• Scopus
  • CA
  • DOAJ
  • FSTA
  • JST
  • 北大核心期刊
  • 中国科技核心期刊CSTPCD
  • 中国精品科技期刊
  • RCCSE中国核心学术期刊
  • 中国农业核心期刊
  • 中国生物医学文献服务系统SinoMed收录期刊
中国精品科技期刊2020

醋蛋液的醒酒功效及其潜在醒酒功能因子研究

赵梦鸽 张婞 武玉群 侯焘

赵梦鸽,张婞,武玉群,等. 醋蛋液的醒酒功效及其潜在醒酒功能因子研究[J]. 食品工业科技,2021,42(21):370−376. doi:  10.13386/j.issn1002-0306.2020120123
引用本文: 赵梦鸽,张婞,武玉群,等. 醋蛋液的醒酒功效及其潜在醒酒功能因子研究[J]. 食品工业科技,2021,42(21):370−376. doi:  10.13386/j.issn1002-0306.2020120123
ZHAO Mengge, ZHANG Xing, WU Yuqun, et al. Research on the Anti-alcohol Effect of Vinegar Egg Liquid and Its Potential Sobering Functional Factors[J]. Science and Technology of Food Industry, 2021, 42(21): 370−376. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020120123
Citation: ZHAO Mengge, ZHANG Xing, WU Yuqun, et al. Research on the Anti-alcohol Effect of Vinegar Egg Liquid and Its Potential Sobering Functional Factors[J]. Science and Technology of Food Industry, 2021, 42(21): 370−376. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2020120123

醋蛋液的醒酒功效及其潜在醒酒功能因子研究

doi: 10.13386/j.issn1002-0306.2020120123
详细信息
    作者简介:

    赵梦鸽(1995−),女,博士研究生,研究方向:生物活性肽与分子营养,E-mail:zhaomenggehzau@163.com

    通讯作者:

    侯焘(1989−),男,博士,副教授,研究方向:生物活性肽与分子营养,E-mail:houtao@mail.hzau.edu.cn

  • 中图分类号: TS253.9

Research on the Anti-alcohol Effect of Vinegar Egg Liquid and Its Potential Sobering Functional Factors

  • 摘要: 本研究利用小鼠醉酒模型,通过测定小鼠血液中乙醇含量、乙醇脱氢酶(ADH)、谷丙转氨酶(ALT)、谷草转氨酶(AST)的活力探究在不同灌胃剂量及不同灌胃时间下,醋蛋液的醒酒活性,并对醋蛋液进行结构鉴定,厘清醋蛋液发挥醒酒功能的活性因子。结果表明:酒后15 min灌胃54 mL/kg醋蛋液时,其醒酒效果最佳,血醇清除率为21.33%。同剂量下(54 mL/kg)与苹果醋、酸奶、“海王金樽”相比,发现灌胃醋蛋液的小鼠其血醇清除率高于其他对照组。灌胃醋蛋液小鼠的血清乙醇脱氢酶ADH活力显著高于模型组、酸奶组与“海王金樽”组(P<0.05),而其肝脏中ALT、AST活力显著高于“海王金樽”组(P<0.05)。结构鉴定结果表明,醋蛋液的结构序列中含有较多的疏水性氨基酸如Leu、Phe、Ala等,且含有如Leu簇(Leu-Leu)等结构单元。醋蛋液能激活ADH发挥良好的醒酒活性,其作用可能与醋蛋液中含有较多的疏水性短肽和Leu簇短肽有关。
  • 图  1  各组醒酒饮料对酒后小鼠肝脏谷丙转氨酶活力的影响

    Figure  1.  Effect of various sobriety drinks on the liver alanine transaminase activity of mice after alcoholic beverages

    注:不同字母表示差异具有统计意义(P<0.05);图2图3同。

    图  2  各组醒酒饮料对酒后小鼠肝脏谷草转氨酶活力的影响

    Figure  2.  Effect of various sobriety drinks on the liver aspartate aminotransferase activity of mice after alcoholic beverages

