蛹虫草子实体多糖抗突变作用
Study on the anti-mutagenic effect of Cordyceps militaris polysaccharide
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摘要: 研究蛹虫草多糖(CMPS)对环磷酰胺(CTX)诱导小鼠骨髓嗜多染红细胞(PCE)微核形成率及染色体畸变率的影响,探讨其抗突变作用。采用微核实验和染色体畸变实验技术,镜下观察并计数。结果显示:CMPS三个剂量组小鼠微核抑制率分别为29.75%、36.20%、44.48%,抑制率随剂量增多呈增加趋势,各剂量组与环磷酰胺组比较,均有显著性差异(p<0.05);CMPS三个剂量组染色体畸变率分别为36.7%±5.46%、20.8%±3.52%、18.1%±3.28%,畸变率随CMPS剂量增多呈下降趋势。蛹虫草多糖可降低环磷酰胺诱导小鼠骨髓嗜多染红细胞微核率及染色体畸变率,具有抗突变活性。Abstract: To evaluate the effect of Cordyceps militaris polysaccharides (CMPS) on micronucleus formation rate of polychromatic erythrocyte (PCE) of bone marrow cells in mice and chromosome aberration rates induced by cyclophosphamide (CTX) . Micronucleus test and chromosomal aberration test were carried out. The micronucleus inhibition rates were 29. 75%, 36. 20% and 44. 48% respectively in CMPS1, CMPS2 and CMPS3 group. Inhibition rates were increased with the dose increasing. Compared with the CTX group, each dose group were significantly different (p<0. 05) . The aberration rates which were decreased with the dose increasing were 36. 7%±5. 46%, 20. 8%±3. 52% and 18. 1%±3. 28% respectively in CMPS1, CMPS2 and CMPS3 group. CMPS could reduce the micronucleus inhibition rates of PCE of bone marrow cells in mice and the aberration rates induced by CTX, CMPS had anti-mutagenic activity.
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