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中国精品科技期刊2020
谭莉,孟繁磊,范宏,等. 超高效液相色谱-串联质谱法同时测定玉米中9种真菌毒素[J]. 食品工业科技,2021,42(7):240−245. doi: 10.13386/j.issn1002-0306.2020050057.
引用本文: 谭莉,孟繁磊,范宏,等. 超高效液相色谱-串联质谱法同时测定玉米中9种真菌毒素[J]. 食品工业科技,2021,42(7):240−245. doi: 10.13386/j.issn1002-0306.2020050057.
TAN Li, MENG Fanlei, FAN Hong, et al. Determination of 9 Kinds of Mycotoxins in Maize by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(7): 240−245. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050057.
Citation: TAN Li, MENG Fanlei, FAN Hong, et al. Determination of 9 Kinds of Mycotoxins in Maize by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(7): 240−245. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020050057.

超高效液相色谱-串联质谱法同时测定玉米中9种真菌毒素

Determination of 9 Kinds of Mycotoxins in Maize by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

  • 摘要: 为高效检测玉米中的生物毒素,采用80%乙腈−0.1%甲酸水溶液提取、QuEChERS法净化、甲醇−0.1%甲酸+5 mmol/L甲酸铵水溶液经C18柱梯度洗脱分离、电喷雾正负离子同时扫描并多反应监测模式采集数据的综合方法,对玉米中黄曲霉毒素B1、B2、伏马毒素B1、B2、B3、呕吐毒素、3-乙酰脱氧雪腐镰刀菌烯醇、15-乙酰脱氧雪腐镰刀菌烯醇及玉米赤霉烯酮9种真菌毒素进行同时测定。结果表明,9种真菌毒素在0.25~250 μg/L浓度范围内线性关系良好,相关系数均在0.9999以上,检出限在0.079~1.8 μg/kg之间,定量限在0.26~6.0 μg/kg之间,回收率在80.2%~113.8%之间,相对标准偏差≤7.7%。用该方法同时检测吉林省240份玉米样品中9种真菌毒素,其中未检测出黄曲霉毒素B1、B2,但其它7种毒素均有检出,其中ZEN检出率最小,为14.6%,FB2检出率最大,达到98.3%。本方法操作简单、灵敏度高、重现性好,结果准确,适用于玉米中多组分真菌毒素同时检测。

     

    Abstract: In order to effectively detect the biological toxins in maize, 80% acetonitrile -0.1% formic acid aqueous solution extraction, QuEChERS purification, methanol −0.1% formic acid +5 mmol/L ammonium formate aqueous solution using C18 column and gradient elution separation, electrospray positive and negative ions simultaneous scanning, and multiple reaction monitoring mode synthesis method of gathering data were applied to simultaneously determine nine mycotoxins including aflatoxin B1, aflatoxin B2, fumonisin B1, fumonisin B2, fumonisin B3, vomitoxin, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol and zearalenone. Results showed that, the linear relationship of 9 kinds of mycotoxins were good in the concentration range of 0.25~250 μg/L, the correlation coefficient was above 0.9999, the detection limit was 0.079~1.8 μg/kg, the quantitative limit was 0.26~6.0 μg/kg, the recovery rate was 80.2%~113.8%, the relative standard deviation ≤ 7.7%. Nine mycotoxins were detected in 240 maize samples in Jilin province by this method, in which the aflatoxin B1, B2 were not detected, but the other seven toxins were detected. The detection rate of zearalenone was the lowest (14.6%) and FB2 was the highest (98.3%). This method is simple, sensitive, reproducible and accurate. It is suitable for the simultaneous determination of mycotoxins in maize.

     

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