Extraction Process Optimization and Antioxidant Activity of Polyphenols from Bangia fusco-purpurea
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摘要: 为研究红毛藻多酚提取工艺及其抗氧化活性，采用溶剂浸提法提取多酚，选取提取时间、提取温度、乙醇体积分数、料液比为单因素，进一步通过正交试验优化确定最佳提取工艺。以DPPH和ABTS自由基清除率为指标，评价红毛藻多酚的抗氧化活性。结果显示，红毛藻多酚的最佳提取工艺为：浸提温度70 ℃，浸提时间70 min，乙醇体积分数60%，料液比1:10 g/mL，此条件下多酚提取量为（9.67 ± 0.14）mg/g。抗氧化活性评价显示，红毛藻多酚对DPPH和ABTS自由基有一定的清除能力，其IC50值分别为0.313、0.445 mg/mL。研究结果表明正交优化提取红毛藻多酚的工艺简单可行，此方法提取多酚具有较强的抗氧化活性。红毛藻多酚具有开发为天然抗氧化剂的潜力，有较好的应用前景。Abstract: In order to study the extraction process and antioxidative activity of polyphenols from Bangia fusco-purpurea, solvent extraction was used to extract polyphenols. The polyphenol content in the extract was used as an index. The extraction time, extraction temperature, ethanol volume fraction, and liquid-liquid ratio were single factors. Further, the optimal extraction process was determined by orthogonal experiment optimization. The clearance rate of DPPH and ABTS free radical were used as indicators to evaluate the antioxidative activity of B. fusco-purpurea polyphenols. The results showed that the optimum extraction conditions were as follows: Extraction temperature 70 ℃, extraction time 70 min, volume fraction of ethanol 60%, ratio of material to liquid 1:10 g/mL.Under these conditions, the extraction amount of B. fusco-purpurea polyphenols was (9.67 ± 0.14) mg/g. Antioxidant activity evaluation showed that B. fusco-purpurea polyphenols had a certain scavenging ability to DPPH• and ABTS+•, with IC50 values of 0.313, 0.445 mg/mL. The results showed that the orthogonal optimization process for extracting B. fusco-purpurea polyphenol was simple and feasible, the polyphenols had certain antioxidant capacity. B. fusco-purpurea polyphenol has the potential to be developed as a natural antioxidant and has a good application prospect.
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Key words:
- Bangia fusco-purpurea /
- polyphenol /
- extract technology /
- antioxidant activity
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图 1 提取时间对红毛藻多酚提取量的影响
Figure 1. Effect of extraction time fraction on polyphenol extraction of Bangia fusco-purpurea
图 2 提取温度对红毛藻多酚提取的影响
Figure 2. Effect of extraction temperature on polyphenol extraction of Bangia fusco-purpurea
图 3 料液比对红毛藻多酚提取的影响
Figure 3. Effect of material to liquid ratio on polyphenol extraction of Bangia fusco-purpurea
图 4 乙醇体积分数对红毛藻多酚提取的影响
Figure 4. Effect of ethanol volume fraction on polyphenol extraction of Bangia fusco-purpurea
图 5 红毛藻多酚对DPPH·的清除效果
Figure 5. DPPH·radical−scavenging activities of polyphenols of Bangia fusco-purpurea
图 6 红毛藻多酚对ABTS+·的清除效果
