Abstract: The brown granulated sugar syrup was clarified via chitosan and γ-polyglutamic acid cooperative flocculation.The first process was decolorized by chitosan.In the second step, the γ-polyglutamic acid was used to coagulation and decoloration.Under these experiment conditions, the perfect decoloration effect was obtained.By single-factor and multi-factor orthogonal experiment methods, the content of chitosan, the effects of pH, the content of γ-polyglutamic acid, and the reaction temperature on decoloration efficiency were investigated.The results showed that the optimal process conditions for chitosan and γ-polyglutamic acid clarification were chitosan dosage 0.5g/L, γ-polyglutamic acid dosage 0.06g/L, reaction pH5.0, reaction temperature 20℃.Under these optimal conditions, a clarification rate of 66.5% was achieved.