    图  3  各组醒酒饮料对酒后小鼠血清乙醇脱氢酶活力的影响

    Figure  3.  Effect of various sobriety drinks on the serum ethanol dehydrogenase activity of mice after alcoholic beverages

    图  4  醋蛋液的总离子流图

    Figure  4.  Total ion chromatogram of vinegar egg liquid

    表  1  醋蛋液灌胃剂量与血醇浓度

    Table  1.   Intragastric dose of vinegar egg liquid and serum alcohol concentration

    组别剂量(mL/kg·bw)血醇浓度(mg/mL)血醇清除率(%)
    空白组
    模型组0.515±0.041a
    醋蛋液0.5瓶270.495±0.073ab3.96
    醋蛋液1.0瓶540.428±0.058c16.97
    醋蛋液1.5瓶810.444±0.053bc13.78
    醋蛋液2.0瓶1080.434±0.064c15.71
    注:不同字母表示差异具有统计意义(P<0.05);表2表3同。
    下载: 导出CSV

    表  2  醋蛋液灌胃时间与血醇浓度

    Table  2.   Intragastric administration time of vinegar egg liquid and serum alcohol concentration

    组别剂量(mL/kg·bw)血醇浓度(mg/mL)血醇清除率(%)
    空白组
    模型组0.506±0.122a
    酒前30 min540.401±0.119b20.70
    酒前15 min540.431±0.062ab14.70
    酒前5 min540.458±0.051ab9.31
    酒后5 min540.404±0.070b20.04
    酒后15 min540.398±0.064b21.33
    酒后30 min540.384±0.056b23.94
    下载: 导出CSV

    表  3  不同醒酒饮料与血醇浓度

    Table  3.   Different sobering-up drinks and serum alcohol concentration

    组别剂量(mL/kg·bw)血醇浓度(mg/mL)血醇清除率(%)
    空白组
    模型组0.487±0.060a
    醋蛋液540.386±0.070b20.82
    苹果醋540.429±0.070ab11.85
    酸奶540.409±0.064b16.03
    海王金樽540.452±0.076ab7.30
    下载: 导出CSV

    表  4  醋蛋液的结构解析结果

    Table  4.   Peptides identified from vinegar egg liquid using nano-LC-MS/MS

    序号肽序列iBAQ序号肽序列iBAQ
    1LPFSIK9207.936MSPHHWYPVR48177.8
    2LPLPPLG7072.737LPENAYLLKVR3240172.9
    3PIQLLK20970.238ADTYFDNYRVG3240172.9
    4IDFLTK5711981.139KDSNVNWNNLK1206666.7
    5DLFILK607728.840IAAEGLQEMFVR1378767.2
    6YSKVIR3240172.941KILDDTDNQATR1378767.2
    7LPLLSPK3029402.142DLLFKDSAIMLK1206666.7
    8ISLTVIK200302.143LLQMPIPLIRVK8602.7
    9IPIPISR66201.344NFHFHTFDHVK75135.6
    10ADVISSLK045LPDMILYQKAVR3240172.9
    11DVHILLK816777.346EILNMAQYQPSR697402.6
    12KLNFILK047IEDLKSHVEEALK697402.6
    13LQEMFVR1378767.248DVIPPGITIVAQAVR1378767.2
    14LSSKLEISG3240172.949VDSKCYSTEPVLR3240172.9
    15FDAKIDVK3240172.950LPLEYGSYTTALAR3240172.9
    16LFLPPYLAG67786.851NVPLYNAIGEHALR1378767.2
    17KSLWDILK697402.652SHEIDMHPVNGQVK3240172.9
    18IEAHVSVKAG697402.653ISQAVHAAHAEINEAG0
    19LPLSLPVGPR3240172.954MSFKPVYSDVPIEK1378767.2
    20LPENAYLLK3240172.955VEGNVIFDPSSYVPK697402.6
    21AQSDFGVDTK1206666.756SELLQLPVHLFKIK1378767.2
    22QQLTLVEVR3240172.957DDNKVEDIWSFLSK1206666.7
    23SDVEKFLLK85215.658DASFIQNTYLHKLIG3240172.9
    24AQIEEVLQR059SFVASHIANILDSDEVG697402.6
    25ALKLMHLLR1378767.260EALQPIHDLADEAISR3240172.9
    26HNRSQEVVK84047.661LYPEKDEPLNVLNLK697402.6
    27QFAAHPAIQVK84047.662NILQNNADKAYMFAAR697402.6
    28QMKLLINLIK063LVLNMPANKNSVPITVR11748.2
    29DSNVNWNNLK1206666.764ASLWIHNENQGFALAAPG3240172.9
    30VEDIWSFLSK1206666.765NVIFDPSSYVPKETMLK697402.6
    31LQELNIQKIK697402.666EILNMAQYQPSRASFYG697402.6
    32AVILNLEKLIK697402.667QVVSMAELIHSDKDLFILK607728.8
    33ILDDTDNQATR1378767.268VEMNSNIFHESGIEAHVSVK697402.6
    34LSLSARTAEVTK607728.869IVLMPVHTDADIDKIQLEIQAG3240172.9
    35STTSYYAVAVVK1206666.7
    下载: 导出CSV
  • [1] 张明媛, 牛俊奇. 东方国家原发性肝癌发病趋势及影响因素[J]. 临床肝胆病杂志,2018,34(7):1399−1402. [Zhang M Y, Niu J Q. Prevalence of primary liver cancer in Eastern countries and related influencing factors[J]. Journal of Clinical Hepatobiliary Diseases,2018,34(7):1399−1402. doi:  10.3969/j.issn.1001-5256.2018.07.007
    [2] 丁荣荣. 壳寡糖对急性酒精性肝损伤的保护作用研究[D]. 无锡: 江南大学, 2019

