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LI Xiong, GUO Xing, YU Pei, XU Hui, LIN Song- yi. Secondary structure of MMCTN treated by pulsed electric field( PEF) technology[J]. Science and Technology of Food Industry, 2016, (03): 74-78. doi: 10.13386/j.issn1002-0306.2016.03.006
Citation: LI Xiong, GUO Xing, YU Pei, XU Hui, LIN Song- yi. Secondary structure of MMCTN treated by pulsed electric field( PEF) technology[J]. Science and Technology of Food Industry, 2016, (03): 74-78. doi: 10.13386/j.issn1002-0306.2016.03.006

Secondary structure of MMCTN treated by pulsed electric field( PEF) technology

doi: 10.13386/j.issn1002-0306.2016.03.006
  • Received Date: 2015-06-03
  • DPPH radical scavenging capacity was used as measuring indices of antioxidant activity In this research.Two- factor- at- a- time experiment was used to investigate the pulsed electric field( PEF) effects on antioxidant activity of MMCTN and the two factors were electric filed intensity( 5,10,15,20 k V / cm) and pulse frequency( 1800,2400 Hz).And the effects of retention time( 0,2 h) after PEF treatment on antioxidant activity were investigated.Then,mid- infrared spectroscopy( MIR) and circular dichroism spectrum( CD) were used to analyze the secondary structure changes of PEF treated MMCTN.The results showed that when the electric filed intensity was 10 k V / cm,pulse frequency was 2400 Hz and retention time was 2 h,the DPPH radical scavenging capacity was up to 94.14%± 0.13%.The MIR showed that the functional groups of PEF treated samples were changed and the CD indicated that α helices,β strands,β turn and random coil were interconverted.
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