Figure 6. ABTS+· radical−scavenging capacity of polyphenols of Bangia fusco-purpurea
表 1 正交试验因素水平表
Table 1. Factors and levels of orthogonal test
水平 A提取时间（min） B提取温度（℃） C料液比（g/mL） D乙醇体积分数（%） 1 50 65 1:10 55 2 60 70 1:20 60 3 70 75 1:30 65 
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表 2 正交试验结果
Table 2. The results of orthogonal test
试验号 A B C D 多酚提取量（mg/g） 1 1 1 1 1 8.33 ± 0.13 2 1 2 2 2 8.61 ± 0.17 3 1 3 3 3 6.05 ± 0.15 4 2 1 2 3 7.6 ± 0.14 5 2 2 3 1 6.19 ± 0.16 6 2 3 1 2 8.70 ± 0.17 7 3 1 3 2 6.74 ± 0.13 8 3 2 1 3 9.23 ± 0.14 9 3 3 2 1 8.95 ± 0.11 k1 7.67 7.56 8.76 7.83 k2 7.50 8.01 8.39 8.02 k3 8.31 7.90 6.33 7.63 R 0.81 0.45 2.43 0.39
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表 3 方差分析结果
Table 3. The variance analysis results
因素 偏差平方和 自由度 均方 F值 显著性 A 3.264 2 1.632 77.913 ** B 1.006 2 0.503 24.023 ** C 30.711 2 15.355 733.015 ** D 0.684 2 0.342 16.337 ** 误差 0.377 18 0.021 注：**表示极显著，P<0.01；*表示显著，P<0.05。
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[1] 田其然. 经济红藻红毛菜的生物学特性研究[D]. 汕头: 汕头大学, 2007. [2] 黄文, 黄建明, 董飞强, 等. 红毛菜的营养成分特征和价值[J]. 海洋水产研究,1998(2):57−61. [3] 吴靖娜, 陈晓婷, 路海霞, 等. 红毛藻营养成分分析与评价[J]. 食品与生物技术学报,2019,38(6):131−136. doi: 10.3969/j.issn.1673-1689.2019.06.018 [4] Cofrades S, Lopez-Lopez I, Bravo L, et al. Nutritional and antioxidant properties of different brown and red Spanish edible seaweeds[J]. Food Science & Technology International,2010,16(5):361−370. [5] 纪乃茹, 李月, 姜泽东, 等. 红毛藻研究进展[J]. 食品工业,2020,41(2):238−241. [6] 付晓苹. 红毛藻及坛紫菜藻红蛋白的分离纯化与相关性质的研究[D]. 厦门: 集美大学, 2007. [7] Ank G, Da Gama B A P, Pereira R C. Polyphenols from Stypopodium zonale (Phaeophyceae): Intrapopulational variation, induction by simulated herbivory, and epibiosis effects[J]. Aquatic Botany,2013,111:125−129. doi: 10.1016/j.aquabot.2013.06.007 [8] Emer S, Nissreen A G. Antibacterial derivatives of marine algae: An overview of pharmacological mechanisms and applications[J]. Marine Drugs,2016,14(4):91. [9] Kim H J, Dasagrandhi C, Kim S H, et al. In vitro antibacterial activity of phlorotannins from edible brown algae, eisenia bicyclis against streptomycin-resistant Listeria monocytogenes[J]. Indian Journal of Microbiology,2018,58(1):105−108. doi: 10.1007/s12088-017-0693-x [10] Van T T T, Hieu V M N, Vi T N H, et al. Antioxidant activities and total phenolic content of macroalgae from central coast of Vietnam[J]. Asian Journal of Chemistry,2013,25(12):6639−6642. doi: 10.14233/ajchem.2013.14395 [11] 李亚娴. 羊栖菜多酚粗品的纯化及其抗肿瘤活性研究[D]. 