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中国精品科技期刊2020
秦雪,付世骞,杨鑫焱,等. 重组酶聚合酶等温扩增技术在食源性致病菌检测中的应用[J]. 食品工业科技,2021,42(20):449−455. doi: 10.13386/j.issn1002-0306.2020100136.
引用本文: 秦雪,付世骞,杨鑫焱,等. 重组酶聚合酶等温扩增技术在食源性致病菌检测中的应用[J]. 食品工业科技,2021,42(20):449−455. doi: 10.13386/j.issn1002-0306.2020100136.
QIN Xue, FU Shiqian, YANG Xinyan, et al. Application of Recombinase Polymerase Amplification in Detection of Foodborne Pathogen[J]. Science and Technology of Food Industry, 2021, 42(20): 449−455. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100136.
Citation: QIN Xue, FU Shiqian, YANG Xinyan, et al. Application of Recombinase Polymerase Amplification in Detection of Foodborne Pathogen[J]. Science and Technology of Food Industry, 2021, 42(20): 449−455. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100136.

重组酶聚合酶等温扩增技术在食源性致病菌检测中的应用

Application of Recombinase Polymerase Amplification in Detection of Foodborne Pathogen

  • 摘要: 食品安全是人类面对的重大问题,对食源性致病菌的检测也一直是各种研究关注的重点。基于聚合酶链式扩增(PCR)核酸检测方法虽然已克服传统微生物培养耗时长、准确度不高等缺点,成为目前应用最广的致病菌检测方法,但由于需要精确温度控制大大限制了其在现场检测中的应用。重组酶聚合酶等温扩增技术(RPA),作为一种新兴等温扩增技术,在近十年来发展迅速。该技术打破PCR的壁垒,弥补热循环、需要昂贵仪器等不足,更加适用于资源有限的现场检测。本文总结了重组酶聚合酶等温扩增(RPA)反应机理、引物及探针的设计方法,全面论述RPA在食源性致病菌检测中的应用,概述RPA发展的热点问题并对RPA技术未来发展方向进行展望。

     

    Abstract: Food safety is a major concern for human. Various detection methods for foodborne pathogenic bacteria have become the focus of studies nowadays. Although polymerase chain reaction (PCR), as the most wildly used detection methods for pathogen, has overcome the shortcomings of time-consuming traditional method, the need for accurate temperature control greatly limits its application in field testing. Recombinase polymerase amplification (RPA), as a new isothermal amplification method, has developed rapidly in recent ten years. This method has broken up the barrier of PCR, getting rid of thermal cycle and expensive instruments, which is more suitable for insufficient resources on-site detection. In this manuscript, the mechanism of RPA and the probe design method are provided. Besides, the application of RPA in detecting foodborne pathogenic bacteria is reviewed. The hot topics of RPA development and the prospect of RPA technology development in the future are summarized.

     

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