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中国精品科技期刊2020
丁政宇,张士凯,何子杨,等. 响应面优化黄精渣不溶性膳食纤维酶法提取工艺及其结构表征[J]. 食品工业科技,2021,42(20):157−163. doi: 10.13386/j.issn1002-0306.2021010094.
引用本文: 丁政宇,张士凯,何子杨,等. 响应面优化黄精渣不溶性膳食纤维酶法提取工艺及其结构表征[J]. 食品工业科技,2021,42(20):157−163. doi: 10.13386/j.issn1002-0306.2021010094.
DING Zhengyu, ZHANG Shikai, HE Ziyang, et al. Optimization of Enzymatic Extraction Process of Insoluble Dietary Fiber from Polygonatum sibiricum Residue by Response Surface Methodology and Its Characterization[J]. Science and Technology of Food Industry, 2021, 42(20): 157−163. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010094.
Citation: DING Zhengyu, ZHANG Shikai, HE Ziyang, et al. Optimization of Enzymatic Extraction Process of Insoluble Dietary Fiber from Polygonatum sibiricum Residue by Response Surface Methodology and Its Characterization[J]. Science and Technology of Food Industry, 2021, 42(20): 157−163. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010094.

响应面优化黄精渣不溶性膳食纤维酶法提取工艺及其结构表征

Optimization of Enzymatic Extraction Process of Insoluble Dietary Fiber from Polygonatum sibiricum Residue by Response Surface Methodology and Its Characterization

  • 摘要: 以黄精渣为原料,采用响应面优化酶法提取黄精渣不溶性膳食纤维(HIDF)。通过单因素实验研究料液比、木瓜蛋白酶浓度、木瓜蛋白酶酶解时间、α-淀粉酶浓度、α-淀粉酶酶解时间五种因素对HIDF得率的影响,并采用响应面(Box-Behnken)优化提取工艺参数;最后进行扫描电镜(SEM)、红外光谱分析(FTIR)和X射线衍射(XRD)观察以及功能性质测定。结果表明,最佳提取工艺为:料液比1:20 g/mL,木瓜蛋白酶浓度0.13%,木瓜蛋白酶酶解时间1.9 h,α-淀粉酶浓度0.29%,α-淀粉酶酶解时间2 h,HIDF得率为52.18%;HIDF的持水力、持油力和膨胀力分别为5.99±0.05 g/g、3.97±0.04 g/g和4.57±0.05 mL/g。综上,提取后的黄精膳食纤维具有较好的结构及物理性质,适合加工成功能性食品,可为提高黄精渣的利用率及进一步挖掘营养价值提供参考。

     

    Abstract: The insoluble dietary fiber (HIDF) was extracted from Polygonatum sibiricum residue by response surface methodology. Firstly, the effects of solid-liquid ratio, papain concentration, papain hydrolysis time, α-amylase concentration and α-amylase hydrolysis time on the extraction rate of HIDF were discussed by single factor experiments, the extraction process parameters were optimized by response surface methodology (Box-Behnken). Finally, infrared spectrum analysis, X-ray diffraction and scanning electron microscope observation were carried out, the functional properties were determined. The results showed that the optimum extraction conditions were as follows: Solid-liquid ratio 1:20 mL/g, papain concentration 0.13%, papain enzymolysis time 1.9 h, α-amylase concentration 0.29%, α-amylase hydrolysis time 2 h, the content of HIDF was 52.184%. The hydraulic holding capacity, oil holding capacity and swelling capacity of HIDF were 5.99±0.05 g/g, 3.965±0.04 g/g and 4.565±0.05 mL/g, respectively. Therefore, HIDF extracted from Polygonatum sibiricum residue had good physical and chemical properties and was suitable for processing into functional food, which provided a reference for improving the utilization rate of Polygonatum sibiricum residue and further excavating its nutritional value.

     

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