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中国精品科技期刊2020
张楠,钱滢文,吴丽华,等. 基于离子液体柱前荧光衍生高效液相色谱法测定动物性食品中7种β-受体激动剂[J]. 食品工业科技,2021,42(23):296−302. doi: 10.13386/j.issn1002-0306.2021030237.
引用本文: 张楠,钱滢文,吴丽华,等. 基于离子液体柱前荧光衍生高效液相色谱法测定动物性食品中7种β-受体激动剂[J]. 食品工业科技,2021,42(23):296−302. doi: 10.13386/j.issn1002-0306.2021030237.
ZHANG Nan, QIAN Yingwen, WU Lihua, et al. Detection of 7 β-agonists in Animal-derived Food based on Ionic Liquid and Pre-column Fluorescence Derivatization-High Performance Liquid Chromatography[J]. Science and Technology of Food Industry, 2021, 42(23): 296−302. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030237.
Citation: ZHANG Nan, QIAN Yingwen, WU Lihua, et al. Detection of 7 β-agonists in Animal-derived Food based on Ionic Liquid and Pre-column Fluorescence Derivatization-High Performance Liquid Chromatography[J]. Science and Technology of Food Industry, 2021, 42(23): 296−302. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030237.

基于离子液体柱前荧光衍生高效液相色谱法测定动物性食品中7种β-受体激动剂

Detection of 7 β-agonists in Animal-derived Food based on Ionic Liquid and Pre-column Fluorescence Derivatization-High Performance Liquid Chromatography

  • 摘要: 以荧光衍生剂2-(4-磺酰氯基苯基)苯并咪唑和7种β-受体激动剂发生反应,达到增强7种β-受体激动剂荧光强度的目的,建立了动物性食品中7种β-受体激动剂的高效液相色谱-荧光检测方法。研究了碳酸盐缓冲溶液pH、反应温度、反应时间和2-(4-磺酰氯基苯基)苯并咪唑的浓度对衍生反应的影响。结果表明:在最优实验条件下,7种β-受体激动剂在1.0~100.0 ng/mL范围内呈良好的线性关系,线性相关系数R2>0.999,检出限约为0.30 μg/kg,定量限<1.0 μg/kg。空白猪肉、羊肉、牛肉样品在1.0、10.0、50.0 μg/kg这三个不同浓度水平进行加标实验,回收率分别为81.3%~107.3%、80.5%~110.7%和80.2%~105.1%,日内相对标准偏差分别为1.48%~13.51%、1.26%~12.33%和1.23%~11.49%,日间相对标准偏差分别为2.21%~11.54%、1.82%~13.31%和1.07%~13.21%。该方法精密度好、准确度高,抗干扰能力强,适合动物性食品中7种β-受体激动剂残留量的定性确证分析和定量分析。

     

    Abstract: In order to enhance the fluorescence intensity of 7 β-agonists, the fluorescent derivatizer 2-(4-sulfoyl chloro-phenyl) benzimidazole reacted with β-agonists, a high performance liquid chromatography-fluorescence detection method for 7 β-agonists in animal-derived food was established. Effects of pH of carbonate buffer solution, reaction temperature, reaction time and concentration of 2-(4-sulfoyl chloro-phenyl) benzimidazole on the fluorescence derivatization were investigated. The results showed that 7 β-agonists had a good linearity in the range of 1.0~100.0 ng/mL and the correlation coefficient (R2) was higher than 0.999 under the optimal conditions. The limits of detection for 7 β-agonists were about 0.30 μg/kg, and the limits of quantitation were less than 1.0 μg/kg. The recoveries of blank pork, mutton and beef samples at three different concentrations of 1.0, 10.0 and 50.0 μg/kg were 81.3%~107.3%, 80.5%~110.7% and 80.2%~105.1%, the intra-day and the inter-day relative standard deviations were 1.48%~13.51%, 1.26%~12.33%, 1.23%~11.49% and 2.21%~11.54%, 1.82%~13.31%, 1.07%~13.21%, respectively. Therefore, the developed method is suitable for determination of 7 β-agonists in animal-derived food qualitatively and quantitatively with satisfactory sensitivity, selectivity, accuracy and precision.

     

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