Issue 04
Turn off MathJax
Article Contents
CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, TAO Ran, LI Wen-jun. Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity[J]. Science and Technology of Food Industry, 2016, (04): 254-258. doi: 10.13386/j.issn1002-0306.2016.04.043
Citation: CHEN Hong-xia, WANG Cheng-zhang, YE Jian-zhong, ZHOU Hao, TAO Ran, LI Wen-jun. Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity[J]. Science and Technology of Food Industry, 2016, (04): 254-258. doi: 10.13386/j.issn1002-0306.2016.04.043

Extraction optimization of crude polysaccharides from the seed cakes of Rhus succedanea L and its antioxidant activity

doi: 10.13386/j.issn1002-0306.2016.04.043
  • Received Date: 2015-08-24
  • Single factor and orthogonal methodology were used to optimize the extraction conditions of crude polysaccharides from the seed cakes of Rhus succedanea L,then the antioxidant(scavenging hydroxyl and ABTS radicals) of the crude polysaccharides from the seed cakes of Rhus succedanea L were possessed. The optimum extraction conditions were as follows :extraction time 2.5 h,ratio of material to water 1 ∶20(g∶m L),extraction temperature 80 ℃,and extraction times 2. Under the optimized conditions,an experimental yield was 1. 561 %. The IC_(50) values scavenging hydroxyl and ABTS~+radicals of the crude polysaccharides were8.515 mg·m L~(-1) and 7.03 mg·m L~(-1),respectively. The hydroxyl radical scavenging ability of polysaccharides reached 61.5% at 25 mg·m L~(-1). The ABTS+radicals scavenging ability of polysaccharides reached 58.4% at10 mg·m L~(-1). The seed cakes polysaccharides displayed significant antioxidant.
  • loading
  • [1]
    董艳鹤,王成章,宫坤,等.漆树资源的化学成分及其综合利用研究进展[J].林产化学与工业,2009,29(B10):225-232.
    [2]
    Zong A Z,Cao H Z,Wang F S.Anticancer polysaccharides from natural resources:A review of recent research[J].Carbohydrate Polymers,2012,90(4):1395-1410.
    [3]
    Cho E J,Hwang H J,Kim S W,et al.Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice[J].Applied Microbiology and Biotechnology,2007,75(6):1257-1265.
    [4]
    Suvakanta D,Narsimha M P,Pulak D,et al.Optimization and characterization of purified polysaccharide from Musa sapientum L.as a pharmaceutical excipient[J].Food Chemistry,2014,149:76-83.
    [5]
    Abou Zeid A H,Aboutabl E A,Sleem A A,et al.Water soluble polysaccharides extracted from Pterocladia capillacea and Dictyopteris membranacea and their biological activities[J].Carbohydrate Polymers,2014,113:62-66.
    [6]
    Facchini J M,Alves E P,Aguilera C,et al.Antitumor activity of Pleurotus ostreatus polysaccharide fractions on Ehrlich tumor and Sarcoma 180[J].International Journal of Biological Macromolecules,2014,68:72-77.
    [7]
    Pakrokh Ghavi P.The extraction process optimization of antioxidant polysaccharides from Marshmallow(Althaea officinalis L.)roots[J].International Journal of Biological Macromolecules,2015,75:51-57.
    [8]
    董艳鹤,王成章,叶建中,等.漆蜡的提取工艺及其化学成分[J].北京林业大学学报,2010,(4):256-260.
    [9]
    董艳鹤,王成章,陈虹霞,等.漆蜡的吸附脱色工艺研究[J].林产化学与工业,2011,31(2):91-95.
    [10]
    陈虹霞,王成章,叶建中,等.漆蜡脂肪酸甲酯磺酸盐的制备及其性能研究[J].中国油脂,2014,39(5):37-40.
    [11]
    Chung S K,Osawa T,Kawakishi S.Hydroxyl radical scavenging effects of species and scavengers from brown mustard(Brassia nigra)[J].Bioscience Biotehnology and Biochemistry,1997,61:118-123.
    [12]
    Re R,Pellegrini N,Proteggente A,et al.Antioxidant activity applying and improved ABTS radical cation ecolorization assay[J].Free Radic Biol Med,1999,26(9):1231-1237.
    [13]
    Chance B,Sies H,Boveris A.Hydroperoxide metabolism in mammalian organs[J].Physiological Reviews,1979,59:527-605.
    [14]
    Bohn J A,Be Miller J N.(1→3)-β-d-Glucan as biological response modifiers:A review of structure-functional activity relationships[J].Carbohydrate Polymers,1995,28:3-14.
    [15]
    Miller N J,Rice-Evans C A,Davies M J,et al.A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates[J].Clin Sci,1993,84:407-412.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (101) PDF downloads(179) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return