大连: 大连海洋大学, 2016. [12] Pantidos N, Boath A, Lund V, et al. Phenolic-rich extracts from the edible seaweed, Ascophyllum nodosum, inhibit α-amylase and α-glucosidase: Potential anti-hyperglycemic effects[J]. Journal of Functional Foods,2014,10:201−209. doi: 10.1016/j.jff.2014.06.018 [13] Lee S H, Jeon Y J. Anti-diabetic effects of brown algae derived phlorotannins, marine polyphenols through diverse mechanisms[J]. Fitoterapia,2013,86:129−136. doi: 10.1016/j.fitote.2013.02.013 [14] Yuan Y, Zhang J, FAN J J, et al. Microwave assisted extraction of phenolic compounds from four economic brown macroalgae species and evaluation of their antioxidant activities and inhibitory effects on α-amylase, α-glucosidase, pancreatic lipase and tyrosinase[J]. Food Research International,2018,113:288−297. doi: 10.1016/j.foodres.2018.07.021 [15] Montero L, Sánchez-Camargo A D, Ibáñez, E, et al. Phenolic compounds from edible algae: Bioactivity and health benefits[J]. Current Medicinal Chemistry,2019,25(37):4808−4826. doi: 10.2174/0929867324666170523120101 [16] 彭雍博, 罗宣, 汪秋宽, 等. 海藻多酚功能性作用机制及其应用研究[J]. 大连海洋大学学报,2017,32(4):484−492. [17] 赵国玲, 刘承初. 不同种类海藻多酚含量和还原力的对比研究[C]//中国水产学会, 中国水产学会学术年会论文摘要集.海口: 中国水产学会, 2009: 161. [18] 孙洲悦, 陈思远, 陈永雄, 等. 辣木叶多酚提取条件的优化及其抑菌性能研究[J]. 食品工业,2018,39(5):70−74. [19] 崔錾, 吕芳, 王天一, 等. 天浆壳多酚的提取工艺优化及其抗氧化活性评价[J]. 食品工业科技,2017,38(22):167−172. [20] 马慧, 茹鑫, 王津, 等. 4种茶叶水提物及茶多酚的体外抗氧化性能研究[J]. 食品研究与开发,2019,40(8):65−70. doi: 10.3969/j.issn.1005-6521.2019.08.011 [21] 胡会刚, 赵巧丽. 菠萝皮渣多酚的提取分离及其抗氧化活性评价[J]. 食品科技,2020,45(1):286−293. [22] Yang L, Jiang J G, Li W F, et al. Optimum extraction process of polyphenols from the bark of Phyllanthus emblica L. based on the response surface methodology[J]. Journal of Separation Science,2009,32(9):1437−1444. doi: 10.1002/jssc.200800744 [23] 洪璇, 陈仲巍, 李鹤宾, 等. 鸡桑叶多酚提取工艺优化及其抗氧化性能[J]. 食品工业科技,2018,39(13):73−77. [24] 付钦宝. 荷叶多酚的提取、抗氧化活性及其应用研究[D]. 芜湖: 安徽工程大学, 2017. [25] Jovanović A A, Đorđević V B, Zdunić G M, et al. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques[J]. Separation and Purification Technology,2017,179:369−380. doi: 10.1016/j.seppur.2017.01.055 [26] 史建鑫. 昆布多酚提取、抗氧化与抑菌活性的研究[D]. 南昌: 南昌大学, 2017. [27] 陈洪彬, 杨敏, 宋露露, 等. 龙须菜多酚提取工艺优化及其体外抗氧化活性[J]. 食品与机械,2017,33(4):139−143. [28] 王敏, 田珍燕, 王蔚新. 板栗壳多酚的提取及其抗氧化性能的研究[J]. 食品研究与开发,2018,39(22):66−72. doi: 10.3969/j.issn.1005-6521.2018.22.012 [29] Dang T T, Quan V V, Schreider M J, et al. The effects of drying on physico-chemical properties and antioxidant capacity of the brown alga (Hormosira banksii (Turner) Decaisne)[J]. Journal of Food Processing and Preservation,2017,41(4):1−11. [30] 段宙位, 窦志浩, 何艾, 等. 青金桔皮中多酚的提取及其抗氧化性研究[J]. 食品工业科技,2015,36(10):244−248. [31] 谢惠, 韩娅婷, 邵佩兰, 等. 红枣可溶性膳食纤维的抗氧化活性研究[J]. 食品工业科技,2017,38(22):37−41. [32] 许亚如. 褐藻多酚的抗氧化活性研究[D]. 宁波: 宁波大学, 2014. -
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