Determination of capsaicinoids and piquancy characteristic of Zhejiang dishes

HE Shuai; XIE Ding-yuan

doi:10.13386/j.issn1002-0306.2016.04.007

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Article Navigation > Science and Technology of Food Industry > 2016 > (04): 80-86

HE Shuai, XIE Ding-yuan. Determination of capsaicinoids and piquancy characteristic of Zhejiang dishes[J]. Science and Technology of Food Industry, 2016, (04): 80-86. doi: 10.13386/j.issn1002-0306.2016.04.007

Citation:

HE Shuai, XIE Ding-yuan. Determination of capsaicinoids and piquancy characteristic of Zhejiang dishes[J]. Science and Technology of Food Industry, 2016, (04): 80-86. doi: 10.13386/j.issn1002-0306.2016.04.007

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Determination of capsaicinoids and piquancy characteristic of Zhejiang dishes

doi: 10.13386/j.issn1002-0306.2016.04.007

College of Food Science,Technology of Huazhong Agricultural University

Received Date: 2015-06-30

Abstract

Abstract

Objective:The aim of this study was to find a efficient and faster way for the determination of main spicy ingredients in dishes through optimizing the detection chromatographic condition according to GB/T21266-2007. Taking a part of Zhejiang dishes that collected from Shaoxing,Hangzhou,Ningbo and Wenzhou as the experimental subject,detecting spicy ingredients in each dish,then analyzed the piquancy characteristics of Zhejiang dishes. Method:Capsaicin and dihydrocapsaicin were extracted by ultrasonic with methanol-tetrahydrofuran( 1 ∶ 1) as solvent, high performance liquid chromatography( HPLC) was used to analyze capsaicinoid compounds. Result:The mobile phase was methanol:water(70∶30) with the flow rate was 0.8 m L/min and the UV detection wavelength was set at 280 nm. The regression equation of capsaicin was Y =6636.7X +1221 and correlation coefficient R2=0.9999. The regression equation of dihydrocapsaicin was Y=6338.9X-3758 and correlation coefficient R2=0.9999. The proportion of the use chili in Zhejiang 100 dishes was 13%,the spicy degree of whole dishes was 0.143. Conclusion:This method was accurate,speedy and reproducible.Pungency level in Zhejiang dishes was low.
- capsaicinoid,
- Zhejiang dishes,
- extraction,
- HPLC,
- pungency degree

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References(20)

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