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SHAO Shuang-shuang, HE Liang, WEI Chao-yang, LI Wei-qi. Chain conformation and antioxidant activity of a novel intracellular polysaccharide HSIPS2 from Hirsutella sinensis[J]. Science and Technology of Food Industry, 2016, (04): 200-204. doi: 10.13386/j.issn1002-0306.2016.04.032
Citation: SHAO Shuang-shuang, HE Liang, WEI Chao-yang, LI Wei-qi. Chain conformation and antioxidant activity of a novel intracellular polysaccharide HSIPS2 from Hirsutella sinensis[J]. Science and Technology of Food Industry, 2016, (04): 200-204. doi: 10.13386/j.issn1002-0306.2016.04.032

Chain conformation and antioxidant activity of a novel intracellular polysaccharide HSIPS2 from Hirsutella sinensis

doi: 10.13386/j.issn1002-0306.2016.04.032
  • Received Date: 2015-08-24
  • A novel intracellular polysaccharide,named HSIPS2,from Hirsutella sinensis was obtained by hot water extraction and alcohol precipitation,and then purified using DEAE-Sepharose Fast Flow chromatography and Sephacryl S-100 gel column chromatography. Its chain conformation and antioxidant activity were determined.The result showed that HSIPS2 was composed of glucose,galactose,mannose,and ribose with a molar ratio of48.52 ∶6.58 ∶5.17 ∶1.0. It was proved to be a novel lower dispersion polysaccharide with uniform molecular of1.463×10~4g/mol,and the purity was 99.82%. In 0.1 mol/L Na NO_3 aqueous solution,HSIPS2 existed as flexible chain conformation. The parameters of Rh,ML,q and d were 2.3 nm,334.05 nm~/(-1),0.70 nm,and 0.69 nm respectively.Moreover,the IC_(50) of its hydroxyl radical scavenging activity was identified to be 0.749 mg/m L and the value of ORAC was calculated to be 872.80 μmol TE/g. Hence,it was undoubtedly demonstrated that HSIPS2 exhibited potent antioxidant activity.
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  • [1]
    赵锦,李泰辉.冬虫夏草无性型的分子鉴别[J].中山大学学报:自然科学版,1999,38(1):121-123.
    [2]
    李增智,黄勃,李春如,等.确证冬虫夏草无性型的分子生物学证据1.中国被毛孢与冬虫夏草之间的关系[J].菌物系统,2000,19(1):60-64.
    [3]
    蒋毅,姚一建.冬虫夏草无性型研究概况[J].菌物系统,2003,22(1):161-176.
    [4]
    谢明勇,聂少平.天然产物活性多糖结构与功能研究进展[J].中国食品学报,2010,10(2):1-11.
    [5]
    Yan J K,Wang W Q,Wu J Y.Recent advances in Cordyceps sinensis polysaccharides:Mycelial fermentation,isolation,structure,and bioactivities:A review[J].Journal of Functional Foods,2014,6:33-47.
    [6]
    刘金花,李富奎,贾得儒,等.中国被毛孢发酵虫草菌丝体多糖的提取,纯化及其理化性质[J].食品与发酵工业,2014,40(3):222-226.
    [7]
    丁婷,邵颖,樊美珍.中国被毛孢发酵液中一种镇静催眠活性物质的分离纯化和结构鉴定[J].菌物学报,2008,27(6):956-963.
    [8]
    He L,Cheng J W,Wang Y B,et al.Statistics-based optimization of extracellular polysaccharide production from Hirsutella sinensis using a fermentation process and in vitro immunomodulatory activity[J].Food Science and Biotechnology,2014,23(2):451-457.
    [9]
    王蕾,于荣敏,张辉,等.人工培养蛹虫草多糖的分离纯化及其结构的初步研究[J].中国生化药物杂志,2003,24(1):23-25.
    [10]
    Dai J,Wu Y,Chen S,et al.Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone[J].Carbohydrate Polymers,2010,82(3):629-635.
    [11]
    Wu D T,Meng L Z,Wang L Y,et al.Chain conformation and immunomodulatory activity of a hyperbranched polysaccharide from Cordyceps sinensis[J].Carbohydrate Polymers,2014,110:405-414.
    [12]
    Rau U,Müller R J,Cordes K,et al.Process and molecular data of branched 1,3-β-D-glucans in comparison with Xanthan[J].Bioprocess Engineering,1990,5(2):89-93.
    [13]
    Mao G,Zou Y,Feng W,et al.Extraction,preliminary characterization and antioxidant activity of Se-enriched Maitake polysaccharide[J].Carbohydrade Polymer,2014,101(30):213-219.
    [14]
    Hua Y,Yang B,Tang J,et al.Structural analysis of watersoluble polysaccharides in the fruiting body of Dictyophora indusiata and their in vivo antioxidant activities[J].Carbohydrate Polymers,2012,87(1):343-347.
    [15]
    刘金花,李富奎,贾得儒,等.中国被毛孢发酵虫草菌丝体多糖的提取,纯化及其理化性质[J].食品与发酵工业,2014,40(3):222-226.
    [16]
    季志超.基于临界胶束浓度控制的“假高稀法”高效合成环状嵌段共聚物及其性能研究[D].苏州:苏州大学,2014.
    [17]
    伊芳,郭丽,杜先锋.SEC-MALLS-RI体系测定魔芋胶分子结构特性的研究[J].安徽农业科学,2011,39(36):22585-22588.
    [18]
    Chen C,Wu W,Xu X,et al.Chain conformation and antitumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici[J].Carbohydrate Polymers,2014,105:308-316.
    [19]
    Wei C,Li W,Shao S,et al.Structure and chain conformation of a neutral intracellular heteropolysaccharide from mycelium of Paecilomyces cicadae[J].Carbohydrate Polymers,2016,136:728-737.
    [20]
    许淑琴.黑木耳刚性链葡聚糖结构,链构象转变及自组装行为[D].武汉:武汉大学,2013.
    [21]
    Yang L,Zhang L M.Chemical structural and chain conformational characterization of some bioactive polysaccharides isolated from natural sources[J].Carbohydrate Polymers,2009,76(3):349-361.
    [22]
    邵丽.产胞外多糖乳杆菌的筛选及其多糖的分离,结构和生物活性研究[D].无锡:江南大学,2015.
    [23]
    Liu J H,Wang Z J,Wang Y,et al.Structural Elucidation and Antioxidant Activity of a Polysaccharide from Mycelia Fermentation of Hirsutella sinensis Isolated from Ophiocordyceps sinensis[J].Journal of Bioprocessing&Biotechniques,2014,4(7):1.
    [24]
    Wojcikowski K,Stevenson L,Leach D,et al.Antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system:a comparison using a sequential three-solvent extraction process[J].The Journal of Alternative and Complementary Medicine,2007,13(1):103-110.
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