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ZHANG Hong, MA Wei- chao, CHEN Ke- quan. Study on the high- throughput detection of cadaverine[J]. Science and Technology of Food Industry, 2016, (05): 283-286. doi: 10.13386/j.issn1002-0306.2016.05.048
Citation: ZHANG Hong, MA Wei- chao, CHEN Ke- quan. Study on the high- throughput detection of cadaverine[J]. Science and Technology of Food Industry, 2016, (05): 283-286. doi: 10.13386/j.issn1002-0306.2016.05.048

Study on the high- throughput detection of cadaverine

doi: 10.13386/j.issn1002-0306.2016.05.048
  • Received Date: 2015-08-27
  • In this paper,to develop a high- throughput bioamine detection method based on spectrophotometry,the cadaverine concentration in a whole- cell bioconversion solution was determined,and the extracting agent,reaction temperature and time were optimized. The results showed that the use of p- xylene as the extraction agent was more conducive to eliminate the influence of the L- lysine on bioamine detection,and the optimal conditions for the derivatization of cadaverine with 2,4,6- trinitrobenzene sulfonic acid( TNBS) were 42 ℃ and 6 min. By using this method,a standard curve with a correlation coefficient( R2) of 0.9988 was obtained,and the linear range was 4.176~20.88 μg.The results of validation studies showed that,this method was precise( RSD = 1.722%) and reproducible( RSD = 6.414%),and the recovery was 100.5%. This method was simple,time- saving and suitable for the conventional rapid quantitative detecting of biogenic amines.
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  • [1]
    王颖,邱璠,韩北忠,等.食品中的生物胺及其检测方法[J].中国酿造,2011(10):1-4.
    [2]
    吴勤学.5-羟色胺在皮肤病中的意义[J].国外医学参考资料.皮肤病学分册,1975(1):24-26.
    [3]
    刘辰麒,丁卓平,王锡昌.生物胺的检测方法评价[J].现代科学仪器,2006(4):89-91.
    [4]
    Kind S,Neubauer S,Becker J,et al.From zero to hero-Pr oduction of bio-based nylon from renewable resources using engineered Corynebacterium glutamicum[J].Metabolic Engineering,2014(25):113-123.
    [5]
    Ma WC,Cao WJ,Zhang H,et al.Enhanced cadaverine production from L-lysine using recombinant Escherichia coli cooverexpressing Cad A and Cad B[J].Biotechnology Letters,2015,37(4):799-806.
    [6]
    Li NQ,Chou HW,Yu LJ,et al.Cadaverine production by heterologous expression of Klebsiella oxytoca lysine decarboxylase[J].Biotechnology and Bioprocess Engineering,2014,19(6):965-972.
    [7]
    Proestos C,Loukatos P,Komaitis M.Determination of biogenic amines in wines by HPLC with precolumn dansylation and fluorimetric detection[J].Food Chemistry,2008,106(3):1218-1224.
    [8]
    Lorenzo JM,Cachaldora A,Fonseca S,et al.Production of biogenic amines“in vitro”in relation to the growth phase by Enterobacteriaceae species isolated from traditional sausages[J].Meat Science,2010,86(3):684-691.
    [9]
    Fernandes JO,Ferreir MA.Combined ion-pair extraction and gas chromatography-mass spectrometry for the simultaneous determination of diamines,polyamines and aromatic amines in Port wine and grape juice[J].Journal of Chromatography A,2000,886(1-2):183-195.
    [10]
    Shalaby AR.Simple,rapid and valid thin layer chromatographic method for determining biogenic amines in foods[J].Food Chemistry,1999,65(1):117-121.
    [11]
    Phan APH,Ngo TT,Lenhoff HM.Spectrophotometric Assay for Lysine Decarboxylase[J].Analytical Biochemistry,1982,120:193-197.
    [12]
    刘洪涛,吴洪,曹永兵,等.衍生化-毛细管气相色谱法定量分析白念珠菌中甾醇含量[J].药物分析杂志,2002(10):264-267.
    [13]
    高虹,周杨,孙远明,等.HPLC法检测细胞培养上清液中前列腺素E2的含量[J].食品工业科技,2012(1):326-328.
    [14]
    伍云卿,涂杰峰,范超,等.加标回收实验方案探讨[J].福建分析测试,2010,19(3):67-71.
    [15]
    张虹.加标回收率的测定和结果判断[J].石油与天然气化工,2000(1):50-52.
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