Response surface optimizes the compound extraction process of sulforaphane from broccoli

HU Cui-zhen; LI Sheng; MA Shao-ying; CAO Bao-chen; TANG Bin; ZHAO Sheng-qin; SHI Zhen-zhen; SU Li-rong; ZHOU Wen-zheng

doi:10.13386/j.issn1002-0306.2016.04.046

Article Contents

Article Navigation > Science and Technology of Food Industry > 2016 > (04): 271-277

HU Cui-zhen, LI Sheng, MA Shao-ying, CAO Bao-chen, TANG Bin, ZHAO Sheng-qin, SHI Zhen-zhen, SU Li-rong, ZHOU Wen-zheng. Response surface optimizes the compound extraction process of sulforaphane from broccoli[J]. Science and Technology of Food Industry, 2016, (04): 271-277. doi: 10.13386/j.issn1002-0306.2016.04.046

Citation:

HU Cui-zhen, LI Sheng, MA Shao-ying, CAO Bao-chen, TANG Bin, ZHAO Sheng-qin, SHI Zhen-zhen, SU Li-rong, ZHOU Wen-zheng. Response surface optimizes the compound extraction process of sulforaphane from broccoli[J]. Science and Technology of Food Industry, 2016, (04): 271-277. doi: 10.13386/j.issn1002-0306.2016.04.046

Citation:

HU Cui-zhen, LI Sheng, MA Shao-ying, CAO Bao-chen, TANG Bin, ZHAO Sheng-qin, SHI Zhen-zhen, SU Li-rong, ZHOU Wen-zheng. Response surface optimizes the compound extraction process of sulforaphane from broccoli[J]. Science and Technology of Food Industry, 2016, (04): 271-277. doi: 10.13386/j.issn1002-0306.2016.04.046

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Response surface optimizes the compound extraction process of sulforaphane from broccoli

doi: 10.13386/j.issn1002-0306.2016.04.046

Gansu Provincial Key Lab of Arid land Crop Science,College of Life Science and Technology,Gansu Agricultural University
Lanzhou Huitong Bio-Technology Co.,Ltd.
Gansu Shunyi Bio-Technology Co.,Ltd.

Received Date: 2015-06-15

Abstract

Abstract

The green young stems and buds of broccoli were used as experimental material,the enzymolysis condition of sulforaphane extraction from broccoli was optimized by response surface method. According to central composite experiment design principle and using response surface analysis of four factors and three levels,four additives including ascorbic acid,EDTA,Mg SO_4,Ca Cl_2 were chosen on the basis of the single factor to determine the impact of various factors on the extraction rate of sulforaphane. The optimum extraction conditions were as follows:ascorbic acid 4.659 mg/g,EDTA 6 mmol/L,Mg SO_4 8.5 mmol/L,Ca Cl_2 4.0 mmol/L.The theoretical maximum extraction rate of sulforaphane was 1858.20 μg/g,and the value reached 1813.26 μg/g in the experiment,the relative error was 2.48%,which indicated that the extraction process parameter above was feasible in sulforaphane extraction from broccoli.
- sulforaphane,
- enzymolysis,
- additive,
- single factor,
- response surface

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References(20)

References

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