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中国精品科技期刊2020

基于GC-IMS对勐海县晒青毛茶的挥发性组分分析

刘学艳 王娟 彭云 吕才有 李若愚

刘学艳,王娟,彭云,等. 基于GC-IMS对勐海县晒青毛茶的挥发性组分分析[J]. 食品工业科技,2021,42(14):233−240. doi:  10.13386/j.issn1002-0306.2020080295
引用本文: 刘学艳,王娟,彭云,等. 基于GC-IMS对勐海县晒青毛茶的挥发性组分分析[J]. 食品工业科技,2021,42(14):233−240. doi:  10.13386/j.issn1002-0306.2020080295
LIU Xueyan, WANG Juan, PENG Yun, et al. Volatile Component Analysis of Sun-dried Green Tea in Menghai County Based on GC-IMS[J]. Science and Technology of Food Industry, 2021, 42(14): 233−240. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020080295
Citation: LIU Xueyan, WANG Juan, PENG Yun, et al. Volatile Component Analysis of Sun-dried Green Tea in Menghai County Based on GC-IMS[J]. Science and Technology of Food Industry, 2021, 42(14): 233−240. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020080295

基于GC-IMS对勐海县晒青毛茶的挥发性组分分析

doi: 10.13386/j.issn1002-0306.2020080295
基金项目: 国家现代农业茶叶产业体系专项资金资助(CARS-19)
详细信息
    作者简介:

    刘学艳(1995−),女,硕士研究生,研究方向:茶叶加工与质量安全控制,Email:2414808053@qq.com

    通讯作者:

    吕才有(1964−),男,博士,教授,研究方向:茶叶加工与质量安全控制,Email:2495846526@qq.com

  • 中图分类号: S571.1

Volatile Component Analysis of Sun-dried Green Tea in Menghai County Based on GC-IMS

  • 摘要: 为探索云南省勐海县主产茶区的晒青毛茶的挥发性有机物特征,比较各乡镇茶叶之间的差异,本文运用GC-IMS技术对勐海县布朗山乡、勐宋乡、格朗和乡,每个乡镇4份样品,共计12份勐海大叶种晒青毛茶样品进行挥发性有机物检测和分析。PCA分析结果表明:12份样品可以分为两大组,布朗山乡的曼囡新寨茶(4号茶样)、勐宋乡的曼迈茶(8号茶样)、格朗和乡的南糯新路茶(10号茶样)为B组,剩余九份样品为A组。指纹图谱结果表明:B组的3份茶样的挥发性组分较相似,醛类、酯类含量均较高。A组样品中,布朗山乡的曼囡道坎茶(2号茶样)的醛类、酮类、酯类、醇类、烯类挥发性有机物组分种类和含量均较高,而格朗和乡的南糯石头一队茶(11号茶样)的酯类物质非常丰富。综上分析,勐海地区3个乡镇的晒青毛茶在挥发性有机物组分上没有明显的乡镇聚集性,每个产区的晒青毛茶样都有各自的特征性挥发组分,布朗山乡的道坎茶(2号茶样)丰富的挥发性物质为优质晒青毛茶原料的选取提供了新方向,同时勐宋乡的曼囡河边寨茶(5号茶样)、格朗和乡的南糯石头一队茶(11号茶样)特殊的挥发性组分为晒青毛茶的拼配提供了依据。此外,本实验也说明GC-IMS技术用于茶叶样品挥发性有机组分的快速定性分析具有良好的可行性。
  • 图  1  晒青毛茶样品挥发性香气化合物的指纹图谱

    Figure  1.  Fingerprint of volatile compounds of sun-dried green tea samples

    图  2  晒青毛茶样品的GC-IMS差异图谱

    Figure  2.  Differences fingerprint of volatile compounds in sun-dried green tea samples

    注:从左到右依次为1~12号样品的差异图谱

    图  3  1号晒青毛茶样品中挥发性化合物特征峰位置点

    Figure  3.  Characteristic peak location point of volatile compounds in sun-dried green tea sample

    图  4  12组样品的PCA(主成分分析)图

    Figure  4.  Analylsis of PCA in 12 tea samples

    表  1  勐海县12份晒青毛茶样品信息

    Table  1.   12 Sun-dried green tea samples’ informations of Menghai county

    编号茶样来源编号茶样来源茶样名称
    1布朗山乡曼囡村委会曼班一队上寨7勐宋乡曼金村委会曼开小组曼开
    2布朗山乡曼囡村委会道坎小组8勐宋乡曼迈村委会曼迈小组曼迈
    3布朗山乡班章村委会老曼峨小组9格朗和乡帕真村委会九二村帕真
    4布朗山乡曼囡村委会曼囡新寨10格朗和乡南糯山村委会新路村南糯新路
    5勐宋乡曼迈村委会河边寨11格朗和乡南糯山村委会石头一队南糯石头一队
    6勐宋乡曼金村委会曼金囡小组12格朗和乡南糯山村委会水河寨南糯水河寨
    下载: 导出CSV

