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佩兰黄酮的纯化及其抗氧化与抗运动疲劳作用

胡华

胡华. 佩兰黄酮的纯化及其抗氧化与抗运动疲劳作用[J]. 食品工业科技,2022,43(15):220−226. doi:  10.13386/j.issn1002-0306.2021110064
引用本文: 胡华. 佩兰黄酮的纯化及其抗氧化与抗运动疲劳作用[J]. 食品工业科技,2022,43(15):220−226. doi:  10.13386/j.issn1002-0306.2021110064
HU Hua. Purification of Flavonoids from Eupatorium fortunei Turcz. and Its Anti-oxidant and Resisting Exercise Fatigue Effects[J]. Science and Technology of Food Industry, 2022, 43(15): 220−226. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021110064
Citation: HU Hua. Purification of Flavonoids from Eupatorium fortunei Turcz. and Its Anti-oxidant and Resisting Exercise Fatigue Effects[J]. Science and Technology of Food Industry, 2022, 43(15): 220−226. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2021110064

佩兰黄酮的纯化及其抗氧化与抗运动疲劳作用

doi: 10.13386/j.issn1002-0306.2021110064
基金项目: 浅谈在现代体育教学中“运动负荷”的应用(WLYB2021034)。
详细信息
    作者简介:

    胡华(1982−),女,本科,副教授,研究方向:体育训练与营养,E-mail:huhua19820704@163.com

  • 中图分类号: TS201.2

Purification of Flavonoids from Eupatorium fortunei Turcz. and Its Anti-oxidant and Resisting Exercise Fatigue Effects

  • 摘要: 研究大孔树脂纯化佩兰黄酮提取物的最佳条件及其抗氧化与抗运动疲劳活性。采用单因素实验确定该树脂纯化佩兰黄酮提取物的最佳条件,并通过体外抗氧化实验和动物实验研究不同产物的抗氧化与抗运动疲劳活性。结果表明,最佳纯化条件:上样浓度与流速分别为3.0 mg/L和1.0 mL/min,上样液pH与体积分别为4.0与60 mL,洗脱液乙醇体积分数与体积分别为70%与100 mL,洗脱流速为1.0 mL/min,产物的黄酮纯度由24.2%增大至69.4%。纯化后的佩兰黄酮对羟基自由基(∙OH)与超氧阴离子自由基(O2∙)的半抑制浓度(IC50)分别为0.47、0.42 mg/mL,抗氧化能力增强,可明显延长小鼠的运动时间,降低其运动后乳酸与尿素氮浓度,并有助于增强SOD与GSH-Px的活力,从而减少丙二醛的生成,因此具有较好的抗运动疲劳活性,可为佩兰黄酮利用提供参考。
  • 图  1  上样液浓度对佩兰黄酮的吸附率影响

    Figure  1.  Effect of different loading solution concentration on adsorption rate of Eupatorium fortunei Turcz. flavonoids

    注:不同小写字母表示差异显著(P<0.05),图2、图4、图6~图7。

    图  2  上样液pH对佩兰黄酮的吸附率影响

    Figure  2.  Effect of different pH value of loading solution onadsorption rate of Eupatorium fortunei Turcz. flavonoids

    图  3  不同流速下大孔树脂的吸附泄漏曲线

    Figure  3.  Adsorption leakage curve of the macroporous resin in different flow rate

    图  4  不同体积分数的乙醇溶液对洗脱率的影响

    Figure  4.  Effect of different ethyl alcohol concentration on elution rate

    图  5  不同流速下大孔树脂的洗脱曲线

    Figure  5.  Elution curve of the macroporous resin at different flow rate

    图  6  不同物质对∙OH的清除能力

    Figure  6.  Scavenging ability of different substances on hydroxyl radical

    图  7  不同物质对O2∙的清除能力

    Figure  7.  Scavenging ability of different substances on superoxide anion radical

    表  1  不同组小鼠的爬杆时间 (n=10)

    Table  1.   The climbing time in mice of different groups (n=10)

    小组爬杆时间(min)平均延长时间(min)
    空白对照组12.7±0.85
    阳性对照组17.5±0.92**##4.8
    提取组13.6±1.05*1.2
    纯化组14.7±0.95**##2.4
    注:与空白对照组比较,*表示显著性差异,P<0.05,**表示极显著差异,P<0.01;与提取组比较,#表示显著性差异,P<0.05,##表示极显著差异,P<0.01。表2~表3同。
    下载: 导出CSV

