Optimization of Ginkgo protein extraction by response surface methology

ZHANG Xiu-yun; LI Dan-dan; MA Yan-fang

doi:10.13386/j.issn1002-0306.2016.04.051

Article Contents

Article Navigation > Science and Technology of Food Industry > 2016 > (04): 299-302

ZHANG Xiu-yun, LI Dan-dan, MA Yan-fang. Optimization of Ginkgo protein extraction by response surface methology[J]. Science and Technology of Food Industry, 2016, (04): 299-302. doi: 10.13386/j.issn1002-0306.2016.04.051

Citation:

ZHANG Xiu-yun, LI Dan-dan, MA Yan-fang. Optimization of Ginkgo protein extraction by response surface methology[J]. Science and Technology of Food Industry, 2016, (04): 299-302. doi: 10.13386/j.issn1002-0306.2016.04.051

Citation:

PDF( 871 KB)

Optimization of Ginkgo protein extraction by response surface methology

doi: 10.13386/j.issn1002-0306.2016.04.051

Department of Information Engineering,Laiwu Vocational and Technical College
Heze Food and Drug Administration
College of Life Sciences,Shandong Agricultural University

Received Date: 2015-07-24

Abstract

Abstract

The response surface analysis method was used to study the Ginkgo protein extraction process on the basis of single factor experiment with Ginkgo for materials,the effect of Tris-HCl concentration,p H,solidliquid rate,extraction time on the protein extraction yield was inspected. The research results showed that the optimal conditions for ginkgo protein extraction:0.16 mol/L,p H8.7,1∶26(g/m L),and 5 h. Under this condition,the yield of Ginkgo protein was 82.76%,compared to the theoretical value,the relative error of 0.30%. Optimized regression equation derived some practical significance.
- Ginkgo protein,
- extraction,
- response surface method

FullText(HTML)

References(18)

References

[1]	黄文,谢笔钧.白果的研究和开发利用[J].湖北林业科技,2002(3):41-42.
[2]	李思斯,江波,张涛,等.银杏外种皮总黄酮的提取及其抗氧化活性研究[J].食品工业科技,2011,32(8):291-294.
[3]	Liu H J,Wang X L,Zhang L,et al.Inhibitions of vascular endothelial growth factor expression and foamation by EGb 761,a special extract of Ginkgo biloba,in oxidatively modified lowdensity lipoprotein-induced human THP-1 monocytes cells[J].Phytomedicine,2009,16(2):138-145.
[4]	黄文,谢笔钧,姚平,等.白果活性蛋白的抗生物氧化作用[J].营养学报,2002,24(2):192-194.
[5]	黄文,谢笔钧,王益,等.白果蛋白质的分离、纯化、理化特性及其抗氧化活性研究[J].中国农业科学,2004,37(10):1537-1543.
[6]	Huang Wen,Deng Qianchun,Xie bijun,et al.Purification and characterization of an antioxidant protein from Ginkgo biloba seeds[J].Food Research International,2010,43(1):86-94.
[7]	李莹莹,吴彩娥,杨剑婷,等.白果蛋白质提取及SDS-PAGE分析[J].食品科学,2010,31(22):36-40.
[8]	孙小斐,乔玉辉.白果蛋白的提取分离及其等电点的测定[J].食品工业,2012(9):156-158.
[9]	王艾平,周丽明,考马斯亮蓝法测定茶籽多糖中蛋白质含量条件的优化[J].河南农业科学,2014,43(3):150-153.
[10]	罗群.考马斯亮蓝法快速测定菜籽粕中可溶性蛋白质的含量[J].成都大学学报自然版,2014,33(2):125-127.
[11]	许亚平,林俊兵.蛋白质提纯研究进展[J].天津化工,2006,20(4):9-11.
[12]	张相年,赵树进,李超.蛋白分离技术的应用和进展[J].中国药业,2006,15(2):72-73.
[13]	李顺灵,严有兵,李向珍.食用菜籽蛋白的提取分离及其应用研究[J].食品工业科技,2007,14(3):12-14.
[14]	陈源,杨道富,范丽华,等.响应面法优化微波提取茂谷桔橙皮总黄酮工艺[J].中国食品学报,2013,13(4):80-85.
[15]	戴喜末,熊子文,罗丽萍.响应面法优化野艾蒿多糖的超声波提取及其抗氧化性研究[J].食品科学,2011,32(8):93-97.
[16]	王岸娜,孙玉丹,李龙安,等.响应面法优化猕猴桃糖蛋白提取工艺研究[J].河南农业科学,2012,41(8):121-127.
[17]	王淑霞,李爱梅,张俊杰,等.响应面分析法优化龙眼核中多酚物质提取工艺[J].食品科学,2011,32(10):35-39.
[18]	袁建,李大川,石嘉怿,等.响应面法优化麦麸蛋白质和膳食纤维的提取工艺[J].食品科学,2011,32(10):25-30.