Study on the preparation technology of hemin from enzymolysis yak blood by papain

JIA Zhi- chun; ZHANG Zhen; ZHANG Sheng-gui; NIU Li-li; ZHAO Yuan-yuan; XIAO Xue- li; XU Rong- rong; BAO Xue- mei

doi:10.13386/j.issn1002-0306.2016.03.035

Article Contents

Article Navigation > Science and Technology of Food Industry > 2016 > (03): 206-210

JIA Zhi- chun, ZHANG Zhen, ZHANG Sheng-gui, NIU Li-li, ZHAO Yuan-yuan, XIAO Xue- li, XU Rong- rong, BAO Xue- mei. Study on the preparation technology of hemin from enzymolysis yak blood by papain[J]. Science and Technology of Food Industry, 2016, (03): 206-210. doi: 10.13386/j.issn1002-0306.2016.03.035

Citation:

JIA Zhi- chun, ZHANG Zhen, ZHANG Sheng-gui, NIU Li-li, ZHAO Yuan-yuan, XIAO Xue- li, XU Rong- rong, BAO Xue- mei. Study on the preparation technology of hemin from enzymolysis yak blood by papain[J]. Science and Technology of Food Industry, 2016, (03): 206-210. doi: 10.13386/j.issn1002-0306.2016.03.035

Citation:

JIA Zhi- chun, ZHANG Zhen, ZHANG Sheng-gui, NIU Li-li, ZHAO Yuan-yuan, XIAO Xue- li, XU Rong- rong, BAO Xue- mei. Study on the preparation technology of hemin from enzymolysis yak blood by papain[J]. Science and Technology of Food Industry, 2016, (03): 206-210. doi: 10.13386/j.issn1002-0306.2016.03.035

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Study on the preparation technology of hemin from enzymolysis yak blood by papain

doi: 10.13386/j.issn1002-0306.2016.03.035

College of Food Science and Technology,Gansu Agriculture University

Received Date: 2015-07-13

Abstract

Abstract

Preparation hemin with high content hemoglobin in yak blood.With fresh yak blood as the material to get the hemoglobin through pre- treatment,then through enzymolysis to get the hemin.On the basement of single factor test,using combination Box-Benhnken center rotation experiment and response surface analysis,the best extraction process of hemin from hemoglobin by enzymolysis as follows: 55 ℃ for enzymolysis temperature,94 min and p H8.0for enzymolysis,enzyme concentration was 0.08 g. After optimization,the yield of hemin reached 6.47 μg / m L. The enzymolysis yak blood by papain extract hemin is a useful method.
- yak blood,
- papain,
- hemin,
- response surface analysis

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References(23)

References

[1]	于海燕,罗永康.对牦牛血理化性质的研究[J].肉类研究,2007(6):33-36.
[2]	朱森阳,汪国和.王志鹏.畜禽血液资源的开发和利用进展[J].饲料与营养,2007(1):67-68.
[3]	汪丰云.血液中的科学[J].畜牧兽医,2006(12):59-63.
[4]	袁曦,洪清,吕方生,等.氯化血红素胶囊的研制及临床疗效[J].中国药房,1996,7(1):17-18.
[5]	宋照军,张延生,刘玺,等.猪血中血红素提取纯化新技术研究[J].肉类工业,2004,(8):23-25.
[6]	王君,康平利.超声波法提取氯化血红素的研究[J].药物生物技术,2004,11(4):260-263.
[7]	In Man Jin,Hee J eong Chae,Oh Nam Soon.Process development for heme enriched peptide by enzymatic hydrolysis of hemo globin[J].Bioresource Technology,2002,84(1):63-68.
[8]	吴保承,沈国强.酶法提取猪血中血红素的工艺研究[J].化学与生物工程,2009,(26):61-63.
[9]	张亚娟.从猪血中酶法制取血红素的研究[D].无锡:江南大学,2007.
[10]	姜红,施用晖.木瓜蛋白酶水解马鹿茸血制备免疫活性肽的研究[J].氨基酸和生物资源,2008,30(1):72-76.
[11]	孙海梅.我国加入WTO后青海牦牛业发展对策[J].畜牧与兽医,2002,(34):8-12.
[12]	吴保承,沈国强.酶法提取猪血中血红素的工艺研究[J].化学与生物工程,2009,(26):61-63.
[13]	张玉斌,郭兆斌.牛血资源综合开发利用研究进展[J].肉类研究,2011,(25):30-34.
[14]	赵剑龙.响应面法优化马血中氯化血红素提取工艺的研究[J].加工与研究,2013,13(5):38-42.
[15]	杨锡洪,夏文水.酶法水解血红蛋白制备亚铁血红素肽[J].食品与机械,2005,21(3):l4-l6.
[16]	仇凯,陈志南.木瓜蛋白酶制备抗人肝癌单抗F(ab')2及Fab片段[J].第四军医大学学报,1995,16(6):414-417.
[17]	大连轻工业学院.食品分析[M].北京:中国轻工业出版社,1995:221-223.
[18]	王光亚.保健食品功效成分检测方法[M].北京:中国轻工业出版社,2002.
[19]	张亚娟.从猪血中酶法制取血红素的研究[D].无锡:江南大学,2007,(16):46-49.
[20]	Branen A L,Davidson P M,Salminen S,et al.Antioxidants in:Food additives.Marcel.Dekker.Inc.1990,pp,139-193.
[21]	叶林奇.组氨酸在蛋白质结构功能中的作用[J].涪陵师专学报,2000,16(4):21-24.
[22]	Fiedler T J,Davey C A,Fenna R E.X-ray crystal structure and characterization of halide-binding sites of human myeloperxidase at 1.8 angstrom resolution[J].J Biol Chem.2000,(275):11964-11971.
[23]	赵剑龙.响应面法优化马血中氯化血红素提取工艺的研究[J].加工与研究,2013,13(5):38-42.