    表  2  不同组小鼠的LA与BUN浓度(n=10)

    Table  2.   The contents of LA and BUN in mice of different groups (n=10)

    小组LA(mmol/L)BUN(mmol/L)
    空白对照组13.15±1.048.37±0.45
    阳性对照组9.82±0.72**##6.56±0.52**##
    提取组11.98±1.12*7.76±0.59*
    纯化组10.72±0.93**#7.03±0.71**#
    下载: 导出CSV

    表  3  不同组小鼠的MDA浓度和SOD、GSH-Px活性(n=10)

    Table  3.   MDA content and SOD, GSH-Px activity in mice of different groups (n=10)

    小组MDA(mmol/L)SOD(U/mL)GSH-Px(U/mL)
    空白对照组3.62±0.25177.25±25.16473.71±35.37
    阳性对照组2.46±0.29**##236.15±29.28**##555.71±33.68**##
    提取组3.31±0.37*199.52±21.43*498.35±27.44*
    纯化组2.88±0.22**##219.46±18.69**#528.16±23.69**##
    下载: 导出CSV
  • [1] 陈慧, 马璇, 曹丽行, 等. 运动疲劳机制及食源性抗疲劳活性成分研究进展[J]. 食品科学,2020,41(11):247−258. [CHEN H, MA X, CAO L X, et al. Recent progress in the mechanism behind exercise-induced fatigue and dietary bioactive components against fatigue[J]. Food Science,2020,41(11):247−258. doi:  10.7506/spkx1002-6630-20190620-235

    CHEN H, MA X, CAO L X, et al. Recent progress in the mechanism behind exercise-induced fatigue and dietary bioactive components against fatigue[J]. Food Science, 2020, 41(11): 247-258. doi:  10.7506/spkx1002-6630-20190620-235
    [2] BLOCH W, ZOPF E, ZIMMER P, et al. Role of physical activity in tumor patients and possible underlying mechanisms[J]. European Review of Aging and Physical Activity,2013,10(1):25−32. doi:  10.1007/s11556-012-0106-4
    [3] BOSCÃ L, ARAGÃ N J J, SOLS A. Modulation of muscle phosphofructokinase at physiological concentration of enzyme[J]. Journal of Biological Chemistry,1985,260(4):2100−2107. doi:  10.1016/S0021-9258(18)89522-9
    [4] MA S, SUZUKI K. Keto-adaptation and endurance exercise capacity, fatigue recovery, and exercise-induced muscle and organ damage prevention: A narrative review[J]. Sports,2019,7(2):40−49. doi:  10.3390/sports7020040
    [5] 雷培培. 短梗五加多酚运动饮料制备及缓解疲劳作用[J]. 食品工业科技,2021,42(11):174−179. [LEI P P. Preparation of polyphenols of Acanthopanax sessiliflorus sports beverage and its fatigue relieving effect[J]. Science and Technology of Food Industry,2021,42(11):174−179.

    LEI P P. Preparation of polyphenols of Acanthopanax sessiliflorus sports beverage and its fatigue relieving effect[J]. Science and Technology of Food Industry, 2021, 42(11): 174-179.
    [6] WANG J, SUN C, ZHENG Y, et al. The effective mechanism of the polysaccharides from Panax ginseng on chronic fatigue syndrome[J]. Archives of Pharmacal Research,2014,37(4):530−538. doi:  10.1007/s12272-013-0235-y
    [7] 谢飞飞. 远志多糖对力竭运动小鼠体内抗疲劳和体外抗氧化作用研究[J]. 食品工业科技,2021,42(6):332−336. [XIE F F. Effects of anti-fatigue in vivo and anti-oxidant in vitro of polysaccharides from Polygala tenuifolia Willd. on exhaustive exercise mice[J]. Science and Technology of Food Industry,2021,42(6):332−336.

    XIE F F. Effects of anti-fatigue in vivo and anti-oxidant in vitro of polysaccharides from Polygala tenuifolia Willd. on exhaustive exercise mice[J]. Science and Technology of Food Industry, 2021, 42(6): 332-336.
    [8] 刘飞, 徐佳馨, 颜娓娓, 等. 中药佩兰体外抗病毒有效部位筛选[J]. 暨南大学学报(自然科学与医学版),2018,39(1):80−86. [LIU F, XU J X, YAN W W, et al. Screen of effective antiviral parts of Chinese medicine Eupatorium fortunei Turcz. in vitro[J]. Journal of Jinan University (Natural Science & Medicine Edition),2018,39(1):80−86.

