Synthesis of recombinant gene for Bacillus subtilis bacteriocin subtilosin A and its expression in Pichia pastoris

CHEN Bai-xiong; HUANG Jia-jun; LIN Jun-fang; GUO Li-qiong; CHEN Jun-fei; LIU Ying-li

doi:10.13386/j.issn1002-0306.2016.04.037

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Article Navigation > Science and Technology of Food Industry > 2016 > (04): 228-231

CHEN Bai-xiong, HUANG Jia-jun, LIN Jun-fang, GUO Li-qiong, CHEN Jun-fei, LIU Ying-li. Synthesis of recombinant gene for Bacillus subtilis bacteriocin subtilosin A and its expression in Pichia pastoris[J]. Science and Technology of Food Industry, 2016, (04): 228-231. doi: 10.13386/j.issn1002-0306.2016.04.037

Citation:

CHEN Bai-xiong, HUANG Jia-jun, LIN Jun-fang, GUO Li-qiong, CHEN Jun-fei, LIU Ying-li. Synthesis of recombinant gene for Bacillus subtilis bacteriocin subtilosin A and its expression in Pichia pastoris[J]. Science and Technology of Food Industry, 2016, (04): 228-231. doi: 10.13386/j.issn1002-0306.2016.04.037

Citation:

CHEN Bai-xiong, HUANG Jia-jun, LIN Jun-fang, GUO Li-qiong, CHEN Jun-fei, LIU Ying-li. Synthesis of recombinant gene for Bacillus subtilis bacteriocin subtilosin A and its expression in Pichia pastoris[J]. Science and Technology of Food Industry, 2016, (04): 228-231. doi: 10.13386/j.issn1002-0306.2016.04.037

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Synthesis of recombinant gene for Bacillus subtilis bacteriocin subtilosin A and its expression in Pichia pastoris

doi: 10.13386/j.issn1002-0306.2016.04.037

Department of Bioengineering,College of Food Science,South China Agricultural University,Institute of Food Biotechnology,South China Agricultural University
The School of Chemistry and Pharmaceutical Engineering,Qi-Lu University of Technology
Beijing Laboratory for Food Quality and Safety,Beijing Technology and Business University

Received Date: 2015-06-01

Abstract

Abstract

In this study,the recombinant gene for subtilosin A(rsbo A) was synthesis for the sake of the expression for this bacteriocin in host cell P. pastoris. Both the results of colony PCR of positive Escherichia coli recombinants and DNA sequencing indicated that recombinant plasmid p PIC9K- sbo was constructed successfully. In addition,the results of colony PCR of positive P. pastoris recombinants indicated that recombinant Pichia GS115- p PIC9K- sbo was constructed successfully. Protein electrophoresis analysis of positive P. pastoris recombinants displayed that the recombinant gene rsbo A was secretory expressed in P. pastoris GS115.Analysis of antimicrobial activity showed that the fermentation broth of this recombinant yeast possess antibacterial activities against P. aeruginosa. The result of this work built a new strategy of bacteriocins,a small molecule peptide,in heterologous expression. The study laid a foundation for industrialized production of Bacillus subtilis antibiotic peptide subtilosin A.
- subtilosin A,
- genetic recombinant,
- Pichia pastoris,
- antibacterial activity

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References(12)

References

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