Optimization of fermentation technology for production of fructosyltransferase with Aspergillus oryzae using CCD response surface methods

WANG Peng; JIANG Bo; WANG Sheng- hai; FAN Sen; LIN Xiao- shan; ZHANG Yi

doi:10.13386/j.issn1002-0306.2016.03.043

Article Contents

Article Navigation > Science and Technology of Food Industry > 2016 > (03): 246-250

WANG Peng, JIANG Bo, WANG Sheng- hai, FAN Sen, LIN Xiao- shan, ZHANG Yi. Optimization of fermentation technology for production of fructosyltransferase with Aspergillus oryzae using CCD response surface methods[J]. Science and Technology of Food Industry, 2016, (03): 246-250. doi: 10.13386/j.issn1002-0306.2016.03.043

Citation:

WANG Peng, JIANG Bo, WANG Sheng- hai, FAN Sen, LIN Xiao- shan, ZHANG Yi. Optimization of fermentation technology for production of fructosyltransferase with Aspergillus oryzae using CCD response surface methods[J]. Science and Technology of Food Industry, 2016, (03): 246-250. doi: 10.13386/j.issn1002-0306.2016.03.043

Citation:

WANG Peng, JIANG Bo, WANG Sheng- hai, FAN Sen, LIN Xiao- shan, ZHANG Yi. Optimization of fermentation technology for production of fructosyltransferase with Aspergillus oryzae using CCD response surface methods[J]. Science and Technology of Food Industry, 2016, (03): 246-250. doi: 10.13386/j.issn1002-0306.2016.03.043

PDF( 897 KB)

Optimization of fermentation technology for production of fructosyltransferase with Aspergillus oryzae using CCD response surface methods

doi: 10.13386/j.issn1002-0306.2016.03.043

School of Bioscience and Bioengineering,South China University of Technology

Received Date: 2015-06-08

Abstract

Abstract

In order to produce fructosyltransferase in large scale,it is necessary to study and optimize the process conditions of Aspergillus oryzae strains fermentation. Using sucrose,soybean meal powder and corn flour as the basic medium for fructosyltransferase synthesis in the cells,three significant factors that affect the fermentation course were selected through the Plackett- Burman optimization experiment.With analysis of the central composite design( CCD) response surface methods,the fermentation process has been further optimized and the results showed that: there were interactions between the concentration of sucrose,soybean meal powder and the rotation speed.The interaction between the concentration of soybean meal powder and the rotation speed was mainly significant.The best technical parameters were obtained as followed: the concentration of sucrose was 5.95%,the concentration of soybean meal powder was 1.60%,and the rotation speed was 165 r / min. Under the optimal condition of fermentation,the enzyme activity of fructosyltransferase produced by fusion strain Aspergillus oryzae RIII-7 reached 870.21 U / g.It is close to the predictive value of 895.68 U / g,with an error of 0.028.
- Aspergillus oryzae,
- fructosyltransferase,
- Plackett-Burman optimization,
- RSM,
- fermentation

FullText(HTML)

References(14)

References

[1]	杨正茂,秦克亮,赵玉秀,等.β-果糖基转移酶和β-呋喃果糖苷酶的分离纯化[J].中国医药工业杂志,2002,33(5):219-223.
[2]	覃益民,唐江涛,苏加坤,等.米曲霉GX0011β-果糖基转移酶的性质研究[J].食品工业科技,2007,28(5):123-126.
[3]	魏远安,姚评佳,谢庆武,等.游离及固定化果糖基转移酶部分酶学性质的比较研究[J].中国生物化学与分子生物学报,2001,17(4):501-505.
[4]	Sprenger N,Bortlik K,Brandt A,et al.Purification,cloning,and functional expressionof sucrose:fructan-fructosyltransferase,a key enzyme of fructan synthesis in barley[J].Proceedings of the National Academy of Sciences,1995,92(25):11652-11656.
[5]	Hisano H,Kanazawa A,Kawakami A,et al.Transgenic perennial ryegrass plantsexpressing wheat fructo-syltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing[J].Plant Science,2004,167(4):861-868.
[6]	Shiomi N.Properties of fructosyltransferases involved in the synthesis of fructan in liliaceous plant[J].Journal of Plant Physiology,1989,134(2):151-155.
[7]	Mohd Anis Ganaie,Uma Shanker Gupta,Naveen Kango,et al.Screening of biocatalysts for transformation of sucrose to fructooligosaccharides[J].Journal of Molecular Catalysis.B,Enzymatic,2013,97:12-17.
[8]	Avineri-Shapiro S,Hestrin S.The mechanism of polysaccharide production from sucrose.2.[J].Biochemical Journal,1945,39(2):167-172.
[9]	唐江涛,覃益民,杨梅,等.利用重组葡萄糖脱氢酶提高低聚果糖纯度[J].食品研究与开发,2007,28(12):63-67.
[10]	Mohd Anis Ganaie,Hemant Kumar Rawat,Owais Ahmad Wani,et al.Immobilization of fructosyltransfer-ase by chitosan and alginate for efficient production of fructooligosaccharides[J].Process Biochemistry,2014,49(5):840-844.
[11]	Mohd Anis Ganaie,Uma Shanker Gupta.Recycling of cell culture and efficient release of intracellular fructosyltransferase by ultrasonication for the production of fructooligosaccharides[J].Carbohydrate Polymers,2014,110:253-258.
[12]	何小妮,蒋波,王玉海,等.非对称灭活双亲原生质体融合法选育产果糖基转移酶的米曲霉新菌株的研究[J].现代食品科技,2013,29(5):993-997.
[13]	何小妮,陈子健,蒋波,等.高产果糖基转移酶米曲霉菌株的选育[J].食品工业科技,2013,34(22):135-140.
[14]	Marcelaedová,Viera Illeová,Monika Antoová,et al.Production of fructosyltransferase in mechanically stirred and airlift bioreactors[J].Chemical Papers,2014,68(12):1639-1648.