适配体生物传感器检测食品中链霉素的研究进展

彭海帅; 王毕妮; 惠媛媛; 张富新; 刘玉芳; 赵爱青; 任荣; 贾蓉

doi:10.13386/j.issn1002-0306.2020070131

适配体生物传感器检测食品中链霉素的研究进展

doi: 10.13386/j.issn1002-0306.2020070131

陕西师范大学食品工程与营养科学学院，陕西西安 710119

基金项目: 陕西省重点研发计划重点产业创新链（群）项目（2019ZDLNY06-06）；中央高校基本科研业务项目（GK202003084）

详细信息

作者简介:
彭海帅（1996−），女，硕士研究生，研究方向：食品安全快速检测，E-mail：phs13607093130@163.com

通讯作者:
王毕妮（1980−），女，博士，教授，研究方向：食品安全快速检测，E-mail：biniwang@snnu.edu.cn

中图分类号: TS207.3
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- 被引次数: 0
出版历程
- 收稿日期: 2020-07-13
- 网络出版日期: 2021-06-04
- 刊出日期: 2021-07-07

Research Progress of Aptamer Biosensor Detection of Streptomycin in Food

College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710119, China

摘要

摘要: 链霉素（Streptomycin, STR）是一种氨基糖苷类抗生素，广泛用于水产、畜牧业中治疗细菌性疾病。人类食用的动物性食品中若残留过量的STR会严重威胁人体健康。生物传感器作为快速检测技术可以实现食品中STR快速、准确检测，基于适配体的生物传感器因表现出许多独特优势而被广泛用于食品安全检测领域。本文综述了近五年基于适配体的光学、电化学生物传感器在检测动物性食品中STR残留的应用进展，并对这些检测技术进行对比和总结，以期为今后发展更为有效、简便、灵敏的STR生物传感器提供一定的参考。
- 链霉素 /
- 适配体 /
- 生物传感器 /
- 快速检测 /
- 食品安全
Abstract: Streptomycin (STR) is an aminoglycoside antibiotic widely used in aquaculture and animal husbandry to treat bacterial diseases. Excessive STR in animal foods that humans eat can seriously threaten human health. As a rapid detection technology, biosensors can achieve rapid and accurate detection of STR in foods. Biosensors based on aptamers are widely used in the field of food safety detection because of their unique advantages. In this paper, the application progress of optical and electrochemical biosensors based on aptamers in the detection of STR residue in animal food in the past five years is reviewed, and these detection techniques are compared and summarized, in order to provide some reference for the development of more effective, simple and sensitive STR biosensors in the future.
- streptomycin /
- aptamer /
- biosensor /
- rapid detection /
- food safety

HTML全文

表 1 链霉素光学适配体传感器

Table 1. Streptomycin optical aptamer sensor

检测原理	检测方法	检测范围	检出限	参考文献
化学发光法	基于G-四链体DNA酶修饰的三维石墨烯化学发光适配体传感器	1.4×10⁻¹²~2.8×10⁻⁹ mol/L	9.2×10⁻¹⁴ mol/L	[25]
荧光法	基于基于ExoIII、SYBR Gold和适配体互补链的荧光传感器	−	54.5 nmol/L	[29]
荧光法	基于AuNPs和dsDNA荧光猝灭适配体传感器	−	47.6 nmol/L	[30]
比色法	以多孔SiO₂微珠-酶联聚合物为比色探针的比色传感器	0.003~20 ng/mL	1 pg/mL	[36]
	基于智能手机的比色法适配体传感器	0.1~100 μmol/L	94 nmol/L	[37]
注：−文中未说明。

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表 2 链霉素电化学适配体传感器

Table 2. Streptomycin electrochemical aptamer sensor

检测方法	检测范围	检出限	孵育时间（h）	反应时间（min）	参考文献
基于AuNPs和GQD-SH的电化学适配体传感器	0.1~700 pg/mL	0.033 pg/mL	2	−	[47]
基于CNFs和OMC-AuNPs的电化学适配体传感器	0.1~1000 nmol/L	45.0 pmol/L	1	40	[51]
基于MWCNTGr和CNFs-AuNPs纳米复合材料电化学适配体传感器	0.1~100 nmol/L	36.45 pmol/L	1	40	[53]
基于PCNR/GR-Fe₃O₄-AuNPs纳米复合材料电化学适配体传感器	0.05~200 ng/mL	0.028 ng/mL	2	−	[55]
基于Au @ MWCNTs-Fe₃O₄和NP-PtTi的电化学适配体传感器	0.05~100 ng/mL	7.8 pg/mL	−	120	[56]
基于MSFs、金电极和AgNPs的电化学适配体传感器	1 fg/mL~6.2 ng/mL	0.33 fg/mL	2	−	[49]
基于Pt-Sn@TiO₂复合材料和核酸外切酶的电化学适配体传感器	0.05~1500 nmol/L	（0.020±0.0045）nmol/L	1.5	−	[48]
基于TiO₂/BiOI/BiOBr光电化学适配体传感器	0.05~150 nmol/L	0.04 nmol/L	2	−	[63]
基于Mo-BiVO₄和石墨烯纳米复合材料光电化学适配体传感器	0.1~100 nmol/L	0.0481 nmol/L	−	−	[60]
基于CdTe量子点和SWCNHs光电化学适配体传感器	0.1~50 nmol/L	0.033 nmol/L	0.67	−	[64]
基于双重信号检测的光电化学适配体传感器	0.03~100 μmol/L	10 nmol/L	14	60	[66]
基于LaFeO₃@g-C₃N₄p-n异质结构光电化学适配体传感器	0.01~10000 nmol/L	0.0033 nmol/L	−	−	[68]
基于CdTe QDs与WO₃纳米片光电化学适配体传感器	1.5~728.5 μg/kg	0.5 μg/kg	0.5	−	[59]
注：−文中未说明。

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