鲁米诺-铁氰化钾流动注射化学发光体系测定饮用水中ɑ-茄碱

Determination of a-solanine in drinking water by flow-injection chemiluminescence system of luminol and potassium ferricyanide

摘要: 建立一种快速测定ɑ-茄碱的新方法,利用鲁米诺-铁氰化钾化学发光体系,结合流动注射技术,对ɑ-茄碱进行测定。实验结果表明,ɑ-茄碱对鲁米诺-铁氰化钾体系发光反应有明显的抑制作用,在最佳实验条件下,该方法的线性范围为8.0×10-4～1.0×10-1mg/mL,检出限为6.09×10-5mg/mL,RSD为2.1%（n=11）。该方法具有操作简单、自动化程度高、成本低廉、容易实现在线检测以及即时预警等优点,可用于饮用水中ɑ-茄碱的快速检测。

Abstract: In order to establish a new method for rapid determination of a-solanine, a flow-injection chemiluminescence (FI-CL) system of luminol and potassium ferricyanide was developed.Results indicated that the CL reaction betweeen luminol and potassium ferricyanide was significantly reduced by a-solanine.An excellent linear range for a-solanine determination was 8.0×10-4～1.0×10-1mg/mL under the optimal condition.The limit detection of a-solanine was 6.09×10-5mg/mL and relative standard deviation for the determination was 2.1% (n=11) .This method showed the advantages of simple operation, high degree of automation, easy to run on-line and real-time warning detection, and could be used as a rapid method for determination of a-solanine in drinking water.