马尾藻岩藻聚糖硫酸酯降解工艺的研究

Study on the degradation conditions of fucoidan from Sargassum henslowianum

摘要: 利用超声波降解法,以还原糖释放量为指标,对超声波功率、超声波时间及温度与多糖(马尾藻岩藻聚糖硫酸酯SF1)降解率的相关性进行分析,用响应面设计优化降解条件,建立了多糖降解率与超声波功率、时间和温度之间的二次回归模型。选择超声功率350W,温度50℃,时间59min,还原糖释放量为32.86μg/mL;降解后总糖含量为70.55%,岩藻糖含量为37.62%,硫酸根含量为36.12%,糖醛酸含量为6.15%。

Abstract: The degradation of SF1 from Sargassum hemiphyllum was depended on ultrasound.Reducing sugar release was the indicator to reflect the relativity among ultrasound power, temperature and time during the degradation.Response surface methodology was used to optimize the degradation conditions.The result showed that the behavior of the system was explained by a quadratic equation between reducing sugar release and factors (ultrasonic power, temperature and time) .The result showed degradation conditions were ultrasonic power 350W, temperature 50℃, time 59min, and the reducing sugar release was 32.86μg/mL.Polysaccharide content, fucose content, sulfate group content and uronic acid of SF1' were 70.55%, 37.62%, 36.12% and 6.15%.