    LIU F, XU J X, YAN W W, et al. Screen of effective antiviral parts of Chinese medicine Eupatorium fortunei Turcz. in vitro[J]. Journal of Jinan University (Natural Science & Medicine Edition), 2018, 39(1): 80-86.
    [9] 李旭冉, 王梦溪, 朱邵晴, 等. 不同干燥方式对佩兰药材挥发性成分的影响与评价[J]. 中药材,2016,39(12):2747−2752. [LI X R, WANG M X, ZHU S Q, et al. Effects of drying methods on volatile components in Eupatorium fortunei Aerial Part[J]. Journal of Chinese Medicinal Materials,2016,39(12):2747−2752.

    LI X R, WANG M X, ZHU S Q, et al. Effects of drying methods on volatile components in Eupatorium fortunei Aerial Part[J]. Journal of Chinese Medicinal Materials, 2016, 39(12): 2747-2752.
    [10] 许冰. 泽兰与佩兰的化学成分研究[D]. 沈阳: 辽宁师范大学, 2016.

    XU Bing. Study on chemical constituents of Eupatorium adenophorum and Eupatorium fortunei Turez[D]. Shenyang: Liaoning Normal University, 2016.
    [11] 刘杰, 金岩. 佩兰中黄酮类化合物的提取及抑菌活性研究[J]. 上海化工,2012,37(1):15−17. [LIU J, JIN Y. Study on extracting condition of flavonoids from Eupatorium fortunei Turez and its antibacterial activities[J]. Shanghai Chemical Industry,2012,37(1):15−17. doi:  10.3969/j.issn.1004-017X.2012.01.006

    LIU J, JIN Y. Study on extracting condition of flavonoids from Eupatorium fortunei Turez and its antibacterial activities[J]. Shanghai Chemical Industry, 2012, 37(1): 15-17. doi:  10.3969/j.issn.1004-017X.2012.01.006
    [12] 杨观兰, 钟朝玲, 卢连香, 等. 南酸枣叶总黄酮的纯化及其抗氧化活性研究[J]. 食品科技,2021,46(8):178−183. [YANG G L, ZHONG C L, LU L X, et al. Purifcation and antioxidant activity of flavonoids from Choerospondias axillaris leaves[J]. Food Science and Technology,2021,46(8):178−183.

    YANG G L, ZHONG C L, LU L X, et al. Purifcation and antioxidant activity of flavonoids from Choerospondias axillaris leaves[J]. Food Science And Technology, 2021, 46(8): 178-183.
    [13] TANG X, YANG Z, ZHANG J, et al. Synthesis, structure, antioxidation and DNA-binding studies of a zinc(II) complex with tris (2-benzimidazylmethyl) amine and cinnamate[J]. Research on Chemical Intermediates,2015,41(7):4349−4360. doi:  10.1007/s11164-014-1534-8
    [14] CHAE G Y, HA B J. The comparative evaluation of fermented and non-fermented soybean extract on antioxidation and whitening[J]. Toxicological Research,2011,27(4):205−209. doi:  10.5487/TR.2011.27.4.205
    [15] TAN W, YU K Q, LIU Y Y, et al. Antifatigue activity of poly-saccharides extract from Radix Rehmanniae Preparat[J]. International Journal of Biological Macromolecules,2012,50(1):59−62. doi:  10.1016/j.ijbiomac.2011.09.019
    [16] WANG J, LI S S, FAN Y Y, et al. Anti-fatigue activity of the water-soluble polysaccharides isolated from Panax ginseng C. A. Meyer[J]. J Ethnopharmacol,2010,130(2):421−423. doi:  10.1016/j.jep.2010.05.027
    [17] 王书全, 李丽. 螺旋藻多糖抗疲劳作用研究[J]. 食品工业科技,2013,34(22):328−330. [WANG S Q, LI L. Study on anti-fatigue effect of Spirulina polysaccharide[J]. Science and Technology of Food Industry,2013,34(22):328−330.

