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ZENG Cheng, MA Mao-mao, XIAO Yan-jun, ZENG Zhe-ling, YU Ping, ZHONG Wei-min. Culture optimizations and application for Bacillus amyloliquefaciens Z16 to produce neutral protease[J]. Science and Technology of Food Industry, 2016, (03): 196-200. doi: 10.13386/j.issn1002-0306.2016.03.033
Citation: ZENG Cheng, MA Mao-mao, XIAO Yan-jun, ZENG Zhe-ling, YU Ping, ZHONG Wei-min. Culture optimizations and application for Bacillus amyloliquefaciens Z16 to produce neutral protease[J]. Science and Technology of Food Industry, 2016, (03): 196-200. doi: 10.13386/j.issn1002-0306.2016.03.033

Culture optimizations and application for Bacillus amyloliquefaciens Z16 to produce neutral protease

doi: 10.13386/j.issn1002-0306.2016.03.033
  • Received Date: 2015-04-23
  • The enzyme- producing conditions of Bacillus amyloliquefaciens Z16 and the technological conditions of aqueous enzymatic extraction of camphor tree seed kernel oil were optimized by using single- factor experiment and orthogonal experiment,meanwhile,compared with the Bacillus subtilis AS1.398.The best fermentation medium composition of Bacillus amyloliquefaciens Z16 was corn meal 4.5%,wheat bran 2.5%,soya bean meal 4.0%,Ca Cl20.2%,KH2PO4 0.03%,Na2HPO4· 12H2 O 0.4%,p H7.5,and the best fermentation conditions of Bacillus amyloliquefaciens Z16 were inoculation amount 2%,temperature 37 ℃,rate 220 r / min,shake flask culture 44 h.Under the best enzyme- producing technological conditions,the activity of neutral protease produced by Bacillus amyloliquefaciens Z16 was 6984.3 U / m L,which was raised by 54.0% after the optimization.The optimum conditions of of Bacillus amyloliquefaciens Z16 aqueous enzymatic extraction of camphor tree seed kernel oil were enzyme adding proportion 20%( v / v),enzymolysis time 4 h. The corresponding camphor tree seed kernel oil yield was91.1%,and its acid value only rose by 0.3 mg KOH / m L. The effect of Bacillus amyloliquefaciens Z16 on aqueous enzymatic extraction of camphor tree seed kernel oil was obviously superior to Bacillus subtilis AS1.398 commonlyused to produce neutral protease.
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