菱角梗、菱角壳提取物抑菌活性及其活性成分分析

王洪斌 陈云舒 严守雷 梅大佐 赵道华 张剑雄 王清章 李洁

王洪斌, 陈云舒, 严守雷, 梅大佐, 赵道华, 张剑雄, 王清章, 李洁. 菱角梗、菱角壳提取物抑菌活性及其活性成分分析[J]. 食品工业科技, 2021, 42(3): 61-67. doi: 10.13386/j.issn1002-0306.2020040081
引用本文: 王洪斌, 陈云舒, 严守雷, 梅大佐, 赵道华, 张剑雄, 王清章, 李洁. 菱角梗、菱角壳提取物抑菌活性及其活性成分分析[J]. 食品工业科技, 2021, 42(3): 61-67. doi: 10.13386/j.issn1002-0306.2020040081
WANG Hongbin, CHEN Yunshu, YAN Shoulei, MEI Dazuo, ZHAO Daohua, ZHANG Jianxiong, WANG Qingzhang, LI Jie. Antibacterial Activity and Component Analysis of Water Chestnut Stem and Water Chestnut Pericarp Extracts[J]. Science and Technology of Food Industry, 2021, 42(3): 61-67. doi: 10.13386/j.issn1002-0306.2020040081
Citation: WANG Hongbin, CHEN Yunshu, YAN Shoulei, MEI Dazuo, ZHAO Daohua, ZHANG Jianxiong, WANG Qingzhang, LI Jie. Antibacterial Activity and Component Analysis of Water Chestnut Stem and Water Chestnut Pericarp Extracts[J]. Science and Technology of Food Industry, 2021, 42(3): 61-67. doi: 10.13386/j.issn1002-0306.2020040081

菱角梗、菱角壳提取物抑菌活性及其活性成分分析

doi: 10.13386/j.issn1002-0306.2020040081
基金项目: 

湖北省技术创新专项(2019ABA108)。

详细信息
    作者简介:

    王洪斌(1987-),男,硕士,讲师,研究方向:农产品保鲜加工与功能性成分,E-mail:whb879694@yeah.net。

    通讯作者:

    严守雷(1975-),男,博士,副教授,研究方向:水生蔬菜加工与贮藏,E-mail:yanshoulei1225@163.com。

  • 中图分类号: TS255.1

Antibacterial Activity and Component Analysis of Water Chestnut Stem and Water Chestnut Pericarp Extracts

  • 摘要: 采用滤纸片扩散法研究菱角梗、菱角壳乙醇提取液及其不同溶剂萃取物的抑菌活性,并考察不同温度、pH、紫外辐照对菱角梗、菱角壳乙酸乙酯萃取物抑菌活性稳定性的影响,最后,采用高效液相色谱法和超高效液质联用法对菱角梗、菱角壳乙酸乙酯萃取物中的成分进行鉴定。结果表明:菱角梗、菱角壳乙酸乙酯萃取物对金黄色葡萄球菌、大肠杆菌和酿酒酵母菌的最小抑菌浓度分别为0.391、0.391、3.125 mg/mL和0.098、0.195、1.563 mg/mL,抑菌效果显示,菱角壳乙酸乙酯萃取物优于菱角梗乙酸乙酯萃取物,二者萃取物均对革兰氏阳性菌具有显著抑菌作用,且效果强于革兰氏阴性菌,对真菌的抑菌作用最弱;菱角梗、菱角壳乙酸乙酯萃取物在温度20~100℃、pH5~9和紫外光处理10~40 min时,均表现出良好的抑菌稳定性。经鉴定,菱角梗乙酸乙酯萃取物中含量最高的是没食子酸和1,2,6-三-O-没食子酰-β-D-葡萄糖,含量为45.87%,菱角壳乙酸乙酯萃取物中含量最高的是1,2,3,6-四-O-没食子酰-β-D-葡萄糖、槲皮素-3-O-半乳糖苷和1,2,6-三-O-没食子酰-β-D-葡萄糖,含量为57.84%。本文为菱角梗、菱角壳提取物作为抑菌剂的实际应用提供了参考。
  • [1] Takano A,Kadono Y. Allozyme variations and classification of Trapa(Trapaceae)in Japan[J]. Aquatic Botany,2005,83(2):0-118.
    [2] 伍茶花.菱角壳化学成分及其活性研究[D].长沙:湖南中医药大学,2012.
    [3] Tulyathan V,Boondee K,Mahawanich T. Characteristics of starch from water chestnut(Trapa bispinosa ROXB.)[J]. Journal of Food Biochemistry,2005,29(4):337-348.
    [4] Wang C C R,Ciou J Y,Chiang P Y. Effect of micronization on functional properties of the water caltrop(Trapa taiwanensis Nakai)pericarp[J]. Food Chemistry,2009,113(4):970-974.
    [5] Ciou J Y,Lin H H,Chiang P Y,et al. The role of polyphenol oxidase and peroxidase in the browning of water caltrop pericarp during heat treatment[J]. Food Chemistry,2011,127(2):523-527.
    [6] Zhao W J,Niu F L,Li J,et al. Components of volatile oil from water-caltrop and their anti-tumor effect in vitro[J]. 高等学校化学研究:英文版,2009,25(1):56-59.
    [7] Zhu F. Chemical composition,health effects,and uses of water caltrop[J]. Trends in Food Science & Technology,2016,49:136-145.
    [8] 董向东,孟秀梅,王晶晶,等.菱角多糖提取及抗氧化能力研究[J].农产品加工,2017,1(2):14-16

