Issue 04
Turn off MathJax
Article Contents
ZHENG Shuang, LI Xin-hua, YANG Qiang, SUN Bu-yun. Optimization study on purification of polysaccharides in polygonatum odoratum[J]. Science and Technology of Food Industry, 2016, (04): 322-325. doi: 10.13386/j.issn1002-0306.2016.04.056
Citation: ZHENG Shuang, LI Xin-hua, YANG Qiang, SUN Bu-yun. Optimization study on purification of polysaccharides in polygonatum odoratum[J]. Science and Technology of Food Industry, 2016, (04): 322-325. doi: 10.13386/j.issn1002-0306.2016.04.056

Optimization study on purification of polysaccharides in polygonatum odoratum

doi: 10.13386/j.issn1002-0306.2016.04.056
  • Received Date: 2015-05-25
  • Single factor experiment and orthogonal experiment were undertaken to study the best deproteinization methods of polygonatum polysaccharides. On this basis,by contrasting the decolorizing effects of polygonatum polysaccharides of the different types of resin,choose the most suitable decolorization resin type and the optimal dynamic parameters. Enzymatic and sevag method combination was the best deproteinization. The results showed that the enzyme dosage of 0.1 g,enzymolysis temperature of 40 ℃,enzymolysis time of 1 h,solution p H of 5,the loss rate of polysaccharide was 13.14% and the protein removal rate was 72.43%. Under the conditions of using decolorization 1 resin,sample concentration 4 mg/m L,flow rate 1 m L/min,sample loading amount 2 BV,the decolorization rate of polygonatum polysaccharides was 80.65%,and the retention rate of the polysaccharides was 87.82%.
  • loading
  • [1]
    刘塔斯.玉竹[M].北京:中国中医药出版社,2001:82.
    [2]
    谢建军,王长松,胡蔓菁.玉竹多糖预处理对糖尿病大鼠胰岛β细胞损伤的影响[J].中国医院药学杂志,2010,30(14):200-1203.
    [3]
    单颖,姜东,潘兴瑜,等.玉竹多糖对衰老模型鼠细胞及体液免疫功能的影响[J].中国临床康复,2006,10(19):146-148.
    [4]
    江苏新医学院.中药大辞典[M].上海:上海人民出版社,1977:1156.
    [5]
    竺平晖,陈爱萍.GC-MS法对湖南产玉竹挥发油成分的分析研究[J].中草药2010,41(8):1264-1265.
    [6]
    陈振兴,陈地灵,林励,等.利用D-900树脂对巴戟天多糖脱色工艺进行优化[J].食品科学,2012,33(8):71-75.
    [7]
    李艳辉,鲁巍巍,官艳丽,等.玉竹多糖脱蛋白方法的比较研究[J].时珍国医国药,2011,9:2127-2128.
    [8]
    李新华,杨强,王琳.微波辅助提取银杏白果多糖的工艺研究[J].食品科技,2012,37(4):160-163
    [9]
    杨强,李新华,林子木.银杏果多糖树脂脱色工艺[J].食品与发酵工业,2012,38(12):107-110.
    [10]
    袁红波,张劲松,贾薇,等.利用大孔树脂对低分子量灵芝多糖脱色的研究[J].食品工业科技,2009,30(3):204-206.
    [11]
    黄艳伟,张雪红,姚先超,等.剑麻果胶多糖脱蛋白方法研究[J].食品科技,2011,36(2):144-147.
    [12]
    WANG Xia,YUAN Yong,WANG Kainai,et al.Deproteinization of gellan gum produced by Sphingomonas paucimobilis ATCC 31461[J].Journal of Biotechnology,2007,128(2):403-407.
    [13]
    王传名,董祺,管从胜.日照绿茶粗多糖脱蛋白研究[J].食品工业科技,2010(8):274-276.
    [14]
    刘海霞,牛鹏飞,王峰,等.大孔吸附树脂对大枣多糖提取液的脱色条件研究[J].食品与发酵工业,2007,33(10):180-184.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (71) PDF downloads(391) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return