ZHANG Hong, MA Wei- chao, CHEN Ke- quan. Study on the high- throughput detection of cadaverine[J]. Science and Technology of Food Industry, 2016, (05): 283-286. doi: 10.13386/j.issn1002-0306.2016.05.048
Citation:
ZHANG Hong, MA Wei- chao, CHEN Ke- quan. Study on the high- throughput detection of cadaverine[J]. Science and Technology of Food Industry, 2016, (05): 283-286. doi: 10.13386/j.issn1002-0306.2016.05.048
ZHANG Hong, MA Wei- chao, CHEN Ke- quan. Study on the high- throughput detection of cadaverine[J]. Science and Technology of Food Industry, 2016, (05): 283-286. doi: 10.13386/j.issn1002-0306.2016.05.048
Citation:
ZHANG Hong, MA Wei- chao, CHEN Ke- quan. Study on the high- throughput detection of cadaverine[J]. Science and Technology of Food Industry, 2016, (05): 283-286. doi: 10.13386/j.issn1002-0306.2016.05.048
In this paper,to develop a high- throughput bioamine detection method based on spectrophotometry,the cadaverine concentration in a whole- cell bioconversion solution was determined,and the extracting agent,reaction temperature and time were optimized. The results showed that the use of p- xylene as the extraction agent was more conducive to eliminate the influence of the L- lysine on bioamine detection,and the optimal conditions for the derivatization of cadaverine with 2,4,6- trinitrobenzene sulfonic acid( TNBS) were 42 ℃ and 6 min. By using this method,a standard curve with a correlation coefficient( R2) of 0.9988 was obtained,and the linear range was 4.176~20.88 μg.The results of validation studies showed that,this method was precise( RSD = 1.722%) and reproducible( RSD = 6.414%),and the recovery was 100.5%. This method was simple,time- saving and suitable for the conventional rapid quantitative detecting of biogenic amines.
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