    表  2  晒青毛茶样品的挥发性组分信息

    Table  2.   Sun-dried green tea samples’ volatile component informations

    类别编号化学物质中文名称CAS号分子式分子量保留指数保留时间(s)迁移时间
    醛类12-Undecenal2-十一烯醛C2463776C11H20O168.31396.8916.361.48365
    3Decanal癸醛C112312C10H20O156.31274.3740.1061.54521
    7(E)-2-Nonenal反式-2-壬醛C18829566C9H16O140.21187.8615.7931.41046
    8Nonanal壬醛(M)C124196C9H18O142.21107.8500.7271.47999
    9Nonanal壬醛(D)C124196C9H18O142.21107.8500.7271.94638
    15(E)-2-Octenal反式-2-辛烯醛(M)C2548870C8H14O126.21055.1424.9481.33328
    16(E)-2-Octenal反式-2-辛烯醛(D)C2548870C8H14O126.21055.4425.3271.82168
    18Benzeneacetaldehyde苯乙醛C122781C8H8O120.21039.3402.2181.25737
    22(E,E)-2,4-Heptadienal(E,E)-2,4-庚二烯醛C4313035C7H10O110.21012.2363.1971.19721
    23Octanal正辛醛(M)C124130C8H16O128.21005.1352.9681.40918
    24Octanal正辛醛(D)C124130C8H16O128.21004.8352.5891.82741
    32Benzaldehyde苯甲醛(M)C100527C7H6O106.1958.9309.4581.14985
    33Benzaldehyde苯甲醛(D)C100527C7H6O106.1958.4309.0761.47158
    34(E)-2-Heptenal(E)-2-庚烯醛(M)C18829555C7H12O112.2954.7306.0221.25527
    35(E)-2-Heptenal(E)-2-庚烯醛(D)C18829555C7H12O112.2953.8305.2591.66872
    38Heptanal庚醛(M)C111717C7H14O114.2899.9260.5821.33174
    39Heptanal庚醛(D)C111717C7H14O114.2901.0261.4231.69638
    44(E)-2-Hexenal2-己烯醛(M)C6728263C6H10O98.1843.5229.7411.18009
    45(E)-2-Hexenal2-己烯醛(D)C6728263C6H10O98.1844.1230.0211.51927
    46Furfural糠醛C98011C5H4O296.1823.4219.6481.08285
    48Hexanal正己醛C66251C6H12O100.2786.0200.9391.5643
    52(E)-2-Pentenal反式-2-戊烯醛(M)C1576870C5H8O84.1738.9182.6321.10385
    53(E)-2-Pentenal反式-2-戊烯醛(D)C1576870C5H8O84.1737.3181.9891.36053
    603-Methylbutanal异戊醛C590863C5H10O86.1608.0145.0961.40759
    56Pentanal戊醛(D)C110623C5H10O86.1680.9161.551.42128
    57Pentanal戊醛(M)C110623C5H10O86.1678.6161.0361.18513
    592-Methyl-butanal2-甲基丁醛C96173C5H10O86.1631.9150.4951.39818
    63Butanal丁醛C123728C4H8O72.1552.3132.4991.28952
    65Methylpropanal异丁醛C78842C4H8O72.1504.4121.7011.28096
    752-Methylhexanal2-正甲基-正己醛(M)C925542C7H14O114.2880.5248.3011.3204
    762-Methylhexanal2-正甲基-正己醛(D)C925542C7H14O114.2879.8247.9361.70567
    醇类2Citronellol香茅醇C106229C10H20O156.31279.6747.7691.34514
    10Linalool芳樟醇C78706C10H18O154.31103.3494.2371.22148
    13Linalool oxide氧化芳樟醇C60047178C10H18O2170.31085.6468.8491.26186
    192-Ethylhexanol2-乙基己醇C104767C8H18O130.21030.3389.3371.41921
    281-Octen-3-ol1-辛烯-3-醇(M)C3391864C8H16O128.2983.7330.0731.15807
    291-Octen-3-ol1-辛烯-3-醇(D)C3391864C8H16O128.2982.4328.9281.59753
    303-Furanmethanol3-呋喃甲醇C4412913C5H6O298.1976.4323.9651.10741
    431-Hexanol正己醇C111273C6H14O102.2866.4241.2361.32248
    491-Pentanol1-戊醇(M)C71410C5H12O88.1749.7186.7461.25387
    501-Pentanol1-戊醇(D)C71410C5H12O88.1750.1186.9331.5134
    512-Methyl-1-butanol2-甲基丁醇C137326C5H12O88.1743.3184.3181.22492
    662-Propanol异丙醇C67630C3H8O60.1485.1117.3311.19455