    Ding R R. Study on hepato protective activity of chitooligosaccharide on acute alcoholicliver disease[D]. Wuxi: Jiangnan University, 2019.
    [3] Sugimoto K, Takei Y. Pathogenesis of alcoholic liver disease[J]. Hepatology Research the Official Journal of the Japan Society of Hepatology,2017,47(1):70−79. doi:  10.1111/hepr.12736
    [4] Teschke R. Alcoholic liver disease: Alcohol metabolism, cascade of molecular mechanisms, cellular targets, and clinical aspects[J]. Biomedicines,2018,6(4):106. doi:  10.3390/biomedicines6040106
    [5] Cederbaum A I. Cytochrome P450 2E1-dependent oxidant stress and upregulation of anti-oxidant defense in liver cells[J]. Journal of Gastroenterology and Hepatology,2006,21(Supplement s3):S22−S25.
    [6] 朱晔, 田会婷, 郭呈斌, 等. 姜黄素-玉米肽复合物的制备及其对小鼠的醒酒保肝作用研究[J]. 河南大学学报:医学版,2019,38(1):16−20. [Zhu Y, Tian H, Guo C, et al. Study on the preparation of curcumin-corn peptide complex and its effect on anti-alcoholism and hepatic-protection[J]. Journal of Henan University:Medical Edition,2019,38(1):16−20.
    [7] 杨建成, 吴高峰, 刘梅, 等. 牛磺酸解酒防醉作用的研究[J]. 安徽农业科学,2012(4):2062−2063. [Yang J C, Wu G F, Liu M, et al. Study on relieving alcoholism and preventing drunkenness effect of taurine[J]. Anhui Agricultural Sciences,2012(4):2062−2063. doi:  10.3969/j.issn.0517-6611.2012.04.053
    [8] 于国才. 新型高活性玉米醒酒保肝肽的制备、活性与结构[D]. 武汉: 华中农业大学, 2010