    WANG S Q, LI L. Study on anti-fatigue effect of Spirulina polysaccharide[J]. Science and Technology of Food Industry, 2013, 34(22): 328-330.
    [18] 邓云兵, 黄冬琴, 岳天翔. 淡竹叶多糖的大孔吸附树脂纯化工艺及对小鼠运动耐力的影响[J]. 食品工业科技,2021,42(14):169−174. [DENG Y B, HUANG D Q, YUE T X. Purification technology of polysaccharides from Lophatherum gracile Brongn. by macroporous resin adsorption and its effect on athletic endurance of mice[J]. Science and Technology of Food Industry,2021,42(14):169−174.

    DENG Y B, HUANG D Q, YUE T X. Purification technology of polysaccharides from Lophatherum gracile Brongn. by macroporous resin adsorption and its effect on athletic endurance of mice[J]. Science and Technology of Food Industry, 2021, 42(14): 169-174.
    [19] 刘大伟. 黄芩多糖大孔树脂纯化工艺优化及其抑菌活性[J]. 食品工业科技,2021,42(15):183−188. [LIU D W. Optimization purification process of polysaccharides from Scutellaria baicalensis Georgi by macroporous resins and its antibacterial activity[J]. Science and Technology of Food Industry,2021,42(15):183−188.

    LIU D W. Optimization purification process of polysaccharides from Scutellaria baicalensis Georgi by macroporous resins and its antibacterial activity[J]. Science and Technology of Food Industry, 2021, 42(15): 183-188.
    [20] 张沛, 吴楠, 宋志军, 等. 响应面法优化大孔树脂纯化黄芪毛蕊异黄酮工艺[J]. 食品工业科技,2021,42(10):209−214. [ZHANG P, WU N, SONG Z J, et al. Purification of calycosin from Astragalusme mbranceus with macroporous resins by response surface analysis[J]. Science and Technology of Food Industry,2021,42(10):209−214.

    ZHANG P, WU N, SONG Z J, et al. Purification of calycosin from Astragalusme mbranceus with macroporous resins by response surface analysis[J]. Science and Technology of Food Industry, 2021, 42(10): 209-214.
    [21] 韩秋菊, 李薇, 冯雅郡, 等. 藁本黄酮的纯化及体外抗氧化活性研究[J]. 应用化工,2021,50(7):1863−1866. [HAN Q J, LI W, FENG Y J, et al. Purification and antioxidant activity of Ligusticum flavonoids in vitro[J]. Applied Chemical Industry,2021,50(7):1863−1866. doi:  10.3969/j.issn.1671-3206.2021.07.027

    HAN Q J, LI W, FENG Y J, et al. Purification and antioxidant activity of Ligusticum flavonoids in vitro[J]. Applied Chemical Industry, 2021, 50(7): 1863-1866. doi:  10.3969/j.issn.1671-3206.2021.07.027
    [22] 李倩, 奥斯曼江·麦提图尔荪, 李晨阳, 等. 大孔树脂纯化新疆圆柏总黄酮工艺研究[J]. 食品工业科技,2016,37(7):188−192. [LI Q, MAITITUERSUN A, LI C Y, et al. Purification of total flavonoids from Junipers sabina L. by macroporous resin[J]. Science and Technology of Food Industry,2016,37(7):188−192.

    LI Q, MAITITUERSUN A, LI C Y, et al. Purification of total flavonoids from Junipers sabina L. by macroporous resin[J]. Science and Technology of Food Industry, 2016, 37(7): 188-192.
    [23] MASON S A, MORRISON D, MCCONELL G K, et al. Muscle redox signalling pathways in exercise. Role of antioxidants[J]. Free Radical Biology & Medicine,2016,98:29−45.
    [24] YANG D S, LIAN J H, WANG L Y, et al. The anti-fatigue and anti-anoxia effects of Tremella extract[J]. Saudi Journal of Biological Sciences,2019,26(8):2052−2056. doi:  10.1016/j.sjbs.2019.08.014
    [25] YOU L, ZHAO M, REGENSTEIN J M, et al. In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion[J]. Food Chemistry,2011,124(1):188−194. doi:  10.1016/j.foodchem.2010.06.007
    [26] TRINITY J D, BROXTERMAN R M, RICHARDSON R S. Regulation of exercise blood flow: Role of free radicals[J]. Free Radical Biology and Medicine,2016,98:90−102. doi:  10.1016/j.freeradbiomed.2016.01.017
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  • 收稿日期:  2021-11-08
  • 网络出版日期:  2022-06-19
  • 刊出日期:  2022-08-03

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