    68Ethanol乙醇C64175C2H6O46.1378.193.1581.04606
    72Methanol甲醇C67561CH4O32.0357.788.5560.98535
    843-Methyl-1-pentanol3-甲基-1-戊醇C589355C6H14O102.2841.5228.7621.60923
    酯类4Ethyl 2-phenylacetate苯乙酸乙酯C101973C10H12O2164.21238.8689.1011.29683
    5Methyl Salicylate水杨酸甲酯C119368C8H8O3152.11229.8676.111.20016
    6Ethyl benzoate苯甲酸乙酯C93890C9H10O2150.21208.5645.4871.26291
    11Methyl benzoate苯甲酸甲酯(M)C93583C8H8O2136.11093.3479.9151.22148
    12Methyl benzoate苯甲酸甲酯(D)C93583C8H8O2136.11092.0477.9621.6059
    25Butyl butanoate丁酸丁酯C109217C8H16O2144.21000.6346.5281.3433
    36Butyrolactoneγ-丁内酯C96480C4H6O286.1916.8274.6011.08054
    61Ethyl Acetate乙酸乙酯(M)C141786C4H8O288.1568.2136.0981.09701
    62Ethyl Acetate乙酸乙酯(D)C141786C4H8O288.1569.9136.4841.33657
    70Butyl propanoate丙酸丁酯(M)C590012C7H14O2130.2906.1265.7111.28794
    71Butyl propanoate丙酸丁酯(D)C590012C7H14O2130.2906.9266.3831.72563
    77Butyl acetate乙酸丁酯(M)C123864C6H12O2116.2800.9208.3661.23685
    78Butyl acetate乙酸丁酯(D)C123864C6H12O2116.2800.6208.2441.61864
    79Ethyl propanoate丙酸乙酯(M)C105373C5H10O2102.1696.1166.1561.15083
    80Ethyl propanoate丙酸乙酯(D)C105373C5H10O2102.1693.3165.0911.45449
    酮类14Acetophenone苯乙酮C98862C8H8O120.21060.6432.9041.18718
    276-Methyl-5-hepten-2-one甲基庚烯酮C110930C8H14O126.2991.6336.6221.17589
    311-Octen-3-one1-辛烯-3-酮C4312996C8H14O126.2980.0327.0191.27443
    402-Heptanone2-庚酮(M)C110430C7H14O114.2890.5253.2921.25997
    412-Heptanone2-庚酮(D)C110430C7H14O114.2890.5253.2921.63271
    42Cyclohexanone环己酮C108941C6H10O98.1894.9256.3761.15231
    54Acetoin3-羟基-2-丁酮(M)C513860C4H8O288.1720.5175.5621.07562
    55Acetoin3-羟基-2-丁酮(D)C513860C4H8O288.1721.5175.9471.33144
    642-Butanone2-丁酮C78933C4H8O72.1549.4131.8561.24588
    672-Propanone丙酮C67641C3H6O58.1442.9107.8121.11575
    812-Hexanone2-己酮(M)C591786C6H12O100.2775.9196.821.18689
    822-Hexanone2-己酮(D)C591786C6H12O100.2775.3196.6071.50103
    烯类17beta-Ocimeneβ-罗勒烯C13877913C10H16136.21053.0421.9171.22156
    20Limonene柠檬烯(D)C138863C10H16136.21024.0380.2451.30177
    21Limonene柠檬烯(M)C138863C10H16136.21024.8381.3811.21726
    73alpha-Pineneα-蒎烯C80568C10H16136.2930.7286.0851.21822
    杂环类262-Pentylfuran2-正戊基呋喃C3777693C9H14O138.2996.3340.4661.25593
    372-Acetylfuran2-乙酰基呋喃C1192627C6H6O2110.1911.4270.1151.11527
    472-Methylthiophene2-甲基噻吩C554143C5H6S98.2802.7209.2741.04465
    582-Ethylfuran2-乙基呋喃C3208160C6H8O96.1657.5156.281.30748
    酸类69Acetic acid冰醋酸C64197C2H4O260.1588.7140.7261.05139
    743-Methylbutanoic acid异戊酸C503742C5H10O2102.1827.0221.451.21944
    83Dimethyl sulfide二甲基硫C75183C2H6S62.1468.4113.5570.96118
    注:根据软件内置的NIST 2014气相保留指数数据库与 G.A.S.的 IMS 迁移时间数据库,可以明确匹配定性的挥发性物质共63种,包括醛类21 种、醇类13种、酯类10种、酮类9种,烯类3种、杂环类4种,酸类2种及1种硫类。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-08-31
  • 网络出版日期:  2021-05-25
  • 刊出日期:  2021-07-07

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