    Yu G C. Preparation, activity and structures of neotype potent bioactive corn peptides as alcohol metabolism stimulator and hepato protective agent[D]. Wuhan: Huazhong agriculture university, 2010.
    [9] 隋玉杰, 何慧, 王进, 等. 中性蛋白酶及碱性蛋白酶制备玉米粗肽的醒酒活性比较[J]. 中国粮油学报,2006(3):102−106. [Sui Y J, He H, Wang J, et al. Comparison the anti-alcoholism bioactivities between corn crude peptides prepared by neutrase and alcalase[J]. Journal of the Chinese Cereals Oils Association,2006(3):102−106. doi:  10.3321/j.issn:1003-0174.2006.03.023
    [10] 隋玉杰, 何慧, 石燕玲, 等. 玉米肽的醒酒活性体外试验及其醒酒机理研究[J]. 中国粮油学报,2008,23(5):54−58. [Sui Y J, He H, Shi Y L, et al. A study on mechanism and tests in vitro forcorn peptides facilitating alcohol metabolism[J]. Journal of the Chinese Cereals Oils Association,2008,23(5):54−58.
    [11] 郭辉, 何慧, 韩樱, 等. 玉米肽对小鼠酒后肝脏乙醇脱氢酶活力的影响及醒酒机理[J]. 食品科学,2011,32(11):265−269. [Guo H, He H, Han Y, et al. Effect of corn peptides on alcohol dehydrogenase activity in live of mice after drinking and its anti-alcohol mechanism[J]. Food Science,2011,32(11):265−269.
    [12] 马芝丽. 玉米肽对酒精致肝细胞凋亡干预分子机制及QLLPF的代谢、醒酒构效关系研究[D]. 武汉: 华中农业大学, 2015

    Ma Z L. Mechanism of corn peptides on hepatocyte apoptosis indced by ethanol, metabolism and structure-activity relationship of QLLPF[D]. Wuhan: Huazhong Agriculture University, 2015.
    [13] 王学英, 丁淑娟, 范玉婷. 醋蛋液的成分及保健功效研究进展[J]. 中国调味品,2013,38(5):7−11. [Wang X Y, Ding S J, Fan Y T. Research progress of composition and health function of vinegar egg-juice[J]. China Condiment,2013,38(5):7−11. doi:  10.3969/j.issn.1000-9973.2013.05.003
    [14] 刘华桥, 李清逸, 李双艳, 等. 醋蛋液解酒功效的研究[J]. 食品安全导刊,2019(12):65−68. [Liu H Q, Li Q Y, Li S Y, et al. Study on antialcoholism effect of vinegar egg solution[J]. China Food Safety Magazine,2019(12):65−68.
    [15] Wu Y, He H, Hou T. Purification, identification, and computational analysis of xanthine oxidase inhibitory peptides from kidney bean[J]. Journal of Food Science,2021.
    [16] 张自然. 牡蛎肉的醒酒作用及其醒酒机理的研究[D]. 湛江: 广东海洋大学, 2009

