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WANG Bo, ZHANG Meng-shan, ZHANG Zhong-yu, LI Shan-shan. Study on extraction,purification and antitumor activity of polysaccharide from bee pollen of Dendranthema indicum[J]. Science and Technology of Food Industry, 2016, (05): 358-360. doi: 10.13386/j.issn1002-0306.2016.05.064
Citation: WANG Bo, ZHANG Meng-shan, ZHANG Zhong-yu, LI Shan-shan. Study on extraction,purification and antitumor activity of polysaccharide from bee pollen of Dendranthema indicum[J]. Science and Technology of Food Industry, 2016, (05): 358-360. doi: 10.13386/j.issn1002-0306.2016.05.064

Study on extraction,purification and antitumor activity of polysaccharide from bee pollen of Dendranthema indicum

doi: 10.13386/j.issn1002-0306.2016.05.064
  • Received Date: 2015-08-27
  • The purification procedures,physiochemical features and antitumor activity of polysaccharide fractions from bee pollen of Dendranthema indicum were studied in this paper.Polysaccharide fractions( WDPP) was dealed with water-extraction,ethnol-precipetation and ion- exchange chromatography,then one netral( WDPP- N) and two acidic fractions( WDPP-1,WDPP-2) were obtained.The monosaccharide composition was tested through HPLC,the antitumor activity of all fractions were investigated by MTT method.WDPP-N was composed of galactose,arabinose,and glucose with a ratio of 30.3 ∶ 31.6 ∶ 32.7; WDPP- 1 and WDPP- 2 were mainly composed by galacturonic acid,galactose and arabinose,rhamose and glucose as small proportion.Total polysaccharide had obvious inhibition effect on HT-29 and HCT116 tumor cells in high concentration compared with neutral and acidic fractions.
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