响应面法优化微波提取枇杷花槲皮素工艺及其对酒精分解关键酶活性的影响

徐伟 马智宇 李佳美 陈华 范洪臣 张光

徐伟, 马智宇, 李佳美, 陈华, 范洪臣, 张光. 响应面法优化微波提取枇杷花槲皮素工艺及其对酒精分解关键酶活性的影响[J]. 食品工业科技, 2021, 42(3): 127-132,139. doi: 10.13386/j.issn1002-0306.2020040244
引用本文: 徐伟, 马智宇, 李佳美, 陈华, 范洪臣, 张光. 响应面法优化微波提取枇杷花槲皮素工艺及其对酒精分解关键酶活性的影响[J]. 食品工业科技, 2021, 42(3): 127-132,139. doi: 10.13386/j.issn1002-0306.2020040244
XU Wei, MA Zhiyu, LI Jiamei, CHEN Hua, FAN Hongchen, ZHANG Guang. Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme[J]. Science and Technology of Food Industry, 2021, 42(3): 127-132,139. doi: 10.13386/j.issn1002-0306.2020040244
Citation: XU Wei, MA Zhiyu, LI Jiamei, CHEN Hua, FAN Hongchen, ZHANG Guang. Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme[J]. Science and Technology of Food Industry, 2021, 42(3): 127-132,139. doi: 10.13386/j.issn1002-0306.2020040244

响应面法优化微波提取枇杷花槲皮素工艺及其对酒精分解关键酶活性的影响

doi: 10.13386/j.issn1002-0306.2020040244
基金项目: 

企业横向课题(201703210300804)。

详细信息
    作者简介:

    徐伟(1963-),女,博士,教授,研究方向:微生物发酵工程,E-mail:xuw@hrbcu.edu.cn。

  • 中图分类号: TS202.1

Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme

  • 摘要: 以阴干的枇杷花及花蕾为原料,采用60%乙醇为溶剂,微波辅助提取其中的槲皮素,研究料液比、微波时间、微波功率对枇杷花槲皮素提取量的影响,采用响应面法Box-Behnken组合设计三因素三水平优化槲皮素提取条件。结果表明,最佳提取条件是料液比1∶15 g/mL、微波提取时间25 min、微波提取功率600 W;在此条件下提取量为8.26 mg/g。将枇杷花槲皮素使用HP-20型树脂柱层析纯化后纯度为64.87%。该纯化品红外光谱扫描分析结果表明:在1663、1611、1382 cm-1处有槲皮素特征峰。探讨枇杷花槲皮素对酒精分解过程中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、过氧化氢酶(CAT)活性的影响,结果表明酶活力均有一定的提高,激活率为24.83%、29.18%、22.36%。枇杷花槲皮素对酒精分解过程中关键酶均有一定的激活作用,为开发天然产物解酒护肝辅助食品提供一定理论基础。
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  • 收稿日期:  2020-04-21

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