    ,19.
    [9] Yu H N,Shen S R. Phenolic composition,antioxidant,antimicrobial and antiproliferative activities of water caltrop pericarps extract[J]. LWT-Food Science and Technology,2015,61(1):238-243.
    [10] Balasundram N,Sundram K,Samman S. Phenolic compounds in plants and agri-industrial by-products:Antioxidant activity,occurrence,and potential uses[J]. Food Chemistry,2006,99(1):191-203.
    [11] 刘冬敏,王建辉,刘永乐.菱角壳中黄酮、总酚和皂苷的提取及其抗氧化性研究[J].食品安全质量检测学报,2016,7(9):3668-3672.
    [12] 卢梦,周禹君,李丽,等.菱角壳纤维咀嚼片的研制[J].农产品加工,2018,461(15):25-26.
    [13] Yasuda M,Yasutake K,Hino M,et al. Inhibitory effects of polyphenols from water chestnut(Trapa japonica)husk on glycolytic enzymes and postprandial blood glucose elevation in mice[J]. Food Chemistry,2014,165(15):42-49.
    [14] 左袁袁,吕寒,吴月娴,等.不同产地菱角壳(欧菱果壳)中总多酚含量及抗氧化活性和对α-葡萄糖苷酶活性抑制作用的比较[J].植物资源与环境学报,2018,27(3):114-116.
    [15] Kim Y S,Hwang J W,Han Y K,et al. Antioxidant activity and protective effects of Trapa japonica pericarp extracts against tert-butylhydroperoxide-induced oxidative damage in Chang cells[J]. Food and Chemical Toxicology,2014,64:49-56.
    [16] Wang S H,Kao M Y,Wu S C,et al. Oral administration of Trapa taiwanensis Nakai fruit skin extracts conferring hepatoprotection from CCl4-caused injury[J]. Journal of Agricultural and Food Chemistry,2011,59(8):3686-3692.
    [17] Lin Q S,Shen J H,Ning Y,et al. Inhibitory effects of water caltrop pericarps on the growth of human gastric cancer cells in vitro[J]. Current Pharmaceutical Design,2013,19(42):7473-7478.
    [18] Huang H C,Chao C L,Liaw C C,et al. Hypoglycemic constituents isolated from Trapa natans L. pericarps[J]. Journal of Agricultural and Food Chemistry,2016,64(19):3794-3803.
    [19] 董晶莱,高广春,黄嬛,等.菱角壳的化学成分和药理活性研究进展[J].嘉兴学院学报,2014,26(6):68-71.
    [20] Raza A,Li F,Xu X,et al. Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology[J]. International Journal of Biological Macromolecules,2016,94(Pt A):335-344.
    [21] 申凯,张辉,严碧云,等.菱茎多酚的提取工艺及其抗氧化、抑菌活性研究[J].食品工业科技,2018,39(11):225-231.
    [22] 李斌,雷月,孟宪军,等.响应面试验优化超声波辅助提取蓝靛果多酚工艺及其抗氧化活性[J].食品科学,2015,515(22):57-63.
    [23] 尹秀梅,王思宏,李东浩.八宝景天乙酸乙酯提取物的抗菌活性和化学成分的研究[J].天然产物研究与开发,2018(6):930-935.
    [24] Zaidan M R S,Rain A N,Badrul A R,et al. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method[J]. Tropical Biomedicine,2006,22(2):165-170.
    [25] Bahadori M B,Valizadeh H,Asghari B,et al. Chemical composition and antimicrobial,cytotoxicity,antioxidant and enzyme inhibitory activities of Salvia spinosa L[J]. Journal of Functional Foods,2015,18(Part A):727-736.
    [26] Carovic-Stanko K,Orlic S,Politeo O,et al. Composition and antibacterial activities of essential oils of seven Ocimum taxa[J]. Food Chemistry,2010,119(1):196-201.
    [27] 卢凤来,魏欢,王磊,等.大孔树脂HP-20和HP-SS分离纯化山银花中灰毡毛忍冬皂苷乙和川续断皂苷乙(英文)[J].Agricultural Science & Technology,2016,17(4):765-768.
    [28] 贾瑞博,李燕,陈竟豪,等.灰树花多酚HPLC分析方法优化及组分的液质定性[J].食品科技,2016(9):278-282.
    [29] 邓俊琳,朱永清,夏陈,等.绞股蓝中6种多酚化合物的HPLC检测及其醇提液的抗氧化活性[J].食品科学,2019,40(14):265-269.
    [30] Lu J,Wang Z,Ren M,et al. Antibacterial effect of gallic acid against Aeromonas hydrophila and Aeromonas sobria through damaging membrane integrity[J]. Current Pharmaceutical Biotechnology,2016,17(13):1153-1158.
  • 加载中
计量
  • 文章访问数:  4
  • HTML全文浏览量:  0
  • PDF下载量:  1
  • 被引次数: 0
出版历程
  • 收稿日期:  2020-04-08

目录

    /

    返回文章
    返回

    重要通知

    从2020年12月23日起,若所投稿件的参考文献为中文,则需补充此中文参考文献的英文格式,特此通知。