贝类致敏原抗原表位及交叉反应的研究进展

桓霏; 云肖; 李梦思; 韩天娇; 刘萌; 杨阳; 曹敏杰; 刘光明

doi:10.13386/j.issn1002-0306.2020080238

贝类致敏原抗原表位及交叉反应的研究进展

doi: 10.13386/j.issn1002-0306.2020080238

桓霏^{1, 2,},
云肖^{1, 2},
李梦思^{1, 2},
韩天娇^{1, 2},
刘萌^{1, 2},
杨阳^{1, 2},
曹敏杰^{1, 2},
刘光明^{1, 2, ,}

1.
集美大学食品与生物工程学院，福建厦门 361021
2.
水产品深加工技术国家地方联合工程研究中心，厦门市海洋功能食品重点实验室, 福建厦门 361021

基金项目: 国家重点研发计划项目（2019YFD0901703）；国家自然科学基金项目（318717203，32072336，32001695，31901811）；福建省科技计划项目（2018N5009，2018R0071）；海洋科研公共部门专项基金项目（DY135-B2-07，201505026-03）

详细信息

作者简介:
桓霏（1997−），女，硕士研究生，研究方向：水产品过敏，E-mail：1225252857@qq.com

通讯作者:
刘光明（1972−），男，博士，教授，研究方向：食品加工与安全，E-mail：gmliu@jmu.edu.cn

中图分类号: TS254.1
计量
- 文章访问数: 88
- HTML全文浏览量: 53
- PDF下载量: 10
- 被引次数: 0
出版历程
- 收稿日期: 2020-08-24
- 网络出版日期: 2021-06-08
- 刊出日期: 2021-07-07

Research Progress on Epitopes and Cross-reactivity of Shellfish Allergens

HUAN Fei^{1, 2
,},
YUN Xiao^{1, 2},
LI Mengsi^{1, 2},
HAN Tianjiao^{1, 2},
LIU Meng^{1, 2},
YANG Yang^{1, 2},
CAO Minjie^{1, 2},
LIU Guangming^{1, 2
, ,}

1.
College of Food and Bioscience Engineering, Jimei University, Xiamen 361021, China
2.
Key Laboratory of Marine Functional Food in Xiamen, National Joint Engineering Research Center for Deep Processing of Aquatic Products, Xiamen 361021, China

摘要

摘要: 贝类（Shellfish）包括甲壳类和软体类，具有很高的营养价值和经济价值。我国作为贝类生产及消费大国，由食用贝类引发的食物过敏问题日益增多。已报道的贝类食品致敏原有原肌球蛋白、精氨酸激酶、肌质钙结合蛋白、肌球蛋白轻链等。抗原表位是食品致敏原发挥作用的分子基础，明确不同致敏原的抗原表位，是深入分析其致敏性及交叉反应性的关键，也是定向消减致敏原致敏性的重要前提。本文介绍了贝类食品致敏原种类、抗原表位、交叉反应性和加工消减致敏原致敏性等方面的研究进展。
- 贝类 /
- 食物过敏 /
- 致敏原 /
- 抗原表位 /
- 交叉反应 /
- 加工消减
Abstract: Shellfish, including crustaceans and molluscs, have high nutritional value and economic value. As a big producer and consumer of shellfish, food allergies caused by eating shellfish are increasing in China. The identified shellfish allergens include tropomyosin, arginine kinase, sarcoplasmic-calcium-binding protein, myosin light chain, etc. Epitope is the molecular basis for the action of allergens. To identify antigenic epitopes of different allergens is the key to further analysis of their sensitization and cross-reactivity, and is also an important prerequisite for directional reduction of sensitization. In this paper, the research progress of shellfish allergen species, antigenic epitopes, cross-reactivity and processing reduction allergen sensitization are introduced.
- shellfish /
- food allergy /
- allergen /
- epitope /
- cross-reactivity /
- allergenicity reducing

HTML全文

图 1 贝类TM线性表位定位^[12,24-29]

Figure 1. Linear epitopes location of shellfish TM^[12,24-29]

注：带*表示预测的抗原表位；图2~图4同。

下载: 全尺寸图片幻灯片

图 2 贝类AK线性表位定位^[35-39]

