Abstract: Objective:To study the α-glucosidase inhibitory activity of extracts from six varieties of Cucurbita moschata Duch. (Jingou, Tianmian, Riben, Chaotianmiben, Miben and Liaoningxinminjingou) . Methods:By established α-glucosidase inhibitory model in vitro, the activity of extracts from C. moschata was screened. The relationship of inhibitory ratio and the extract concentration were also studied. Results:Different extracts from six varieties of C. moschata all had inhibitory activity of α-glucosidase, among which petroleum ether extract of C. moschata (Jingou) had the highest inhibitor activity of α-glucosidase (IC50=143.91μg/mL) , which was lower than that of Acarbose (IC50=1103.01μg/mL) . Among different extracts inhibitor activity of petroleum ether extract was higher than that of ethyl acetate extract and methanol extract. Conclusion:The extracts from six varieties of C. moschata all had inhibitory activity of α-glucosidase, but different varieties of the inhibition rate had certain difference.