Abstract: Uniform Design was applied to optimize the extraction of Cornus officinalis seed oil with ultrasonic extraction. The fatty acid profile of the oil was analyzed by GC. The optimal extraction conditions were 22min, 136W, 52℃, 1∶29. Under these conditions, the yield of oil was 7.366%. A total of 6 fatty acids were identified in the oil, with a relative content of 99.013% in total fatty acids. Linoleic acid (C18 ∶2, 71.190% ) was the most predominant fatty acid in theoil , followed by oleic acid ( C18 ∶ 0 , 18 . 594 % ) , cis-10-pentadecenoic acid (C15∶1, 5.377%) , stearic acid (2.176%) , α-linolenic acid (1.226%) and cis-11-eicosenoate acid (C20∶1 0.450%) .The experimental results showed that the oil from Cornus officinalis were rich in unsaturated fatty acid and had a high nutritional value.