    Zhang Z R. Study on the anti-alcoholism and mechanism of Oyster meat[D]. Zhanjiang: Guangdong Ocean University, 2009
    [17] 杜双奎, 赵晓野, 李志西. 枳椇醋解酒护肝、减肥降脂作用研究[J]. 食品科学,2012(1):235−238. [Du S K, Zhao X Y, Li Z X. Hepatoprotective, weight-reducing and hypolipidemic effects of Hovenia dulcis thunb. fruit vinegar[J]. Food Science,2012(1):235−238.
    [18] 杜鹏, 赵亚东, 穆莹, 等. 解酒酸乳饮料的生产及功效[J]. 中国乳品工业,2010,38(1):26−28. [Du P, Zhao Y D, Mu Y, et al. Production and function of dealcohol yogurt beverage[J]. China Dairya Industry,2010,38(1):26−28. doi:  10.3969/j.issn.1001-2230.2010.01.007
    [19] 张效敏, 芦瑞萍, 海秀玲, 等. 浅析血清谷丙转氨酶水平与肝脏病理改变的关系[J]. 医药前沿,2019,9(11):32−34. [Zhang X M, Lu R P, Hai X L, et al. Analysis of the relationship between serum alanine aminotransferase level and liver pathological changes[J]. Medical Frontier,2019,9(11):32−34.
    [20] 钟锐, 张俊. 肝功能指标在原发性肝癌中的诊断价值[J]. 中国现代医学杂志,2015,25(8):102−105. [Zhong R, Zhang J. Diagnostic value of common biochemical indicators of liver function for primary hepatocellular cancer[J]. China Journal of Modern Medicine,2015,25(8):102−105. doi:  10.3969/j.issn.1005-8982.2015.08.025
    [21] 李旭, 苑隆国, 王晓辉. 牡蛎提取物对小鼠肝脏保护作用研究[J]. 医学研究通讯,2005,34(1):51−52. [Li Xu, Yuan L G, Wang X H. Protective effect of oyster extract on mouse liver[J]. Medical Research Newsletter,2005,34(1):51−52.
    [22] Laniewska D M, Jelski W, Orywal K, et al. The activity of class I, II, III and IV of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in brain cancer[J]. Neurochemical Research,2013,38(7):1517−1521. doi:  10.1007/s11064-013-1053-9
    [23] Cederbaum A I. Alcohol metabolism[J]. Clinics in Liver Disease,2012,16(4):667−685. doi:  10.1016/j.cld.2012.08.002
    [24] Guo C Y, Jiang T L, Hui H, et al. Ultrafiltration preparation of potent bioactive corn peptide as alcohol metabolism stimulator in vivo and study on its mechanism of action[J]. Journal of Food Biochemistry,2013,37(2):161−167. doi:  10.1111/j.1745-4514.2011.00613.x
    [25] Wang C, He H, Zhang J L, et al. High performance liquid chromatography (HPLC) fingerprints and primary structure identification of corn peptides by HPLC-diode array detection and HPLC-electrospray ionization tandem mass spectrometry[J]. Journal of Food & Drug Analysis,2016,24(1):95−104.
    [26] 韩光顺, 梁华益, 韦家河, 等. 茴香提取液对小鼠酒精性肝损伤的保护作用[J]. 现代预防医学,2017(18):58−62. [Hang G S, Liang H Y, Wei J H. Protective effect of fennel extract on alcoholic liver injury in mice[J]. Modern Preventive Medicine,2017(18):58−62.
    [27] Shimizu M, Tsunogai M, Arai S. Transepithelial transport of oligopeptides in the human intestinal cell, Caco-2[J]. Peptides,1997,18(5):681−687. doi:  10.1016/S0196-9781(97)00002-8
    [28] 赵梦鸽, 何慧, 马爱民, 等. 蛋清源生物活性肽功能及其构效关系研究进展[J]. 华中农业大学学报,2020:1−9. [Zhao M G, He H, Ma A M, et al. Bioactive peptides derived from duck and chicken egg white proteins: A review[J]. Journal of Huazhong Agricultural University,2020:1−9.
    [29] Yamaguchi M, Takada M, Nozaki O, et al. Preparation of corn peptide from corn gluten meal and its administration effect on alcohol metabolism in stroke-prone spontaneously hypertensive rats[J]. Journal of Nutritional ence & Vitaminology,1996,42(3):219−231.
    [30] Rajapakse N, Mendis E, Jung W K, et al. Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties[J]. Food Research International,2005,38(2):175−182. doi:  10.1016/j.foodres.2004.10.002
    [31] Chen C, Dickman M B. Proline suppresses apoptosis in the fungal pathogen colletotrichum trifolii[J]. Proceedings of the National Academy of Sciences of the United States of America,2005,102(9):3459−3464.
  • 加载中
图(4) / 表(4)
计量
  • 文章访问数:  896
  • HTML全文浏览量:  33
  • PDF下载量:  28
  • 被引次数: 0
出版历程
  • 收稿日期:  2020-12-16
  • 网络出版日期:  2021-09-21
  • 刊出日期:  2021-10-25

目录

    /

    返回文章
    返回

    重要通知

    1 权威:2022年度食品技术创新奖、产品创新奖发布

     2 喜讯: 《食品工业科技》被DOAJ收录

     3 快速见刊:客座主编专栏征稿-食源性功能物质挖掘及评价