Figure 2. Linear epitopes location of shellfish AK^[35-39]

下载: 全尺寸图片幻灯片

图 3 贝类SCP线性表位定位^[37,41]

Figure 3. Linear epitopes location of shellfish SCP

下载: 全尺寸图片幻灯片

图 4 贝类MLC线性表位定位^[37,47-48]

Figure 4. Linear epitopes location of shellfish MLC

下载: 全尺寸图片幻灯片

表 1 贝类四种常见致敏原的构象表位

Table 1. Conformational epitopes of four commonly shellfish allergens

致敏原	构象表位
TM^[27]	K₅A₃K₆Q₉D₂I₄A₁₀I₄
	L₈₈I₉₂A₈₆L₉₄R₉₀V₈₅
	K₁₂₈R₁₃₃S₁₃₄L₁₃₅V₁₂₉N₁₃₂R₁₂₅S₁₃₆S₁₃₄
	L₁₆₉V₁₇₂L₁₇₆M₁₇₁V₁₆₅A₁₇₀L₁₇₆A₁₇₄L₁₆₉
	N₂₃₀A₂₂₉A₂₃₄K₂₂₆T₂₂₇K₂₃₁A₂₃₅A₂₃₇
	I₂₇₀K₂₆₈K₂₆₄Y₂₆₇L₂₇₄E₂₆₃I₂₇₀T₂₇₁
	T₂₇₇Q₂₇₆T₂₇₇S₂₇₉F₂₇₈L₂₇₄S₂₈₂L₂₈₁S₂₇₉
AK^{[38, 51]}	L₁₇A₁₉A₂₀T₂₁D₂₂C₂₃K₂₄K₂₈V₅₈L₆₁D₆₂
	G₁₀₅D₁₀₆V₁₀₇N₁₀₈Q₁₀₉F₁₁₀V₂₄₇F₃₃₆
	L₄₆G₄₇A₄₈N₁₃₇P₁₃₈C₁₃₉L₁₄₀T₁₄₁Q₁₄₄N₁₉₉
	S₁₅₃S₁₅₄T₁₅₅S₁₅₇N₁₅₈L₁₅₉L₁₆₃T₁₆₆R₂₅₆
	S₁₅₇G₁₆₅T₁₆₆Y₁₆₇Y₁₆₈P₁₆₉V₁₇₇L₁₈₁I₁₈₂H₂₁₂D₂₁₄
	K₁₄₆P₁₆₉L₁₇₀T₁₇₁G₁₇₂M₁₇₃L₁₈₁L₁₈₇P₂₀₅T₂₀₆G₂₀₇
	T₁₇₁Q₁₉₆N₁₉₉R₂₀₂Y₂₀₃W₂₀₄P₂₀₅T₂₀₆
	I₁₂₁H₁₈₅K₁₈₉G₃₁₀T₃₁₁R₃₁₂T₃₁₆A₃₁₈E₃₁₉G₃₂₀V₃₂₂
SCP^[51]	A₃₄L₃₅N₃₇T₃₈L₃₉I₄₀G₄₂R₄₃Y₅₁
	L₃₅N₃₇T₃₈L₃₉I₄₀E₄₁G₄₂R₄₃N₅₄T₁₁₂
	R₄₃G₄₄E₄₅F₄₆S₄₈A₅₀Y₅₁A₅₂
	N₃₇A₅₀A₅₂N₅₃N₅₄Q₅₅K₅₆I₅₇
MLC^[48]	N₄₁N₄₃P₄₄T₄₅L₄₆A₄₇I₄₈I₄₉V₇₅G₈₂
	Y₈₄S₈₃Y₈₄E₈₅F₈₇M₈₈L₁₄₆K₁₄₇K₁₄₈
	F₈₇G₈₂S₈₃Y₈₄E₈₅D₈₆F₈₇V₉₀L₉₁L₁₄₆
	F₈₇A₈₁G₈₂E₈₅F₈₇M₈₈V₉₀L₉₁L₁₄₆
	N₉₉K₉₆S₉₇E₉₈N₉₉G₁₀₀T₁₀₁Y₁₀₄F₁₃₉

下载: 导出CSV

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