Abstract: To analyse the characteristic of different microencapsulated Bifidobacterium in simulated digestive tract, different Bifidobacterium were embedded by electrostatic spray, conventional spray and emulsification freezing method. The survival rate, hydrophobicity, copolymerization, adhesion and fatty acid of lipoteichoic acid were studied after passing through the simulated digestive tract. The survival rate of electrostatic spray microencapsulation was higher than traditional spray microencapsulation and emulsification freezing microencapsulation. The survival rate of electrostatic spray microencapsulated Bifidobacterium lactis BL03 was 55.01%±4.12% after passing through the simulated digestive tract.The hydrophobicity of electrostatic spray microencapsulated Bifidobacterium was 125.14%~368.75% higher than emulsification freezing microencapsulation except Bifidobacterium longum BLL2. The copolymerization of electrostatic spray microencapsulated Bifidobacterium was 112.50%~372.72% higher than emulsification freezing microencapsulation. The adhesion of electrostatic spray microencapsulated Bifidobacterium was 109.52%~411.11% higher than emulsification freezing microencapsulation except Bifidobacterium longum BLL2. The Electrostatic spray microencapsulation and traditional spray microencapsulation were better than emulsification freezing microencapsulation. The adhesion and embedding rate was （46.18±2.82）CFU/cell and 93.31%±3.16%, respectively for electrostatic spray microencapsulated Bifidobacterium lactis BL03. The content of C12:0 and C6:0 fatty acid of electrostatic spray microencapsulation and conventional spray microencapsulation were higher than emulsification freezing microencapsulation, but C18:1n9c fatty acid was lower. Electrostatic spray drying was the best microencapsulated method for Bifidobacterium in the condition of simulating digestive tract.