提取及其抗氧化性能研究[J].食品工业,2018,39(6):151-155.
[12]	Wei E W,Yang R,Zhao H P,et al. Microwave-assisted extraction releases the antioxidant polysaccharides from seabuckthorn(Hippophae rhamnoides L.)berries[J].International Journal of Biological Macromolecules,2019,123:280-290.
[13]	谢佳函,刘回民,刘美宏,等.红豆皮多酚提取工艺优化及抗氧化活性分析[J].中国食品学报,2020,20(1):147-157.
[14]	苏海兰,魏娟,毕阳,等.超声波辅助提取中国沙棘浆果多酚的工艺优化及其成分分析[J].食品与发酵科技,2017,53(6):34-41.
[15]	何婷,王凯,赵雷,等.大孔树脂纯化龙眼核多酚及其组分分析[J].食品工业科技,2019,40(16):1-6 ,13.
[16]	杨美莲,程桂广,蔡圣宝,等.朝鲜蓟叶多酚提取及抗氧化活性研究[J].现代食品科技,2019,35(4):157-165 ,21.
[17]	邵佩,陈胜,罗威,等.猕猴桃多酚的纯化工艺及组分分析[J].食品科技,2019,44(12):260-265.
[18]	García-Moreno P J,Batista I,Pires C,et al. Antioxidant activity of protein hydrolysates obtained from discarded Mediterranean fish species[J].Food Research International,2014,65:469-476.
[19]	姚宏亮,沈洁,佴逸凡.藕皮多酚提取工艺优化及其体外抗氧化性研究[J].食品研究与开发,2019,40(14):103-108.
[20]	胡会刚,赵巧丽.菠萝皮渣多酚的提取分离及其抗氧化活性评价[J].食品科技,2020,45(1):286-293.
[21]	Xu J L,Wang W C,Liang H,et al. Optimization of ionic liquid based ultrasonic assisted extraction of antioxidant compounds from Curcuma longa L. using response surface methodology[J].Industrial Crops and Products,2015,76:487-493.
[22]	戴倩倩,樊雨梅,张晓旭,等.朝鲜蓟茎叶副产物青贮过程中多酚及其功能活性的研究[J].中国食品学报,2020,20(2):263-270.
[23]	李佳运,李楠.基于超声波辅助的燕麦多酚提取及稳定性研究[J].食品工程,2019(4):16-20.
[24]	侯学敏,李林霞,张直峰,等.响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J].食品科学,2013,34(6):124-128.
[25]	巫永华,张建萍,赵节昌,等.大孔树脂纯化黄精多酚及其抗氧化性与组成分析[J].农业工程学报,2020,36(1):318-326.
[26]	卜凡琼,代雨婷,冯武,等.徐香猕猴桃多酚抗氧化特性研究[J].华中农业大学学报,2019,38(1):112-118.
[27]	李丽丽.柠檬桉树脂有效成分的提取、纯化及抗氧化活性研究[D].南宁:广西大学,2019.
[28]	蓝梧涛,吴雪辉,章文,等.油茶叶多酚的提取工艺优化及其体外抗氧化性的研究[J].中国油脂,2019,44(2):118-122.