Issue 04
Turn off MathJax
Article Contents
ZENG Li-ping, WANG Xin-shi, WU Su-rui, FAN Jian, ZHAO Tian-rui. Purification and antioxidant activity of polysaccharide from Boletus aereus in Yunnan[J]. Science and Technology of Food Industry, 2016, (04): 151-154. doi: 10.13386/j.issn1002-0306.2016.04.021
Citation: ZENG Li-ping, WANG Xin-shi, WU Su-rui, FAN Jian, ZHAO Tian-rui. Purification and antioxidant activity of polysaccharide from Boletus aereus in Yunnan[J]. Science and Technology of Food Industry, 2016, (04): 151-154. doi: 10.13386/j.issn1002-0306.2016.04.021

Purification and antioxidant activity of polysaccharide from Boletus aereus in Yunnan

doi: 10.13386/j.issn1002-0306.2016.04.021
  • Received Date: 2015-07-27
  • The purification and activity analysis of Boletus aereus polysaccharides can provide a theoretical basis for the further application and popularization of B. aereus. In the present work,crude polysaccharides of Bronze Boletus was extracted by hot water and then successively purified with DEAE-52 ion exchange column and Sephadex G-100 gel permeadun column. Two polysaccharides,named PB-1A and PB-2A,were obtained after purification. The purity of obtained polysaccharide was determined by specific rotation method and UV spectral scanning. The total reducing power,hydroxyl free radical and DPPH free radical scavenging activity of crude polysaccharides,PB-1A and PB-2A were determined by in vitro antioxidant evaluation system. The results demonstrated that the total reducing power,hydroxyl free radical and DPPH free radical scavenging activity of B. aereus polysaccharides before and after purification showed a dose- dependent manner, with activities being PB-2A>PB-1A>crude polysaccharides of B. aereus.
  • loading
  • [1]
    张亚光.中国常见食用菌图鉴[M].昆明:云南科技出版社,1999:65.
    [2]
    黄俊丽,张慜.高温蒸汽瞬时漂烫对黑牛肝菌酶活和品质影响的研究[J].食品与生物技术学报,2010,29(5):653-659.
    [3]
    赵云霞,陶眀煊,郭永月,等.黑牛肝菌多糖对酒精性损伤小鼠心脏及脾脏抗氧化作用的研究[J].南京师大学报:自然科学版,2014,37(1):133-136.
    [4]
    杨立红,刘林德,钟旭生,等.野生牛肝菌多糖的分离鉴定及其抗氧化性研究[J].食品科学,2008,29(8):335-338.
    [5]
    黄俊丽,张慜.松茸、黑牛肝菌、双孢白蘑菇提取物体外抗氧化性实验研究[J].食品与生物技术学报,2011,30(3):342-347.
    [6]
    岳万松,熊勇,陈毅坚.云南牛肝菌的内生真菌分离、鉴定和ITS序列特征研究[J].食品工业科技,2014,35(19):172-176.
    [7]
    王心诗,邰丽梅,吴素蕊,等.响应面法优化水提铜色牛肝菌多糖工艺[J].中国食用菌,2015,34(1):60-64.
    [8]
    巴媛媛,王莹,朴美子.苯酚-硫酸法测定瓦尼木层孔菌菌丝体多糖含量的条件优化[J].食品工业科技,2011,32(5):389-391.
    [9]
    闫景坤,李琳,吴建勇.人工培养冬虫夏草胞外多糖的分离纯化研究[J].现代食品科技,2010,26(4):366-369.
    [10]
    陈小娥,方旭波,钟秋琴.蛎菜酸性多糖的分离纯化和降血糖活性研究[J].中国食品学报,2008,8(5):75-79.
    [11]
    Smirnoff N,Cumbes Q.Hydroxyl radical scavenging activity of compatible solutes[J].Phytochemistry,1989,28(4):1057-1060.
    [12]
    李波,徐贵华,芦菲,等.七种云南产食用菌的抗氧化活性研究[J].食用菌,2010,32(2):66-67.
    [13]
    Oyaizu M.Studies on products of browning reactions:Antioxidative activities of products ofbrowning reaction prepared from glucosamine[J].Japanese Journal of Nutrition,1986,44:307-315.
    [14]
    朱月,李彩霞,毕晓丹.猪苓多糖分级纯化及对羟自由基清除作用的研究[J].安徽农业科学,2011,39(30):18553-18555.
    [15]
    王和才,胡秋辉.DPPH法测定紫红薯提取物清除自由基的能力[J].食品研究与开发,2010,31(1):132-135.
    [16]
    侯秀娟,沈勇根,徐明生,等.化橘红多糖的提取纯化及抗氧化活性研究[J].中国酿造,2012,31(9):135-138.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (50) PDF downloads(274) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return